中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2013年
8期
673-676
,共4页
X线显微体层摄影术%耳%成像,三维%小鼠
X線顯微體層攝影術%耳%成像,三維%小鼠
X선현미체층섭영술%이%성상,삼유%소서
X-ray microtomography%Ear%Imaging,three-dimensional%Mice
目的 应用基于相位衬度成像技术的显微CT(Micro-CT)对小鼠听泡进行形态学观察,建立中耳、内耳的3D模型,探讨Micro-CT在耳科影像学上的应用.方法 颈椎脱臼处死小鼠,剥离听泡,苦味酸固定后行Micro-CT扫描,获得2D图像,应用Amira-5.4建模重构获得3D模型.结果 获得了空间视觉良好的3D模型,鼓膜、中耳听骨链及其局部结构清晰,内耳前庭、半规管、耳蜗形态完整,内部微结构显像；听泡模型整体立体透视良好,可做任意的轴向旋转,得到所需目标图像.结论 应用Micro-CT对小鼠听泡进行无损成像,可同时获得标本的外部形貌和内部细节特征,所获图像分辨率高,建模重构所获3D模型空间视觉较好.Micro-CT样本制备过程简单,操作便捷,设备占用空间小,具有良好的发展前景.
目的 應用基于相位襯度成像技術的顯微CT(Micro-CT)對小鼠聽泡進行形態學觀察,建立中耳、內耳的3D模型,探討Micro-CT在耳科影像學上的應用.方法 頸椎脫臼處死小鼠,剝離聽泡,苦味痠固定後行Micro-CT掃描,穫得2D圖像,應用Amira-5.4建模重構穫得3D模型.結果 穫得瞭空間視覺良好的3D模型,鼓膜、中耳聽骨鏈及其跼部結構清晰,內耳前庭、半規管、耳蝸形態完整,內部微結構顯像；聽泡模型整體立體透視良好,可做任意的軸嚮鏇轉,得到所需目標圖像.結論 應用Micro-CT對小鼠聽泡進行無損成像,可同時穫得標本的外部形貌和內部細節特徵,所穫圖像分辨率高,建模重構所穫3D模型空間視覺較好.Micro-CT樣本製備過程簡單,操作便捷,設備佔用空間小,具有良好的髮展前景.
목적 응용기우상위츤도성상기술적현미CT(Micro-CT)대소서은포진행형태학관찰,건립중이、내이적3D모형,탐토Micro-CT재이과영상학상적응용.방법 경추탈구처사소서,박리은포,고미산고정후행Micro-CT소묘,획득2D도상,응용Amira-5.4건모중구획득3D모형.결과 획득료공간시각량호적3D모형,고막、중이은골련급기국부결구청석,내이전정、반규관、이와형태완정,내부미결구현상；은포모형정체입체투시량호,가주임의적축향선전,득도소수목표도상.결론 응용Micro-CT대소서은포진행무손성상,가동시획득표본적외부형모화내부세절특정,소획도상분변솔고,건모중구소획3D모형공간시각교호.Micro-CT양본제비과정간단,조작편첩,설비점용공간소,구유량호적발전전경.
Objective To get the three-Dimensional imaging model of internal ear and middle ear using the Micro-CT equipment,and research the nondestructive imaging of mice acoustic capsule,and investigate the application of Micro-CT inmorphology of acoustic capsule.Methods The mice were executed to harvest the acoustic capsules.The acoustic capsules were fixed in trinitrophenol before scanning by Micro-CT.Using this equipment,we obtained a 3D model of reconstruction from 2D images.Results The 3D model of acoustic capsule and the model was extraordinary clear to show the eardrum,ossicles chain,vestibulum,semicircular canal,and cochlear.The 3D structure model was excellent for look through,and it was free to revolve in any axial direction on the platform of software Amira-5.4.Conclusions By using Micro-CT equipment to research the nondestructive three-Dimensional imaging of acoustic capsule,the outside structures and internal details of the acoustic capsule can be observed at the same time.3D structure model which was good for looking through.Using Micro-CT equipment is easy to operate and also can save a lot of samples,and the images have high resolution ratio.Micro-CT has good prospects for development.
