CAJ | 학술논문

目的通过探讨左旋咪唑(LMS)对骨折创伤后机体红细胞生物学行为的影响,为预防和治疗创伤后深静脉血栓的形成提供新的思路. 方法选取30只成年健康雄性家兔随机分为两组(n=15):创伤对照组(A组)与创伤用药组(B组).麻醉后,两组家兔均于小转子下方3 cm截骨并用钢板螺钉固定,其中A组不予处理,B组于术前30 min按照24 mg/kg肌肉注射LMS.所有家兔均于术前30 min,术后30 min、1 d、4 d、7 d抽取血样,分别测定红细胞丙二醛含量;检测不同时间点红细胞Ⅰ型补体受体花环率(RC3bRR)、红细胞免疫复合物花环率(RICR)、自然肿瘤红细胞花环率(NTERR),以及血流变性、红细胞刚性指数、聚集指数、变形指数等. 结果红细胞免疫在术后30 min、1 d、4 dRC3bRR、NTERR B组比A组同时相高,而RICR B组比A组同时相低,差异均有统计学意义(P<0.05);以上三项指标A组术后较术前差异有统计学意义(P<0.05),B组术后较术前差异无统计学意义(P>0.05).红细胞脂质过氧化在术后30 min、1 d、4 d红细胞中丙二醛均比术前高,同时B组比A组同时相低,差异均有统计学意义(P<0.05);A组术后较术前差异有统计学意义(P<0.05),B组术后较术前差异无统计学意义(P>0.05). 结论创伤能引起机体红细胞免疫功能降低,脂质过氧化增强,流变性变差;LMS可以明显改善创伤后红细胞生物学行为,即可能对于降低骨折创伤后深静脉血栓的形成提供新的思路.
목적 통과탐토좌선미서(LMS)대골절창상후궤체홍세포생물학행위적영향,위예방화치료창상후심정맥혈전적형성제공신적사로. 방법 선취30지성년건강웅성가토수궤분위량조(n=15):창상대조조(A조)여창상용약조(B조).마취후,량조가토균우소전자하방3 cm절골병용강판라정고정,기중A조불여처리,B조우술전30 min안조24 mg/kg기육주사LMS.소유가토균우술전30 min,술후30 min、1 d、4 d、7 d추취혈양,분별측정홍세포병이철함량;검측불동시간점홍세포Ⅰ형보체수체화배솔(RC3bRR)、홍세포면역복합물화배솔(RICR)、자연종류홍세포화배솔(NTERR),이급혈류변성、홍세포강성지수、취집지수、변형지수등. 결과 홍세포면역재술후30 min、1 d、4 dRC3bRR、NTERR B조비A조동시상고,이RICR B조비A조동시상저,차이균유통계학의의(P<0.05);이상삼항지표A조술후교술전차이유통계학의의(P<0.05),B조술후교술전차이무통계학의의(P>0.05).홍세포지질과양화재술후30 min、1 d、4 d홍세포중병이철균비술전고,동시B조비A조동시상저,차이균유통계학의의(P<0.05);A조술후교술전차이유통계학의의(P<0.05),B조술후교술전차이무통계학의의(P>0.05). 결론 창상능인기궤체홍세포면역공능강저,지질과양화증강,류변성변차;LMS가이명현개선창상후홍세포생물학행위,즉가능대우강저골절창상후심정맥혈전적형성제공신적사로.
Objective To investigate the influence of levamisole (LMS) on the biological behavior of red blood cells (RBC) after fracture, in order to suggest a new strategy for prevention and therapy of traumatic deep venous thrombosis (DVT) . Methods Thirty male rabbits were divided randomly into 2 equal groups, control group A and experimental group B. After anesthesia, fractures were created 3 cm below the entotrochanter and then fixed with plates and screws in both groups. Blood samples were harvested from the 2 groups to analyze blood rheological characteristics respectively at 30 min before operation, 30 min, 1, 4 and 7 days after operation. Malondialdehyde (MDA), rigid index, accumulation index and distortion index, C3b receptor rate (RC3bRR), circulating immune complexes rate (RICR) and natural tumor erythrocyte rosette rate (NTERR) of RBCs were measured. Results RBC immunity: The rates of RC3BRR and NTERR in group B were higher than in the control group (group A) (P < 0.05) at 30 min, 1 and 4 days after operation. However, the rate of RICR was higher in group A than in group B (P < 0.05). There were significant dif-ferences between pre- and post-operation in group A, but not in group B. RBC lipid peroxidation: The post-operative MDA values of RBCs at 30 min, 1 and 4 days in both groups were higher than the preoperative one. The differences were statistically significant (P < 0.05) in group A, but not in group B. The MDA was lower in group B than in group A (P < 0.05). Conclusions Trauma can weaken the RBC immune function, increase the lipid perexidation, and worsen the blood rheological property. LMS can obviously improve the biological behavior of RBC, suggesting it may be useful in prevention and therapy of DVT after fracture.