CAJ | 학술논문

目的评估富集骨髓基质干细胞(BMSCs)复合β-磷酸三钙(β-TCP)在促进骨缺损修复中的作用. 方法抽取12只崇明山羊骨髓血,用梯度离心法分别将其中的BMSCs富集、分离后与β-TCP复合.12只山羊双侧股骨内髁钻孔,制备骨缺损模型.所有山羊左侧股骨内髁缺损通过富集BMSCs/β-TCP进行局部填充治疗(实验组),其中10只羊右侧股骨内髁缺损以单纯β-TCP颗粒填充治疗(对照组),2只羊右侧股骨内髁缺损旷置(空白组),评价富集效率.术后16周处死取材,将所得标本进行影像学观察和形态学计量,实验组和对照组之间进行比较. 结果 BMSCs富集后有核细胞数和碱件磷酸酶染色呈阳性的细胞集落形成单位(CFUs/ALP+)数均有显著提高,差异有统计学意义(P<0.05).X线侧位片和μ-CT形态计量分析显示实验组新生骨长入率(70.27%±8.47%)显著高于对照组(21.40%±5.77%),差异有统计学意义(t=11.97,P<0.05).X线侧位片、CT平扫与三维重建以及μ-CT图像均可见实验组疗效优于对照组. 结论在促进骨缺损修复中,富集BMSCs/β-TCP的疗效优于单纯β-TCP颗粒,有良好的应用前景.
목적 평고부집골수기질간세포(BMSCs)복합β-린산삼개(β-TCP)재촉진골결손수복중적작용. 방법 추취12지숭명산양골수혈,용제도리심법분별장기중적BMSCs부집、분리후여β-TCP복합.12지산양쌍측고골내과찬공,제비골결손모형.소유산양좌측고골내과결손통과부집BMSCs/β-TCP진행국부전충치료(실험조),기중10지양우측고골내과결손이단순β-TCP과립전충치료(대조조),2지양우측고골내과결손광치(공백조),평개부집효솔.술후16주처사취재,장소득표본진행영상학관찰화형태학계량,실험조화대조조지간진행비교. 결과 BMSCs부집후유핵세포수화감건린산매염색정양성적세포집락형성단위(CFUs/ALP+)수균유현저제고,차이유통계학의의(P<0.05).X선측위편화μ-CT형태계량분석현시실험조신생골장입솔(70.27%±8.47%)현저고우대조조(21.40%±5.77%),차이유통계학의의(t=11.97,P<0.05).X선측위편、CT평소여삼유중건이급μ-CT도상균가견실험조료효우우대조조. 결론 재촉진골결손수복중,부집BMSCs/β-TCP적료효우우단순β-TCP과립,유량호적응용전경.
Objective To access the ability of rich bone marrow stromal cells combined with porous beta-tricalcium phosphate (enriched BMSCs/β-TCP) to repair large bone defects of goat femoral condyle. Methods The bone marrow was harvested from 12 Chongming goats, from which the BMSCs were isolated and condensed with density gradient centrifugation method. The enriched BMSCs were then mixed with β-TCP. Animal models of bone defects were produced by drilling holes in the femoral medial condyle in each of the 12 goats. The left side was treated with enriched BMSCs/β-TCP in 12 goats (experimental group), while the right side with β-TCP alone (control group) in 10 goats and untreated (blank control group) in the other 2 goats. The enrichment efficiency was evaluated. After euthanasia at 16 weeks after surgery, radio-logical observations and morphometry were used to compare the situations between the experimental and control groups. Results After enrichment and isolation, the nucleated cells (NCs) and colony-forming units/ alkaline phosphatase + (CFUs/ALP + ) were increased significantly. X-ray lateral projection, CT and three-dimensional reconstruction, μ-CT images revealed that the effect of enriched BMSCs/β-TCP was better than that of β-TCP alone. X-ray lateral projection and μ-CT morphometry showed that new bone formation was higher in the experimental group. Conclusion Enriched BMSCs/β-TCP is effective in repairing bone defects and may have a promising clinical application.