中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2013年
2期
128-134
,共7页
鼻窦炎%鼻黏膜%糖原合成酶激酶3%1-磷脂酰肌醇3-激酶%原癌基因蛋白质c-akt%白细胞介素6
鼻竇炎%鼻黏膜%糖原閤成酶激酶3%1-燐脂酰肌醇3-激酶%原癌基因蛋白質c-akt%白細胞介素6
비두염%비점막%당원합성매격매3%1-린지선기순3-격매%원암기인단백질c-akt%백세포개소6
Sinusitis%Nasal mucosa%Glycogen synthase kinase 3%1-phosphatidylinositol 3-kinase%Proto-oncogene proteins c-akt%Interleukin-6
目的 研究慢性鼻-鼻窦炎鼻黏膜中糖原合成酶激酶3β(glycogen synthase kinase 3β,GSK3β)与PI3K/Akt信号通路以及细胞因子白细胞介素(interleukin,IL)6之间的相关性及其意义.方法 分别用半定量反转录聚合酶链反应(RT-PCR)、免疫组化法检测30例慢性鼻-鼻窦炎伴鼻息肉(chronic rhinosinusitis with nasal polyps,CRSwNP)患者、20例慢性鼻-鼻窦炎不伴鼻息肉(chronic rhinosinusitis without nasal polyps,CRSsNP)患者的组织标本以及20例鼻中隔偏曲患者的鼻黏膜标本中GSK3β、PI3K、Akt的mRNA和蛋白质的表达,并用半定量RT-PCR检测细胞因子IL-6在CRSwNP组、CRSsNP组和对照组鼻黏膜中的表达.采用SPSS 16.0软件进行统计学分析.结果 GSK3β、PI3K、Akt、IL-6 mRNA在CRSwNP组中的相对表达量分别为0.6254±0.0584、0.8239±0.7186、0.9369±0.0823、0.8973±0.0680,在对照组分别为0.2684±0.0726、0.3578±0.0994、0.6721±0.0590、0.5898 ±0.0891,差异有统计学意义(t值分别为2.358、3.071、2.764、2.239,P值均＜0.05).以上四项指标在CRSwNP组和CRSsNP组之间的表达差异无统计学意义(t值分别为1.597、1.842、1.468、0.926,P值均＞0.05).GSK3β、PI3K、Akt蛋白主要表达在黏膜上皮细胞、黏膜下腺体细胞及炎性细胞中,阳性染色主要定位于胞质,其在CRS鼻黏膜中的阳性表达率明显高于对照组中的阳性表达率,差异有统计学意义(x2值分别为16.42、16.25、15.57,P值均＜0.01).GSK3β、PI3K、Akt蛋白在CRSwNP组和CRSsNP组的阳性表达率差异无统计学意义(x2值分别为3.27、2.85、2.46,P值均＞0.05).GSK3β与PI3K、Akt以及IL-6在CRS患者中的表达呈正相关趋势(r值分别为0.645、0.617、0.583,P值均＜0.01).结论 IL-6、PI3K、Akt、GSK3β在CRS鼻黏膜中的异常表达可能在CRS的发生、发展讨程中发挥着一定的作用.
目的 研究慢性鼻-鼻竇炎鼻黏膜中糖原閤成酶激酶3β(glycogen synthase kinase 3β,GSK3β)與PI3K/Akt信號通路以及細胞因子白細胞介素(interleukin,IL)6之間的相關性及其意義.方法 分彆用半定量反轉錄聚閤酶鏈反應(RT-PCR)、免疫組化法檢測30例慢性鼻-鼻竇炎伴鼻息肉(chronic rhinosinusitis with nasal polyps,CRSwNP)患者、20例慢性鼻-鼻竇炎不伴鼻息肉(chronic rhinosinusitis without nasal polyps,CRSsNP)患者的組織標本以及20例鼻中隔偏麯患者的鼻黏膜標本中GSK3β、PI3K、Akt的mRNA和蛋白質的錶達,併用半定量RT-PCR檢測細胞因子IL-6在CRSwNP組、CRSsNP組和對照組鼻黏膜中的錶達.採用SPSS 16.0軟件進行統計學分析.結果 GSK3β、PI3K、Akt、IL-6 mRNA在CRSwNP組中的相對錶達量分彆為0.6254±0.0584、0.8239±0.7186、0.9369±0.0823、0.8973±0.0680,在對照組分彆為0.2684±0.0726、0.3578±0.0994、0.6721±0.0590、0.5898 ±0.0891,差異有統計學意義(t值分彆為2.358、3.071、2.764、2.239,P值均＜0.05).以上四項指標在CRSwNP組和CRSsNP組之間的錶達差異無統計學意義(t值分彆為1.597、1.842、1.468、0.926,P值均＞0.05).GSK3β、PI3K、Akt蛋白主要錶達在黏膜上皮細胞、黏膜下腺體細胞及炎性細胞中,暘性染色主要定位于胞質,其在CRS鼻黏膜中的暘性錶達率明顯高于對照組中的暘性錶達率,差異有統計學意義(x2值分彆為16.42、16.25、15.57,P值均＜0.01).GSK3β、PI3K、Akt蛋白在CRSwNP組和CRSsNP組的暘性錶達率差異無統計學意義(x2值分彆為3.27、2.85、2.46,P值均＞0.05).GSK3β與PI3K、Akt以及IL-6在CRS患者中的錶達呈正相關趨勢(r值分彆為0.645、0.617、0.583,P值均＜0.01).結論 IL-6、PI3K、Akt、GSK3β在CRS鼻黏膜中的異常錶達可能在CRS的髮生、髮展討程中髮揮著一定的作用.
