中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2013年
4期
311-315
,共5页
李文婷%张革化%黎景佳%常利红%王凯%杨钦泰
李文婷%張革化%黎景佳%常利紅%王凱%楊欽泰
리문정%장혁화%려경가%상리홍%왕개%양흠태
受体,抗原,T细胞,γ-δ%鼻窦炎%鼻息肉%鼻黏膜%嗜酸细胞
受體,抗原,T細胞,γ-δ%鼻竇炎%鼻息肉%鼻黏膜%嗜痠細胞
수체,항원,T세포,γ-δ%비두염%비식육%비점막%기산세포
Receptors,antigen,T-cell,gamma-delta%Sinusitis%Nasal ployps%Nasal mucosa%Eosinophils
目的 观察γδ T细胞在慢性鼻-鼻窦炎(chronic rhinosinusitis,CRS)患者鼻黏膜组织中的表达,探讨其在CRS发病机制中的意义.方法 免疫组化(Envision二步法)分别检测CRS伴鼻息肉(chronic rhinosinusitis with nasal polyps,CRSwNP)患者息肉组织(25例)、CRS不伴鼻息肉(chronic rhinosinusitis without nasal polyps,CRSsNP)患者下鼻甲黏膜(13例)及对照组鼻中隔偏曲患者下鼻甲黏膜(16例)中γδ T细胞的表达情况；HE染色观察嗜酸粒细胞浸润型CRSwNP中嗜酸粒细胞浸润程度.比较各组间γδ T细胞表达差异,分析嗜酸粒细胞浸润型CRSwNP中γδ T细胞表达与嗜酸粒细胞浸润程度的相关性.采用SPSS 16.0软件对数据进行分析.结果 CRSwNP组和CRSsNP组中yδT细胞阳性率分别为88.0％、84.6％,均高于对照组的37.5％,差异有统计学意义(x2=13.413,P＜0.01；x2=6.564,P＜0.05).CRSwNP组与CRSsNP组相比,γδ T细胞阳性率差异无统计学意义(x2=0.086,P＞0.05).CRSwNP组中γδ T细胞表达程度高于CRSsNP组和对照组(U值分别为596、296,P值均＜0.01),CRSsNP组γδ T细胞表达程度高于对照组(U=216,P＜0.05).CRSwNP组中γδT细胞表达与嗜酸粒细胞浸润程度呈正相关趋势(r=0.579,P＜0.05).结论 γδ T细胞在CRS中表达增高,并可能促进鼻黏膜组织中嗜酸粒细胞浸润,可能在CRS发病机制中发挥作用.
目的 觀察γδ T細胞在慢性鼻-鼻竇炎(chronic rhinosinusitis,CRS)患者鼻黏膜組織中的錶達,探討其在CRS髮病機製中的意義.方法 免疫組化(Envision二步法)分彆檢測CRS伴鼻息肉(chronic rhinosinusitis with nasal polyps,CRSwNP)患者息肉組織(25例)、CRS不伴鼻息肉(chronic rhinosinusitis without nasal polyps,CRSsNP)患者下鼻甲黏膜(13例)及對照組鼻中隔偏麯患者下鼻甲黏膜(16例)中γδ T細胞的錶達情況；HE染色觀察嗜痠粒細胞浸潤型CRSwNP中嗜痠粒細胞浸潤程度.比較各組間γδ T細胞錶達差異,分析嗜痠粒細胞浸潤型CRSwNP中γδ T細胞錶達與嗜痠粒細胞浸潤程度的相關性.採用SPSS 16.0軟件對數據進行分析.結果 CRSwNP組和CRSsNP組中yδT細胞暘性率分彆為88.0％、84.6％,均高于對照組的37.5％,差異有統計學意義(x2=13.413,P＜0.01；x2=6.564,P＜0.05).CRSwNP組與CRSsNP組相比,γδ T細胞暘性率差異無統計學意義(x2=0.086,P＞0.05).CRSwNP組中γδ T細胞錶達程度高于CRSsNP組和對照組(U值分彆為596、296,P值均＜0.01),CRSsNP組γδ T細胞錶達程度高于對照組(U=216,P＜0.05).CRSwNP組中γδT細胞錶達與嗜痠粒細胞浸潤程度呈正相關趨勢(r=0.579,P＜0.05).結論 γδ T細胞在CRS中錶達增高,併可能促進鼻黏膜組織中嗜痠粒細胞浸潤,可能在CRS髮病機製中髮揮作用.
목적 관찰γδ T세포재만성비-비두염(chronic rhinosinusitis,CRS)환자비점막조직중적표체,탐토기재CRS발병궤제중적의의.방법 면역조화(Envision이보법)분별검측CRS반비식육(chronic rhinosinusitis with nasal polyps,CRSwNP)환자식육조직(25례)、CRS불반비식육(chronic rhinosinusitis without nasal polyps,CRSsNP)환자하비갑점막(13례)급대조조비중격편곡환자하비갑점막(16례)중γδ T세포적표체정황；HE염색관찰기산립세포침윤형CRSwNP중기산립세포침윤정도.비교각조간γδ T세포표체차이,분석기산립세포침윤형CRSwNP중γδ T세포표체여기산립세포침윤정도적상관성.채용SPSS 16.0연건대수거진행분석.결과 CRSwNP조화CRSsNP조중yδT세포양성솔분별위88.0％、84.6％,균고우대조조적37.5％,차이유통계학의의(x2=13.413,P＜0.01；x2=6.564,P＜0.05).CRSwNP조여CRSsNP조상비,γδ T세포양성솔차이무통계학의의(x2=0.086,P＞0.05).CRSwNP조중γδ T세포표체정도고우CRSsNP조화대조조(U치분별위596、296,P치균＜0.01),CRSsNP조γδ T세포표체정도고우대조조(U=216,P＜0.05).CRSwNP조중γδT세포표체여기산립세포침윤정도정정상관추세(r=0.579,P＜0.05).결론 γδ T세포재CRS중표체증고,병가능촉진비점막조직중기산립세포침윤,가능재CRS발병궤제중발휘작용.
Objective To explore the expression of γδ T cells in chronic rhinosinusitis (CRS) and its potential significance in pathogenesis.Methods γδ T cell expression was detected by immunohistochemistry (Envision method).From polyps (25 CRS patients with nasal polyps,CRSwNP),inferior turbinate mucosa (13 CRS patients without nasal polyps,CRSsNP),and 16 inferior turbinate mucosa from patients with deviation of nasal septum served as control.The infiltration of eosinophils in eosinophilic CRSwNP was observed by HE staining.The differences of expression of γδ T cells between each groups were compared,meanwhile the relationship between γδ T cells and eosinophils were analyzed.SPSS 16.0 software was used to analyze the data.Results The positive range of γδ T cells in CRSwNP group and CRSsNP group was 88.0％ and 84.6％,respectively,both higher than 37.5％ in control group (x2 =13.413,P ＜ 0.01,x2 =6.564,P ＜ 0.05,respectively),CRSwNP group had no statistical significance compared with CRSsNP group(x2=0.086,P ＞0.05).The expression of γδ T cells in CRSwNP group was stronger than CRSsNP group and control group (U =596,P ＜ 0.01,U =296,P ＜ 0.01,respectively) ; CRSsNP group was stronger than control group(U =216,P ＜ 0.05).There was a positive correlation between γδ T cells and eosinophils (r =0.579,P ＜ 0.05).Conclusions The expression of γδ T cells was increased in nasal mucosa of CRS.γδ T cells may be involved in the pathogenesis of CRS.
