CAJ | 학술논문

目的探讨丝素纤维(SF)/磷酸钙骨水泥(CPC)新型复合材料修复兔桡骨骨缺损的效果.方法选取4个月龄新西兰大耳白兔48只制作兔双侧桡骨骨缺损模型,根据植入材料不同分为4组(n=12)∶SF/CPC组(植入SF/CPC新型复合材料)、SF/CPC颗粒骨组(植入SF/CPC新型复合材料和微小颗粒骨)、颗粒骨组(单纯植入微小颗粒骨)和空白对照组(不植入任何材料).术后给予相同饲养环境,并于术后4、8周时取出标本进行大体观察、X线检查、组织切片及扫描电镜观察,以及生物力学检测. 结果术后X线片示空白对照组骨缺损未修复,骨缺损断端硬化,而其他3组骨缺损均不同程度修复.根据影像学检查、组织学及扫描电镜,观察显示:术后4周SF/CPC组骨小梁形成较SF/CPC颗粒骨组和颗粒骨组均细小,术后8周3组骨小梁形成均粗大、成熟.术后4周生物力学检测显示SF/CPC组最大弯曲载荷分别与SF/CPC颗粒骨组和颗粒骨组比较,差异均有统计学意义(P＜0.05),而术后8周3组间两两比较差异均无统计学意义(P＞0.05).结论 SF/CPC新型复合材料有良好的生物相容性及骨诱导性,其力学强度与富含微小颗粒骨的复合材料相似,具备一定的骨缺损修复能力.
목적 탐토사소섬유(SF)/린산개골수니(CPC)신형복합재료수복토뇨골골결손적효과.방법 선취4개월령신서란대이백토48지제작토쌍측뇨골골결손모형,근거식입재료불동분위4조(n=12)∶SF/CPC조(식입SF/CPC신형복합재료)、SF/CPC과립골조(식입SF/CPC신형복합재료화미소과립골)、과립골조(단순식입미소과립골)화공백대조조(불식입임하재료).술후급여상동사양배경,병우술후4、8주시취출표본진행대체관찰、X선검사、조직절편급소묘전경관찰,이급생물역학검측. 결과 술후X선편시공백대조조골결손미수복,골결손단단경화,이기타3조골결손균불동정도수복.근거영상학검사、조직학급소묘전경,관찰현시:술후4주SF/CPC조골소량형성교SF/CPC과립골조화과립골조균세소,술후8주3조골소량형성균조대、성숙.술후4주생물역학검측현시SF/CPC조최대만곡재하분별여SF/CPC과립골조화과립골조비교,차이균유통계학의의(P＜0.05),이술후8주3조간량량비교차이균무통계학의의(P＞0.05).결론 SF/CPC신형복합재료유량호적생물상용성급골유도성,기역학강도여부함미소과립골적복합재료상사,구비일정적골결손수복능력.
Objective To explore the effects of silk fiber and calcium phosphate cement (SF/CPC),a new composite material,on repairing bone defects in rabbits.Methods Models of bilateral radial bone defects were created in 48 New Zealand big-eared white rabbits which were subsequently divided into 4 even groups according to the materials used to fill the defects:group A where SF/CPC was used,group B where SF/CPC plus micromorselized bone was used,group C where micromorselized bone was used alone,and group D where nothing was used.The rabbits were raised in the same conditions after operation.Samples were harvested at 4 and 8 weeks postoperation for gross observation,X-ray,histopathologic and scanning electron microscopy examinations; biomechanical tests were also conducted at 4 and 8 weeks postoperation for statistical analyses.Results X-ray examinations found no repair but sclerosis of bone ends in group D while different extents of bone repair were observed in the other 3 groups.According to the X-ray,histopathological and scanning electron microscopy examinations,trabecular bone formation in group A was thinner than in groups B and C at 4 weeks.Bulky and mature trabecular bone formation was observed in all groups at 8 weeks.Biomechanical tests showed group A had a significantly weaker strength than groups B and C at 4 weeks (P ＜ 0.05),but there were no significant differences among groups A,B and C at 8 weeks (P ＞ 0.05).Conclusion The new composite material SF/CPC has a certain capability of repairing bone defects,because it possesses biocompatibility and osteoinductive ability,and similar strength of micromorselized bone composite as well.