목적 연구만성비-비두염비점막중당원합성매격매3β(glycogen synthase kinase 3β,GSK3β)여PI3K/Akt신호통로이급세포인자백세포개소(interleukin,IL)6지간적상관성급기의의.방법 분별용반정량반전록취합매련반응(RT-PCR)、면역조화법검측30례만성비-비두염반비식육(chronic rhinosinusitis with nasal polyps,CRSwNP)환자、20례만성비-비두염불반비식육(chronic rhinosinusitis without nasal polyps,CRSsNP)환자적조직표본이급20례비중격편곡환자적비점막표본중GSK3β、PI3K、Akt적mRNA화단백질적표체,병용반정량RT-PCR검측세포인자IL-6재CRSwNP조、CRSsNP조화대조조비점막중적표체.채용SPSS 16.0연건진행통계학분석.결과 GSK3β、PI3K、Akt、IL-6 mRNA재CRSwNP조중적상대표체량분별위0.6254±0.0584、0.8239±0.7186、0.9369±0.0823、0.8973±0.0680,재대조조분별위0.2684±0.0726、0.3578±0.0994、0.6721±0.0590、0.5898 ±0.0891,차이유통계학의의(t치분별위2.358、3.071、2.764、2.239,P치균＜0.05).이상사항지표재CRSwNP조화CRSsNP조지간적표체차이무통계학의의(t치분별위1.597、1.842、1.468、0.926,P치균＞0.05).GSK3β、PI3K、Akt단백주요표체재점막상피세포、점막하선체세포급염성세포중,양성염색주요정위우포질,기재CRS비점막중적양성표체솔명현고우대조조중적양성표체솔,차이유통계학의의(x2치분별위16.42、16.25、15.57,P치균＜0.01).GSK3β、PI3K、Akt단백재CRSwNP조화CRSsNP조적양성표체솔차이무통계학의의(x2치분별위3.27、2.85、2.46,P치균＞0.05).GSK3β여PI3K、Akt이급IL-6재CRS환자중적표체정정상관추세(r치분별위0.645、0.617、0.583,P치균＜0.01).결론 IL-6、PI3K、Akt、GSK3β재CRS비점막중적이상표체가능재CRS적발생、발전토정중발휘착일정적작용.
Objective To explore the relationship among the expression of GSK3β,PI3K/Akt signaling transduction pathway,and cytokines IL-6 in chronic rhinosinusitis.Methods The mRNA and protein for GSK3β,PI3K,Akt were assayed by using reverse transcription polymerase chain reactoin(RTPCR)and immunohistochemistry(IHC),and the cytokines IL-6 mRNA measured by using reverse transcription polymerase chain reactoin(RT-PCR)in the nasal tissue from the patients with chronic rhinosinusitis with nasal polyps(CRSwNP),chronic rhinosinusitis without nasal polyps(CRSsNP)and control subjects.SPSS 16.0 software was used to analyze the data.Results The relative expression levels of GSK3β,PI3 K,Akt and IL-6 in CRSwNP were 0.6254 ± 0.0584,0.8239 ± 0.7186,0.9369 ± 0.0823 and0.8973 ±0.0680.But the relative expression levels of GSK3β,PI3K,Akt and IL-6 in control subjects were 0.2684 ± 0.0726,0.3578 ± 0.0994,0.6721 ± 0.0590,0.5898 ± 0.0891.There were significant differences between the groups of CRSwNP and control subjects(t values were 2.358,3.071,2.764,and 2.239,respectively,all P ＜ 0.05).There was no significant difference of GSK3β,PI3K,Akt and GSK3β between the groups of CRSwNP and CRSsNP(t values were 1.597,1.842,1.468 and 0.926,respectively,all P ＜ 0.05).GSK3β,PI3K,Akt were not only expressed in the cytoplasm of the epithelium and gland cells,but also in the cytoplasm of the inflammatory cell.GSK3β,PI3 K,Akt protein in CRS were deteced at a higher rate than the normal nasal tissue(x2 values were 16.42,16.25 and 15.57,respectively,all P ＜0.01).However there was no significant difference of GSK3β,PI3K,Akt protein between the groups of CRSwNP and CRSsNP(x2 values were 3.27,2.85 and 2.46,respectively,all P ＞ 0.05).There was a positive correlation trend among the expression of GSK3β,PI3K,Akt and IL-6 in CRS(r values were 0.645,0.617 and 0.583,respectively,all P ＜ 0.01).Conclusion The abnormal expression of IL-6,PI3K,Akt and GSK3β in the nasal mucosa of CRS may play a pro-inflammatory role in the occurrence and development of CRS.
