中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2013年
7期
544-548
,共5页
黄雪琨%杨钦泰%陈玉莲%张富程%张革化
黃雪琨%楊欽泰%陳玉蓮%張富程%張革化
황설곤%양흠태%진옥련%장부정%장혁화
受体,抗原,T细胞,γ-δ%T淋巴细胞,调节性%白细胞介素17%转化生长因子β1%鼻炎,变应性,常年性%鼻炎,变应性,季节性
受體,抗原,T細胞,γ-δ%T淋巴細胞,調節性%白細胞介素17%轉化生長因子β1%鼻炎,變應性,常年性%鼻炎,變應性,季節性
수체,항원,T세포,γ-δ%T림파세포,조절성%백세포개소17%전화생장인자β1%비염,변응성,상년성%비염,변응성,계절성
Receptors,antigen,T-cell,gamma-delta%T-lymphocytes,regulatory%Interleukin-17%Transforming growth factor beta 1%Rhinitis,allergic,perennial%Rhinitis,allergic,seasonal
目的 探讨变应性鼻炎(allergic rhinitis,AR)患者外周血中γδ T细胞、调节性T细胞(regulatory T cells,Treg)、白细胞介素(interleukin,IL) 17及转化生长因子β1(transforming growth factor β1,TGF-β1)的表达及临床意义.方法 2012年3-7月,收集32例AR患者(AR组)和20例健康志愿者(对照组)的外周血,采用流式细胞术检测外周血中γδ T细胞和Treg细胞比例；酶联免疫吸附试验检测血清中IL-17、TGF-β1的水平,并分析它们之间的相关性.采用SPSS 16.0软件进行数据分析.结果 γδ T细胞百分比在AR组和对照组分别为(13.30±8.62)％、(5.18±1.86)％,差异有统计学意义(t =5.18,P＜0.01)；Treg细胞百分比分别为(1.75±0.56)％、(4.76±1.74)％,差异有统计学意义(t =7.46,P＜0.01).AR组血清中IL-17的表达水平为(668.55 ±45.15) pg/ml,高于对照组的(573.53±17.42) pg/ml,差异有统计学意义(t=8.97,P＜0.01)；AR组TGF-β1的表达水平为(0.34±0.04) pg/ml,低于对照组的(0.49 ±0.06) pg/ml,差异有统计学意义(t=9.51,P＜0.01).AR组患者外周血中γδ T细胞百分比与Treg细胞百分比呈负相关趋势(r=-0.561,P＜0.01),γδ T细胞百分比与IL-17呈正相关趋势(r=0.469,P＜0.01),γδ T细胞百分比与TGF-β1呈负相关趋势(r=-0.622,P＜0.01),Treg细胞百分比与TGF-β1呈正相关(r=0.738,P＜0.01),IL-17表达水平与Treg细胞百分比、TGF-β1无相关性(r值分别为-0.111、-0.196,P＞0.05).结论 AR患者外周血中γδ T细胞和Treg细胞之间存在免疫平衡失调,γδ T细胞可能参与了IL-17的分泌,这可能在AR的发病和发展过程中发挥重要作用.
目的 探討變應性鼻炎(allergic rhinitis,AR)患者外週血中γδ T細胞、調節性T細胞(regulatory T cells,Treg)、白細胞介素(interleukin,IL) 17及轉化生長因子β1(transforming growth factor β1,TGF-β1)的錶達及臨床意義.方法 2012年3-7月,收集32例AR患者(AR組)和20例健康誌願者(對照組)的外週血,採用流式細胞術檢測外週血中γδ T細胞和Treg細胞比例；酶聯免疫吸附試驗檢測血清中IL-17、TGF-β1的水平,併分析它們之間的相關性.採用SPSS 16.0軟件進行數據分析.結果 γδ T細胞百分比在AR組和對照組分彆為(13.30±8.62)％、(5.18±1.86)％,差異有統計學意義(t =5.18,P＜0.01)；Treg細胞百分比分彆為(1.75±0.56)％、(4.76±1.74)％,差異有統計學意義(t =7.46,P＜0.01).AR組血清中IL-17的錶達水平為(668.55 ±45.15) pg/ml,高于對照組的(573.53±17.42) pg/ml,差異有統計學意義(t=8.97,P＜0.01)；AR組TGF-β1的錶達水平為(0.34±0.04) pg/ml,低于對照組的(0.49 ±0.06) pg/ml,差異有統計學意義(t=9.51,P＜0.01).AR組患者外週血中γδ T細胞百分比與Treg細胞百分比呈負相關趨勢(r=-0.561,P＜0.01),γδ T細胞百分比與IL-17呈正相關趨勢(r=0.469,P＜0.01),γδ T細胞百分比與TGF-β1呈負相關趨勢(r=-0.622,P＜0.01),Treg細胞百分比與TGF-β1呈正相關(r=0.738,P＜0.01),IL-17錶達水平與Treg細胞百分比、TGF-β1無相關性(r值分彆為-0.111、-0.196,P＞0.05).結論 AR患者外週血中γδ T細胞和Treg細胞之間存在免疫平衡失調,γδ T細胞可能參與瞭IL-17的分泌,這可能在AR的髮病和髮展過程中髮揮重要作用.
