中华创伤骨科杂志
中華創傷骨科雜誌
중화창상골과잡지
CHINESE JOURNAL OF ORTHOPAEDIC TRAUMA
2013年
4期
336-340
,共5页
邹继伟%王德欣%李亮%吕敏%李丹%毕龙%裴国献
鄒繼偉%王德訢%李亮%呂敏%李丹%畢龍%裴國獻
추계위%왕덕흔%리량%려민%리단%필룡%배국헌
骨基质%骨髓细胞%组织支架%去蛋白
骨基質%骨髓細胞%組織支架%去蛋白
골기질%골수세포%조직지가%거단백
Bone matrix%Bone marrow cells%Tissue scaffolds%Deproteinization
目的 比较牛去蛋白脱钙皮质骨基质(bDpDBM)微颗粒和牛脱钙皮质骨基质(bDBM)微颗粒用于体外干细胞培养的优缺点,探讨其作为微载体制备候选材料的可行性. 方法 冷冻粉碎牛四肢皮质骨,筛选出直径为200 ～ 500μm的微颗粒,按照常规方法进行脱脂、脱钙、脱蛋白处理.设bDpDBM和bDBM微颗粒为研究对象,用于静态条件下骨髓基质干细胞(BMSCs)的培养,比较两组微颗粒对干细胞增殖及功能保持的影响,分析其用于BMSCs培养的可行性. 结果 两组微载体进行培养第14天后显示bDpDBM组细胞扩增较bDBM组快,差异有统计学意义(P＜0.05).经bDBM组培养的BMSCs的ALP定量及胞内钙含量明显较bDpDBM组多,差异有统计学意义(P＜0.05).而bDpDBM颗粒培养的BMSCs经诱导可向成骨、成脂及成软骨三系分化. 结论 bDpDBM由于去除了脱钙骨基质中的活性诱导成分,培养增殖效率增高,可较好地保持干细胞表型,适宜用作体外大量扩增BMSCs的微载体候选材料.
目的 比較牛去蛋白脫鈣皮質骨基質(bDpDBM)微顆粒和牛脫鈣皮質骨基質(bDBM)微顆粒用于體外榦細胞培養的優缺點,探討其作為微載體製備候選材料的可行性. 方法 冷凍粉碎牛四肢皮質骨,篩選齣直徑為200 ～ 500μm的微顆粒,按照常規方法進行脫脂、脫鈣、脫蛋白處理.設bDpDBM和bDBM微顆粒為研究對象,用于靜態條件下骨髓基質榦細胞(BMSCs)的培養,比較兩組微顆粒對榦細胞增殖及功能保持的影響,分析其用于BMSCs培養的可行性. 結果 兩組微載體進行培養第14天後顯示bDpDBM組細胞擴增較bDBM組快,差異有統計學意義(P＜0.05).經bDBM組培養的BMSCs的ALP定量及胞內鈣含量明顯較bDpDBM組多,差異有統計學意義(P＜0.05).而bDpDBM顆粒培養的BMSCs經誘導可嚮成骨、成脂及成軟骨三繫分化. 結論 bDpDBM由于去除瞭脫鈣骨基質中的活性誘導成分,培養增殖效率增高,可較好地保持榦細胞錶型,適宜用作體外大量擴增BMSCs的微載體候選材料.
목적 비교우거단백탈개피질골기질(bDpDBM)미과립화우탈개피질골기질(bDBM)미과립용우체외간세포배양적우결점,탐토기작위미재체제비후선재료적가행성. 방법 냉동분쇄우사지피질골,사선출직경위200 ～ 500μm적미과립,안조상규방법진행탈지、탈개、탈단백처리.설bDpDBM화bDBM미과립위연구대상,용우정태조건하골수기질간세포(BMSCs)적배양,비교량조미과립대간세포증식급공능보지적영향,분석기용우BMSCs배양적가행성. 결과 량조미재체진행배양제14천후현시bDpDBM조세포확증교bDBM조쾌,차이유통계학의의(P＜0.05).경bDBM조배양적BMSCs적ALP정량급포내개함량명현교bDpDBM조다,차이유통계학의의(P＜0.05).이bDpDBM과립배양적BMSCs경유도가향성골、성지급성연골삼계분화. 결론 bDpDBM유우거제료탈개골기질중적활성유도성분,배양증식효솔증고,가교호지보지간세포표형,괄의용작체외대량확증BMSCs적미재체후선재료.
Objective To discuss the potential of granular bovine deproteinized decalcified cortical bone matrix (bDpDBM) as a candidate for microcarriers by comparing bDpDBM and bovine decalcified cortical bone matrix (blDBM) in culture of bone marrow stromal stem cells (BMSCs) in vitro.Methods The cortical bone of bovine extremities was frozen and ground up to screen out granules of 200 ～500 μm in diameter.The tiny granules were degreased,decalcified and deproteinized in a conventional manner.bDpDBM and bDBM granules were used as scaffolds for static culture of BMSCs.The two groups were compared in terms of proliferation and maintenance of the multi-potentials of BMSCs.Results After 14 days' culture the proliferation of BMSCs in the bDpDBM group was significantly faster then in the bDBM group (P ＜ 0.05).The ALP expression and calcium deposition of BMSCs in the bDBM were significantly higher than in the bDpDBM group (P ＜ 0.05).The BMSCs cultured in the bDpDBM group could be induced into osteogenic,adipogenic anl chondrogenic differentiations,indicating high maintenance of multi-potentials of BMSCs.Conclusion Because bDpDBM granules may facilitate highly efficient culture of BMSCs and can preferably maintain the multi-potentials of BMSCs due to removal of components with inductive activity,they may be used as a good candidate microcarrier in cell culture in vitro.
