中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2013年
3期
224-228
,共5页
韩灵%孙悦奇%付清玲%文卫平%史剑波
韓靈%孫悅奇%付清玲%文衛平%史劍波
한령%손열기%부청령%문위평%사검파
疾病模型,动物%小鼠%呼吸超敏反应%卵白蛋白
疾病模型,動物%小鼠%呼吸超敏反應%卵白蛋白
질병모형,동물%소서%호흡초민반응%란백단백
Disease models,animal%Mice%Respiratory hypersensitivity%Ovalbumin
目的 探讨建立呼吸道变应性炎性反应小鼠模型的方法.方法 采用卵清白蛋白(ovalbumin,OVA)作为变应原,对模型组(5只)BALB/c小鼠腹腔注射OVA与免疫佐剂(氢氧化铝)致敏,空气雾化吸入联合鼻腔滴注OVA试剂激发的方法构建模型.另设对照组(5只),用磷酸盐缓冲液(PBS)替代OVA进行致敏和激发.末次激发后对小鼠鼻部症状进行评分,评估小鼠鼻黏膜嗜酸粒细胞浸润、肺组织支气管周围炎性反应细胞浸润和支气管上皮杯状细胞增生等病理学改变情况,检测小鼠血浆OVA特异性IgE抗体浓度,检测小鼠肺泡灌洗液中白细胞介素(interleukin,IL)4和IL-5细胞因子水平.结果 模型组小鼠能够成功模拟出打喷嚏、鼻痒挠鼻等症状,并且成功诱导出鼻中隔黏膜下嗜酸粒细胞及其他炎性反应细胞的浸润,肺部小支气管和小血管周围炎性反应细胞的浸润,以及支气管上皮杯状细胞的增生等病理生理学改变.模型组、对照组小鼠血浆OVA特异性IgE抗体质量浓度分别为(1237.00±153.20)、(191.90±43.20) pg/ml；模型组、对照组小鼠肺泡灌洗液中IL-4分别为(46.50±10.15)、(7.96±1.80)pg/ml,IL-5分别为(50.81±11.41)、(7.53±2.23) pg/ml,两组差异均有统计学意义(t值分别为6.569、3.738、3.724,P值均＜0.05).结论 采用OVA致敏和激发的造模方案,建立一种同时了解上下呼吸道的变应性炎性反应的小鼠模型,为研究发病机制和药效评估提供了一种可行的研究方法.
目的 探討建立呼吸道變應性炎性反應小鼠模型的方法.方法 採用卵清白蛋白(ovalbumin,OVA)作為變應原,對模型組(5隻)BALB/c小鼠腹腔註射OVA與免疫佐劑(氫氧化鋁)緻敏,空氣霧化吸入聯閤鼻腔滴註OVA試劑激髮的方法構建模型.另設對照組(5隻),用燐痠鹽緩遲液(PBS)替代OVA進行緻敏和激髮.末次激髮後對小鼠鼻部癥狀進行評分,評估小鼠鼻黏膜嗜痠粒細胞浸潤、肺組織支氣管週圍炎性反應細胞浸潤和支氣管上皮杯狀細胞增生等病理學改變情況,檢測小鼠血漿OVA特異性IgE抗體濃度,檢測小鼠肺泡灌洗液中白細胞介素(interleukin,IL)4和IL-5細胞因子水平.結果 模型組小鼠能夠成功模擬齣打噴嚏、鼻癢撓鼻等癥狀,併且成功誘導齣鼻中隔黏膜下嗜痠粒細胞及其他炎性反應細胞的浸潤,肺部小支氣管和小血管週圍炎性反應細胞的浸潤,以及支氣管上皮杯狀細胞的增生等病理生理學改變.模型組、對照組小鼠血漿OVA特異性IgE抗體質量濃度分彆為(1237.00±153.20)、(191.90±43.20) pg/ml；模型組、對照組小鼠肺泡灌洗液中IL-4分彆為(46.50±10.15)、(7.96±1.80)pg/ml,IL-5分彆為(50.81±11.41)、(7.53±2.23) pg/ml,兩組差異均有統計學意義(t值分彆為6.569、3.738、3.724,P值均＜0.05).結論 採用OVA緻敏和激髮的造模方案,建立一種同時瞭解上下呼吸道的變應性炎性反應的小鼠模型,為研究髮病機製和藥效評估提供瞭一種可行的研究方法.
