CAJ | 학술논문

目的探讨声门上型喉鳞状细胞癌(简称鳞癌)颈淋巴转移中上皮-间质转化过程相关的小分子RNA(microRNA,miRNA).方法对12例声门上型喉鳞癌患者(淋巴转移组6例,非淋巴转移组6例)的原发肿瘤组织进行miRNA芯片筛选；对40例声门上型喉鳞癌患者(淋巴转移组20例,非淋巴转移组20例)通过实时定量PCR法验证筛选出的显著差异表达的miRNA；应用生物信息学预测已验证的miRNA的靶基因,根据各基因的功能筛选与上皮-间质转化相关的基因,对应找到可能与声门上型喉鳞癌颈淋巴转移中上皮-间质转化过程相关的miRNA.结果芯片筛选结果显示10个miRNA在两组间的表达差异有意义,其中在6例转移组中上调的有miR-192、miR-634、miR-223、miR-409、miR-365、miR-143、miR-1224、miR-30d和miR-1249,下调的有miR-494.40例较大样本验证结果与芯片筛选结果相符.通过靶基因预测及功能分析,miR-192、miR-143、miR-409及miR-634可能通过抑制Krüppel样转录因子、E-钙黏蛋白和磷脂酰肌醇3-激酶等靶基因的转录上调上皮-间质转化过程,从而促进声门上型喉鳞癌颈淋巴转移.结论筛选和验证与声门上型喉鳞癌颈淋巴转移相关的miRNA,miR-192、miR-143、miR-409及miR-634的表达失调可能在声门上型喉鳞癌转移的上皮-间质转化过程中扮演着重要的角色,需要进一步深入研究.
목적 탐토성문상형후린상세포암(간칭린암)경림파전이중상피-간질전화과정상관적소분자RNA(microRNA,miRNA).방법 대12례성문상형후린암환자(림파전이조6례,비림파전이조6례)적원발종류조직진행miRNA심편사선；대40례성문상형후린암환자(림파전이조20례,비림파전이조20례)통과실시정량PCR법험증사선출적현저차이표체적miRNA；응용생물신식학예측이험증적miRNA적파기인,근거각기인적공능사선여상피-간질전화상관적기인,대응조도가능여성문상형후린암경림파전이중상피-간질전화과정상관적miRNA.결과 심편사선결과현시10개miRNA재량조간적표체차이유의의,기중재6례전이조중상조적유miR-192、miR-634、miR-223、miR-409、miR-365、miR-143、miR-1224、miR-30d화miR-1249,하조적유miR-494.40례교대양본험증결과여심편사선결과상부.통과파기인예측급공능분석,miR-192、miR-143、miR-409급miR-634가능통과억제Krüppel양전록인자、E-개점단백화린지선기순3-격매등파기인적전록상조상피-간질전화과정,종이촉진성문상형후린암경림파전이.결론 사선화험증여성문상형후린암경림파전이상관적miRNA,miR-192、miR-143、miR-409급miR-634적표체실조가능재성문상형후린암전이적상피-간질전화과정중분연착중요적각색,수요진일보심입연구.
Objective To study microRNAs (miRNAs) expression profiles associated with epithelial-mesenchymal transition (EMT) in lymph node metastasis of supraglottic laryngeal squamous cell carcinomas(SGLSCC).Methods Primary tumor tissue samples of 12 SGLSCC patients were collected,including 6 patients clinically diagnosed with lymph nodes metastasis (N +) and 6 patients with lymph nodes metastasis-free (N0),for miRNA microarray gene-expression profiling to identify the differences between N+ and NO groups.Differentially expressed miRNAs was verified using quantitative real-time PCR in 20 patients with N + and 20 patients with NO.Target genes for the miRNAs associated with EMT in SGLSCC metastasis were analyzed.Results Ten miRNAs differentially expressed between N + group and NO group were determined.Comparing with NO group,nine miRNAs were over-expressed and one miRNA was expressed at lower level in N+ group.The genes for miR-192,miR-143,miR-409 and miR-634 were predicted as target genes that could promote EMT of laryngeal cancer cells by targeted inhibiting Krüppel-like factor 17(KLF17),E-cadherin and phosphatidylinositol 3 kinase (PI3K).Conclusions The miRNAs over-expressed in group N + can be used to predict cervical lymph node metastasis in SGLSCC.The miRNAs as new markers could improve the diagnosis and treatment of SGLSCC.