中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2013年
12期
1017-1021
,共5页
周健%陶磊%张铎%陈斌%汤玮晶%路丽明
週健%陶磊%張鐸%陳斌%湯瑋晶%路麗明
주건%도뢰%장탁%진빈%탕위정%로려명
声带%白斑,口腔%白细胞介素10%多态性,单核苷酸%疾病遗传易感性%癌前状态
聲帶%白斑,口腔%白細胞介素10%多態性,單覈苷痠%疾病遺傳易感性%癌前狀態
성대%백반,구강%백세포개소10%다태성,단핵감산%질병유전역감성%암전상태
Vocal cords%Leukoplakia,oral%Interleukin-10%Polymorphism,single nucleotide%Genetic predisposition to diseases%Precancerous conditions
目的 探讨白细胞介素10(IL-10)启动子区-1082/-819/-592位点的多态性及其在血浆中的浓度与声带白斑发生的关系.方法 选择2012年10月至2013年2月61例声带白斑患者和119名正常人作对照研究,采用核苷酸直接测序法检测样本的IL-10基因位点(-1082G/A,-819C/T和-592C/A)的基因分型,用酶联免疫吸附法(ELISA)检测IL-10的血浆浓度.结果 吸烟和饮酒增加了罹患声带白斑的风险(OR为4.73和5.70,95％ CI为2.4 ～9.1;2.9～11.2,P＜0.01).声带白斑患者在IL-10的-592和-819位点比对照组有更高的AC突变频率(OR=1.93,95％ CI为0.97～ 3.81,P=0.05),在-1082位点有更高的AG突变频率(OR =2.14,95％ CI为0.87 ～5.27,P=0.092);声带白斑患者的血浆IL-10质量浓度为(21.6 ±0.5)pg/ml((x)±s),对照组为(19.0±1.1) pg/ml,两者比较差异有统计学意义(t=2.08,P=0.043).基因型中含有G基因的单倍体患者的血浆IL-10质量浓度(24.3±5.7)pg/ml,比正常基因型单倍体患者IL-10的浓度高(19.9 ±4.7) pg/ml,两者比较差异有统计学意义(t=-2.64,P=0.008).结论 IL-10启动子区-1082/-819/-592多态性及血浆中高浓度的IL-10与声带白斑的发生有关,同时,突变基因型与IL-10血浆浓度有关.
目的 探討白細胞介素10(IL-10)啟動子區-1082/-819/-592位點的多態性及其在血漿中的濃度與聲帶白斑髮生的關繫.方法 選擇2012年10月至2013年2月61例聲帶白斑患者和119名正常人作對照研究,採用覈苷痠直接測序法檢測樣本的IL-10基因位點(-1082G/A,-819C/T和-592C/A)的基因分型,用酶聯免疫吸附法(ELISA)檢測IL-10的血漿濃度.結果 吸煙和飲酒增加瞭罹患聲帶白斑的風險(OR為4.73和5.70,95％ CI為2.4 ～9.1;2.9～11.2,P＜0.01).聲帶白斑患者在IL-10的-592和-819位點比對照組有更高的AC突變頻率(OR=1.93,95％ CI為0.97～ 3.81,P=0.05),在-1082位點有更高的AG突變頻率(OR =2.14,95％ CI為0.87 ～5.27,P=0.092);聲帶白斑患者的血漿IL-10質量濃度為(21.6 ±0.5)pg/ml((x)±s),對照組為(19.0±1.1) pg/ml,兩者比較差異有統計學意義(t=2.08,P=0.043).基因型中含有G基因的單倍體患者的血漿IL-10質量濃度(24.3±5.7)pg/ml,比正常基因型單倍體患者IL-10的濃度高(19.9 ±4.7) pg/ml,兩者比較差異有統計學意義(t=-2.64,P=0.008).結論 IL-10啟動子區-1082/-819/-592多態性及血漿中高濃度的IL-10與聲帶白斑的髮生有關,同時,突變基因型與IL-10血漿濃度有關.
목적 탐토백세포개소10(IL-10)계동자구-1082/-819/-592위점적다태성급기재혈장중적농도여성대백반발생적관계.방법 선택2012년10월지2013년2월61례성대백반환자화119명정상인작대조연구,채용핵감산직접측서법검측양본적IL-10기인위점(-1082G/A,-819C/T화-592C/A)적기인분형,용매련면역흡부법(ELISA)검측IL-10적혈장농도.결과 흡연화음주증가료리환성대백반적풍험(OR위4.73화5.70,95％ CI위2.4 ～9.1;2.9～11.2,P＜0.01).성대백반환자재IL-10적-592화-819위점비대조조유경고적AC돌변빈솔(OR=1.93,95％ CI위0.97～ 3.81,P=0.05),재-1082위점유경고적AG돌변빈솔(OR =2.14,95％ CI위0.87 ～5.27,P=0.092);성대백반환자적혈장IL-10질량농도위(21.6 ±0.5)pg/ml((x)±s),대조조위(19.0±1.1) pg/ml,량자비교차이유통계학의의(t=2.08,P=0.043).기인형중함유G기인적단배체환자적혈장IL-10질량농도(24.3±5.7)pg/ml,비정상기인형단배체환자IL-10적농도고(19.9 ±4.7) pg/ml,량자비교차이유통계학의의(t=-2.64,P=0.008).결론 IL-10계동자구-1082/-819/-592다태성급혈장중고농도적IL-10여성대백반적발생유관,동시,돌변기인형여IL-10혈장농도유관.
Objective To investigated the association between interleukin-10(IL-10)-1082/-819/-592 promoter polymorphism,plasma IL-10 levels and risk of vocal leukoplakia.Methods A case-control study of 61 patients with vocal cords leukoplakia and 119 healthy subjects were performed.Genotypes for the IL-10 gene (IL-10-1082 G/A,-819 C/T and-592 C/A) were determined using pyrosequencing and plasma IL-10 levels were analyzed by ELISA.Results Smoking and alcohol consumption increased the risk of vocal leukoplakia (OR =4.73,95％ CI:2.4-9.1 ; OR =5.70,95％ CI:2.9-11.2,P ＜ 0.01).Patients with vocal cord leukoplakia had significantly higher frequencies of AC at-592 and-819 (OR =1.93,95％ CI:0.97-3.81,P=0.05) and AG at-1082(OR =2.14,95％ CI:0.87-5.27,P =0.092) than control.Vocal leukoplakiapatients had higher concentration of plasma IL-10 (21.6 ± 0.5)pg/ml ((x) ± s) than controls (19.0 ± 1.1) pg/ml(t =2.08,P =0.043) and the haplotype containing the G allele was associated with higher plasma IL-10 concentrations (24.3 ± 5.7) pg/ml compared with the ATA haplotype (19.9 ± 4.7)pg/ml (t =-2.64,P =0.008).Conclusions IL-10-1082/-819/-592 promoter polymorphism and high concentration of plasma IL-10 are associated with vocal cord leukoplakia,and the concentration of plasma IL-10 is associated with the genotypes of IL-10 promoter polymorphism.