목적 탐토건립호흡도변응성염성반응소서모형적방법.방법 채용란청백단백(ovalbumin,OVA)작위변응원,대모형조(5지)BALB/c소서복강주사OVA여면역좌제(경양화려)치민,공기무화흡입연합비강적주OVA시제격발적방법구건모형.령설대조조(5지),용린산염완충액(PBS)체대OVA진행치민화격발.말차격발후대소서비부증상진행평분,평고소서비점막기산립세포침윤、폐조직지기관주위염성반응세포침윤화지기관상피배상세포증생등병이학개변정황,검측소서혈장OVA특이성IgE항체농도,검측소서폐포관세액중백세포개소(interleukin,IL)4화IL-5세포인자수평.결과 모형조소서능구성공모의출타분체、비양뇨비등증상,병차성공유도출비중격점막하기산립세포급기타염성반응세포적침윤,폐부소지기관화소혈관주위염성반응세포적침윤,이급지기관상피배상세포적증생등병리생이학개변.모형조、대조조소서혈장OVA특이성IgE항체질량농도분별위(1237.00±153.20)、(191.90±43.20) pg/ml；모형조、대조조소서폐포관세액중IL-4분별위(46.50±10.15)、(7.96±1.80)pg/ml,IL-5분별위(50.81±11.41)、(7.53±2.23) pg/ml,량조차이균유통계학의의(t치분별위6.569、3.738、3.724,P치균＜0.05).결론 채용OVA치민화격발적조모방안,건립일충동시료해상하호흡도적변응성염성반응적소서모형,위연구발병궤제화약효평고제공료일충가행적연구방법.
Objective To investigate the method of development of allergic airway disease model in mice.Methods Ten BALB/c mice were devided into the model group and the control group.Each group contained 5 mice.Ovalbumin (OVA) was used as allergen.OVA was emulsified with aluminum hydroxide and injected intraperitoneally for sensitization.Afterwards the mice from model group were challenged with aerosolized 5％ OVA and subsequently instilled with OVA intranasally.For the blank control group the mice were sensitized and challenged with phosphate buffer saline (PBS).After final challenge,the nasal symptoms were scored,and mice were sacrificed for evaluation of eosinophilia of nasal septum,peribronchial inflammation and goblet cell hyperplasia.Mice serum was collected for measurement of OVA-specific IgE concentration,and levels of IL-4 and IL-5 from bronchoalveolar fluids were also tested.Results Compared with blank control mice,mice from model group displayed typical sneezing and nasal scratching symptoms.The histopathological changes,such as eosinophilia of nasal septum mucosa,infiltration of peribronchial inflammatory cells and hyperplasia of goblet cells were successfully induced by OVA sensitization and challenge.Moreover,mice in model group showed higher level of OVA-specific IgE in serum and IL-4 and IL-5 cytokines in bronchoalveolar fluids[mice from model group:IgE (1237.00 ± 153.20) pg/ml,IL-4 (46.50 ± 10.15) pg/ml,IL-5 (50.81 ± 11.41) pg/ml; mice from control group:IgE (191.90 ±43.20)pg/ml,IL-4 (7.96 ±1.80) pg/ml,IL-5 (7.53 ±2.23) pg/ml;t value were 6.569,3.738 and 3.724,respectively,all P ＜ 0.05].Conclusion The method using OVA as allergen could effectively develop a mouse model of allergic airway disease which could be used for pathogenesis study and drug effect evaluation.
