中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2013年
12期
1028-1031
,共4页
王维%周爱娥%黄益飞%项云%何於娟
王維%週愛娥%黃益飛%項雲%何於娟
왕유%주애아%황익비%항운%하어연
链球菌,肺炎%肺炎球菌感染%中耳炎%小鼠,近交C57BL
鏈毬菌,肺炎%肺炎毬菌感染%中耳炎%小鼠,近交C57BL
련구균,폐염%폐염구균감염%중이염%소서,근교C57BL
Streptococcus pneumoniae%Pneumococcal infections%Otitis media%Mice,inbred C57BL
目的 比较两种不同血清型肺炎链球菌致C57BL/6小鼠急性中耳炎的敏感性.方法 实验组C57BL/6小鼠经鼓膜穿刺分别接种血清型为TIGR4和6B的肺炎链球菌,对照组采用相同方法接种等体积磷酸盐缓冲液(PBS).观察小鼠发病情况,分别于感染后第1、3、5、7天制备中耳组织切片,HE染色观察中耳组织损伤及炎性细胞浸润变化,同时收集中耳灌洗液,检测灌洗液中细菌载量、炎性细胞数量和细胞因子含量.结果 6B组小鼠生存率明显低于TIGR4组和PBS对照组,差异具有统计学意义(P<0.01).接种肺炎链球菌后,小鼠中耳灌洗液中以中性粒细胞为主,TIGR4组募集的炎性细胞数量明显多于6B组;中耳灌洗液中白细胞介素6和肿瘤坏死因子α含量明显升高,其中TIGR4组细胞因子水平均明显高于6B组;6B组小鼠中耳内的细菌清除更慢.结论 在C57 BL/6小鼠急性中耳炎模型中,6B型肺炎链球菌比TIGR4型致病性更强.
目的 比較兩種不同血清型肺炎鏈毬菌緻C57BL/6小鼠急性中耳炎的敏感性.方法 實驗組C57BL/6小鼠經鼓膜穿刺分彆接種血清型為TIGR4和6B的肺炎鏈毬菌,對照組採用相同方法接種等體積燐痠鹽緩遲液(PBS).觀察小鼠髮病情況,分彆于感染後第1、3、5、7天製備中耳組織切片,HE染色觀察中耳組織損傷及炎性細胞浸潤變化,同時收集中耳灌洗液,檢測灌洗液中細菌載量、炎性細胞數量和細胞因子含量.結果 6B組小鼠生存率明顯低于TIGR4組和PBS對照組,差異具有統計學意義(P<0.01).接種肺炎鏈毬菌後,小鼠中耳灌洗液中以中性粒細胞為主,TIGR4組募集的炎性細胞數量明顯多于6B組;中耳灌洗液中白細胞介素6和腫瘤壞死因子α含量明顯升高,其中TIGR4組細胞因子水平均明顯高于6B組;6B組小鼠中耳內的細菌清除更慢.結論 在C57 BL/6小鼠急性中耳炎模型中,6B型肺炎鏈毬菌比TIGR4型緻病性更彊.
목적 비교량충불동혈청형폐염련구균치C57BL/6소서급성중이염적민감성.방법 실험조C57BL/6소서경고막천자분별접충혈청형위TIGR4화6B적폐염련구균,대조조채용상동방법접충등체적린산염완충액(PBS).관찰소서발병정황,분별우감염후제1、3、5、7천제비중이조직절편,HE염색관찰중이조직손상급염성세포침윤변화,동시수집중이관세액,검측관세액중세균재량、염성세포수량화세포인자함량.결과 6B조소서생존솔명현저우TIGR4조화PBS대조조,차이구유통계학의의(P<0.01).접충폐염련구균후,소서중이관세액중이중성립세포위주,TIGR4조모집적염성세포수량명현다우6B조;중이관세액중백세포개소6화종류배사인자α함량명현승고,기중TIGR4조세포인자수평균명현고우6B조;6B조소서중이내적세균청제경만.결론 재C57 BL/6소서급성중이염모형중,6B형폐염련구균비TIGR4형치병성경강.
Objective To compare the sensitivities of two different serotypes of Streptococcus pneumoniae in acute otitis media of C57BL/6 mice.Methods The middle ear cavity of C57BL/6 mice were inoculated via intratympanic injection of Streptococcus pneumoniae TIGR4 or 6B 1 × 108 CFU,and the control group were inoculated equivalent phosphate buffered solution (PBS).The incidence of mice,development of otitis media and the middle ear lavage fluid pathological changes by HE staining,as well as cell counts and cytokine levels were investigated.Results After inoculated with Streptococcus pneumoniae TIGR4,6B and PBS,the survival rate of 6B group was significantly less than TIGR4 group and PBS control group (P <0.01).Inflammatory cells in the middle ear cavity were mainly neutrophils,and the inflammatory cells recruitment in TIGR4 group were more than 6B group; The levels of IL-6 and TNF-α in the middle ear lavage fluid in T1GR4 group and 6B group were significantly increased compared with PBS control group,while the TIGR4 group were significantly increased compared with 6B group ;6B group had delayed bacterial clearance in the middle ear.Conclusion The study implied that Streptococcus pneumoniae 6B had higher pathogenicity for acute otitis media in C57BL/6 mice than TIGR4.