CAJ | 학술논문

目的探讨PKH26标记骨髓基质干细胞(BMSCs)经肋间动脉移植对家兔脊髓损伤(SCI)后神经功能恢复的影响. 方法采用动脉瘤夹法制备家兔T9节段SCI模型,建模成功后随机分为损伤对照组和BMSCs移植组.损伤后7d,损伤对照组仅造模,不行处理;BMSCs移植组经肋间动脉注射0.5mL标记PKH26的BMSCs.损伤前、后行BBB后肢运动功能评分和神经电生理检测,损伤后21d处死动物行脊髓组织病理学检查. 结果损伤后7d移植前两组BBB评分差异无统计学意义(P＞0.05),损伤后14、21 d BMSCs移植组BBB评分较损伤对照组高,差异有统计学意义(P＜0.05).损伤后14、21 d BMSCs移植组家兔的运动诱发电位(MEP)和感觉诱发电位(SEP)恢复较损伤对照组好,差异有统计学意义(P＜0.05).损伤后21 d在移植BMSCs家兔脊髓损伤区可发现PKH26标记的BMSCs阳性细胞聚集,苏木素-伊红染色显示两组脊髓损伤区均可见胶质瘢痕和空洞形成,但BMSCs移植组空洞面积小于损伤对照组;BMSCs移植组脊髓损伤组织内神经微丝200阳性表达强于损伤对照组,差异有统计学意义(P＜0.05). 结论 PKH26标记BMSCs经肋间动脉移植可有效促进家兔SCI后神经功能的恢复.
목적 탐토PKH26표기골수기질간세포(BMSCs)경륵간동맥이식대가토척수손상(SCI)후신경공능회복적영향. 방법 채용동맥류협법제비가토T9절단SCI모형,건모성공후수궤분위손상대조조화BMSCs이식조.손상후7d,손상대조조부조모,불행처리;BMSCs이식조경륵간동맥주사0.5mL표기PKH26적BMSCs.손상전、후행BBB후지운동공능평분화신경전생리검측,손상후21d처사동물행척수조직병이학검사. 결과 손상후7d이식전량조BBB평분차이무통계학의의(P＞0.05),손상후14、21 d BMSCs이식조BBB평분교손상대조조고,차이유통계학의의(P＜0.05).손상후14、21 d BMSCs이식조가토적운동유발전위(MEP)화감각유발전위(SEP)회복교손상대조조호,차이유통계학의의(P＜0.05).손상후21 d재이식BMSCs가토척수손상구가발현PKH26표기적BMSCs양성세포취집,소목소-이홍염색현시량조척수손상구균가견효질반흔화공동형성,단BMSCs이식조공동면적소우손상대조조;BMSCs이식조척수손상조직내신경미사200양성표체강우손상대조조,차이유통계학의의(P＜0.05). 결론 PKH26표기BMSCs경륵간동맥이식가유효촉진가토SCI후신경공능적회복.
Objective To investigate the effect of transplantation of PKH26-1abeled bone mesenchymal stem cells (BMSCs) via intercostal arteries on the neurological function recovery in rabbits with spinal cord injury (SCI).Methods The SCI models were made by aneurysm clips at the level of T9 segment in 20 rabbits.The successful rabbit SCI models were randomly divided into 2 equal groups.Seven days after SCI,the control group was not treated while in the transplantation group 0.5 mL of PKH26-1abeled BMSCs was injected through intercostal arteries.Basso-Beattie-Bresnahan (BBB) scoring and neuro-electrophysiology detection were conducted before and after SCI.The animals were sacrificed to harvest spinal cord specimens for histopathological examination 21 days after SCI.Results There were no significant differences between the 2 groups in BBB score 7 days after SCI (P ＞ 0.05).At 14 and 21 days after SCI,the BBB scores were significantly higher and recovery of motor evoked potentials (MEP) and somatosensory evoked potentials (SEP) was significantly better in the transplantation group than in the control group (P ＜ 0.05).PKH26-positive BMSCs gathered at the injured spinal sites 21 days after SCI in the transplantation group.Hematoxylin-Eosin (HE) staining showed visible glial scar tissue and cavity formation at the injured spinal sites in the 2 groups 21 days after SCI,but the cavity area in the transplantation group was smaller than in the control group.NF 200 positive expression was significantly enhanced in the transplantation group than in the control group (P ＜ 0.05).Conclusion Transplantation of PKH26-1abeled BMSCs via intercostal arteries can effectively promote the neurological function recovery in rabbits with spinal cord injury.