CAJ | 학술논문

目的比较富血小板纤维蛋白(platelet-rich fibrin,PRF)与富血小板血浆(platelet-rich plasma,PRP)的成骨效果,探讨应用PRF修复骨缺损的方法. 方法选择16只新西兰大白兔,在每只大白兔的颅顶骨上各制备4个直径为7 mm的骨缺损区,分为A、B、C、D区,然后根据填人材料的不同进行分组:A区填入PRF(PRF组)、B区填入PRF可吸收骨材料Bio-Oss骨粉(B0)(PRF+ BO组)、C区填入PRP+ BO(PRP+ BO组),D区填入PRP(PRP组),每组4只,术后2,4,8,12周分别行大体观察、X线片及组织学观察缺损区变化;并进行缺损区骨密度和新生骨小梁面积分析(均以灰度值表示). 结果 2周各缺损区已出现骨修复;4,8,12周各组缺损区骨修复效果均依次向好,其中B区最好,其次C区,A区好于D区.术后2周,A、B、C、D区骨小梁面积分别为10.95 ±0.58,15.45 ±0.79,10.22 ±0.43,6.58±0.64,任意两组比较差异均有统计学意义(F=22.869,P＜0.01);术后4,8周,各组骨小梁面积均增加;12周时,A、B、C、D区骨小梁面积依次为35.09±0.58,59.44 ±0.60,50.75±1.56,30.94±1.19,任意两组比较差异均有统计学意义(F=1 002.904,P＜0.01),结果显示,骨修复效果B区最好,其次C区,A区好于D区. 结论 PRF的成骨效果优于PRP,并且PRF复合BO修复骨缺损效果更好.
목적 비교부혈소판섬유단백(platelet-rich fibrin,PRF)여부혈소판혈장(platelet-rich plasma,PRP)적성골효과,탐토응용PRF수복골결손적방법. 방법 선택16지신서란대백토,재매지대백토적로정골상각제비4개직경위7 mm적골결손구,분위A、B、C、D구,연후근거전인재료적불동진행분조:A구전입PRF(PRF조)、B구전입PRF가흡수골재료Bio-Oss골분(B0)(PRF+ BO조)、C구전입PRP+ BO(PRP+ BO조),D구전입PRP(PRP조),매조4지,술후2,4,8,12주분별행대체관찰、X선편급조직학관찰결손구변화;병진행결손구골밀도화신생골소량면적분석(균이회도치표시). 결과 2주각결손구이출현골수복;4,8,12주각조결손구골수복효과균의차향호,기중B구최호,기차C구,A구호우D구.술후2주,A、B、C、D구골소량면적분별위10.95 ±0.58,15.45 ±0.79,10.22 ±0.43,6.58±0.64,임의량조비교차이균유통계학의의(F=22.869,P＜0.01);술후4,8주,각조골소량면적균증가;12주시,A、B、C、D구골소량면적의차위35.09±0.58,59.44 ±0.60,50.75±1.56,30.94±1.19,임의량조비교차이균유통계학의의(F=1 002.904,P＜0.01),결과현시,골수복효과B구최호,기차C구,A구호우D구. 결론 PRF적성골효과우우PRP,병차PRF복합BO수복골결손효과경호.
Objective To compare the osteogenesis effect of platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) and investigate the methods of repairing bone defect with PRF.Methods Four defects measuring 7 mm in diameter were made in the parietal bone of 16 New Zealand white rabbits.The defects named A,B,C,and D and were filled with PRF,PRF-mixed Bio-Oss (BO),PRP-mixed BO,and PRP separately.Every four rabbits were sacrificed at postoperative 2,4,8,and 12 weeks and defects were examined grossly,radiographically,and histologically.Besides,bone mineral density and bone trabecular area were determined and expressed as gray-scale values.Results Newly regenerated bone appeared at all defect areas at postoperative 2 weeks.Thereafter,more bone formations were observed over time and area B demonstrated the best bone healing followed by area C,A,and D in succession.Bone trabecular area in areas A,B,C,and D was 10.95 ± 0.58,15.45 ± 0.79,10.22 ± 0.43,and 6.58 ± 0.64 at postoperative 2 weeks with significant differences in pair comparison (F =22.869,P ＜0.01),followed by some increase at postoperative 4 and 8 weeks.Whereas,bone trabecular area in areas A,B,C,and D increased largely at postoperative 12 weeks (35.09 ± 0.58,59.44 ± 0.60,50.75 ± 1.56,and 30.94 ± 1.19) and showed significant difference when compared in a pair (F =1 002.904,P ＜ O.01).Conclusion PRF is superior to PRP in promoting bone formation,but a much better effect of PRF/BO composite is observed in bone repair.