CAJ | 학술논문

目的通过立体定向和尾静脉移植骨髓基质干细胞(BMSCs)于脊髓损伤(SCI)大鼠体内,观察移植后诱发电位的变化及移植细胞对大鼠SCI神经功能恢复的影响. 方法体外分离、纯化、扩增大鼠BMSCs,移植前用免疫组化单染BrdU标记.动脉瘤夹钳夹法制作大鼠SCI模型.将45只健康成年雌性SD大鼠随机分为对照组、静脉移植组和立体定向移植组(n=15).对照组仅造模,静脉移植组和立体定向移植组在致伤7d后分别经尾静脉和立体定向途径将标记BrdU的BMSCs悬液植入SCI大鼠体内.通过神经电生理检测比较不同途径移植BMSCs对SCI大鼠神经功能恢复的影响. 结果三组大鼠造模前和造模后7d诱发电位潜伏期及波幅比较,差异均无统计学意义(P＞0.05)；造模后30 d所有大鼠潜伏期逐渐出现不同程度缩短,而波幅逐渐出现不同程度升高.立体定向移植组与静脉移植组大鼠运动诱发电位、感觉诱发电位恢复均优于对照组,且立体定向移植组大鼠运动诱发电位恢复优于静脉移植组,差异有统计学意义(P＜0.05).结论 BMSCs经不同途径移植均可在大鼠脊髓内存活、迁移,明显改善SCI大鼠的神经功能缺损,且移植等量BMSCs时立体定向移植疗效优于尾静脉移植.
목적 통과입체정향화미정맥이식골수기질간세포(BMSCs)우척수손상(SCI)대서체내,관찰이식후유발전위적변화급이식세포대대서SCI신경공능회복적영향. 방법 체외분리、순화、확증대서BMSCs,이식전용면역조화단염BrdU표기.동맥류협겸협법제작대서SCI모형.장45지건강성년자성SD대서수궤분위대조조、정맥이식조화입체정향이식조(n=15).대조조부조모,정맥이식조화입체정향이식조재치상7d후분별경미정맥화입체정향도경장표기BrdU적BMSCs현액식입SCI대서체내.통과신경전생리검측비교불동도경이식BMSCs대SCI대서신경공능회복적영향. 결과 삼조대서조모전화조모후7d유발전위잠복기급파폭비교,차이균무통계학의의(P＞0.05)；조모후30 d소유대서잠복기축점출현불동정도축단,이파폭축점출현불동정도승고.입체정향이식조여정맥이식조대서운동유발전위、감각유발전위회복균우우대조조,차입체정향이식조대서운동유발전위회복우우정맥이식조,차이유통계학의의(P＜0.05).결론 BMSCs경불동도경이식균가재대서척수내존활、천이,명현개선SCI대서적신경공능결손,차이식등량BMSCs시입체정향이식료효우우미정맥이식.
Objective To compare the effects of stereotaxis and vena caudalis transplantations of bone marrow mesenchymal stem cells(BMSCs) in the treatment of spinal cord injury (SCI) in rats.Methods SD rat BMSCs were isolated,purified and proliferated in vitro.Forty-five adult female Wistar rats were used to establish SCI models with aneurysm clips before they were randomly divided into 3 even groups:a control group and stereotaxis and vena caudalis transplantation groups.The SCI was not treated in the control group.Seven days after the SCI,the suspension with BrdU-labeled BMSCs was injected into the SCI site in the stereotaxis transplantation group and into the body through the caudal vein in the vena caudalis transplantation group.Neural electrophysiologic tests were used to compare the effects of the 2 different ways of BMSCs transplantation on the neural recovery of the injured spinal cord in rats.Results There were no significant differences among the 3 groups before establishment of the SCI model and 7 days after the establishment of SCI model regarding the latency and amplitude of evoked potentials (P ＞ 0.05).Thirty days after the establishment of SCI model all the rats showed significantly reduced latency and significantly increased amplitude (P ＜ 0.05).The BMSCs transplantation groups showed significantly shorter motor evoked potential latency and shorter somatosensory evoked potential P1 wave latency than the control group (P ＜ 0.05).The recovery of motor evoked potentials was significantly slower in the vena caudalis transplantation group than in the stereotaxis transplantation group (P ＜ 0.05).Conclusions Transplanted BMSCs can survive,migrate in the host spinal cord,leading to significant recovery of neurological function in SCI rats.With the same amount of BMSCs,stereotaxis transplantation may be more effective than vena caudalis transplantation in the SCI treatment in rats.