목적 탐토변응성비염(allergic rhinitis,AR)환자외주혈중γδ T세포、조절성T세포(regulatory T cells,Treg)、백세포개소(interleukin,IL) 17급전화생장인자β1(transforming growth factor β1,TGF-β1)적표체급림상의의.방법 2012년3-7월,수집32례AR환자(AR조)화20례건강지원자(대조조)적외주혈,채용류식세포술검측외주혈중γδ T세포화Treg세포비례；매련면역흡부시험검측혈청중IL-17、TGF-β1적수평,병분석타문지간적상관성.채용SPSS 16.0연건진행수거분석.결과 γδ T세포백분비재AR조화대조조분별위(13.30±8.62)％、(5.18±1.86)％,차이유통계학의의(t =5.18,P＜0.01)；Treg세포백분비분별위(1.75±0.56)％、(4.76±1.74)％,차이유통계학의의(t =7.46,P＜0.01).AR조혈청중IL-17적표체수평위(668.55 ±45.15) pg/ml,고우대조조적(573.53±17.42) pg/ml,차이유통계학의의(t=8.97,P＜0.01)；AR조TGF-β1적표체수평위(0.34±0.04) pg/ml,저우대조조적(0.49 ±0.06) pg/ml,차이유통계학의의(t=9.51,P＜0.01).AR조환자외주혈중γδ T세포백분비여Treg세포백분비정부상관추세(r=-0.561,P＜0.01),γδ T세포백분비여IL-17정정상관추세(r=0.469,P＜0.01),γδ T세포백분비여TGF-β1정부상관추세(r=-0.622,P＜0.01),Treg세포백분비여TGF-β1정정상관(r=0.738,P＜0.01),IL-17표체수평여Treg세포백분비、TGF-β1무상관성(r치분별위-0.111、-0.196,P＞0.05).결론 AR환자외주혈중γδ T세포화Treg세포지간존재면역평형실조,γδ T세포가능삼여료IL-17적분비,저가능재AR적발병화발전과정중발휘중요작용.
Objective To investigate the expression of peripheral blood γδ T cells/CD4 + CD25 + regulatory T cells(Treg) and cytokines interleukin 17 (IL-17) and transforming growth factor β1 (TGF-β1) in patients with allergic rhinitis.Methods From March 2012 to July 2012,32 patients with allergic rhinitis (AR group) and 20 healthy control subjects (control group) were collected.The expression of peripheral blood γδ T cells/Treg cells were measured by flow cytometry and the levels of IL-17 and TGF-β1 were evaluated by ELISA.SPSS 16.0 software was used to analyze the data.Results The percentages of γδT cells in AR group were (13.30 ±8.62)％,which was significantly higher (t =5.18,P ＜0.01) than those in control group (5.18 ± 1.86)％.The percentages of Treg cells in AR group were (1.75 ±0.56)％,which were significantly lower (t =7.46,P ＜ 0.01) than those in control group (4.76 ± 1.74) ％.The IL-17 levels in AR group were (668.55 ±45.15) pg/ml,which were also significantly higher (t =8.97,P ＜ 0.01) than those in control group (573.53 ± 17.42) pg/ml.The TGF-β1 levels in AR group were (0.34 ±0.04) pg/ml,which were also significantly lower (t =9.51,P ＜0.01) than those in control group(0.49 ± 0.06) pg/ml.There was a negative correlation between the percentages of γδ T cells and Treg cells (r =-0.561,P ＜0.01).There was a negative correlation between the percentages of γδ T cells and TGF-β1 levels (r =-0.622,P ＜ 0.01).A positive correlation was shown between the percentages of βδ T cells and IL-17 levels in AR (r =0.469,P ＜ 0.01).A positive correlation was shown between the percentages of Treg cells and TGF-β1 levels in AR (r =0.738,P ＜0.01).There was no correlation between IL-17 levels and the percentages of Treg cells or TGF-β1 levels (r value was-0.111,-0.196,all P ＞ 0.05).Conclusion There are imbalances of γδ T and Treg cells in peripheral blood of patients with allergic rhinitis.γδ T cells may be the main cell to produce IL-17,which may play an important role in allergic rhinitis.
