CAJ | 학술논문

目的检测玉米醇溶蛋白(zein)仿生三维多孔支架材料的生物相容性、骨诱导性和生物可降解性. 方法将人骨髓基质干细胞(BMSCs)载人海藻酸钠或zein制成复合物.通过扫描电镜观察、噻唑蓝分析法以及碱性磷酸酶染色与定量检测,分别比较zein和海藻酸钠对BMSCs体外黏附、增殖和分化的影响.取24只8周龄裸鼠,随机分为两组,制成肌袋植入异位成骨模型.对照组单纯植入zein,实验组植入zein与兔BMSCs复合物.术后2、4、8和12周取材进行影像学和组织学观察. 结果与海藻酸钠相比,zein在任何时间段均能够更好地促进兔BMSCs的体外增殖和分化,差异有统计学意义(P<0.05).同时,与单纯植入zein相比,BMSCs能够增强zein体内诱导成骨的作用. 结论 zein具有良好的生物相容性、骨诱导性和生物可降解性.
목적 검측옥미순용단백(zein)방생삼유다공지가재료적생물상용성、골유도성화생물가강해성. 방법 장인골수기질간세포(BMSCs)재인해조산납혹zein제성복합물.통과소묘전경관찰、새서람분석법이급감성린산매염색여정량검측,분별비교zein화해조산납대BMSCs체외점부、증식화분화적영향.취24지8주령라서,수궤분위량조,제성기대식입이위성골모형.대조조단순식입zein,실험조식입zein여토BMSCs복합물.술후2、4、8화12주취재진행영상학화조직학관찰. 결과 여해조산납상비,zein재임하시간단균능구경호지촉진토BMSCs적체외증식화분화,차이유통계학의의(P<0.05).동시,여단순식입zein상비,BMSCs능구증강zein체내유도성골적작용. 결론 zein구유량호적생물상용성、골유도성화생물가강해성.
Objective To evaluate the biocompatibility, osteoinductivity and biodegradability of porous zein scaffolds. Methods Bone marrow stromal cells (BMSCs) were isolated from hBMSCs and seeded on alginate and zein respectively. SEM, MTT, and ALP staining and quantitation were utilized to compare the biocompatibilities of alginate and zein. The bilateral thigh muscle pouches were made models of ectopic bone formation in 24 nude mice and divided into equal 2 groups randomly. The muscle pouches were implanted with simple zein scaffolds in group A and with zein scaffolds plus rabbit BMSCs in group B. The effects of ectopic bone formation and scaffold degradation were evaluated by radiographic and histological ex-aminations at 2, 4, 8, 12 and 24 weeks after operation. Results SEM illustrated that BMSCs spread well on zein. Besides, BMSCs seeded on zein showed better proliferation and differentiation than on alginate. In models of ectopic bone formation, both X-ray and histological results demonstrated that zein alone could enhance new bone formation and the osteoinducitivity could be dramatically enhanced by seeding BMSCs. Conclusion Since zein has good biocompatibility, osteoinductivity and biodegradation, it can be a prospective choice for autologous bone grafts.