CAJ | 학술논문

目的利用基因表达谱芯片,寻找有意义的差异表达基因,从而探讨喉癌发生的分子生物学机制.方法采用Illumina人类全基因组表达谱芯片检测10对喉癌及癌旁组织的基因表达情况.提取总RNA,反转录成cDNA,将标记的cDNA与基因表达谱芯片杂交,最后读取数据及获取图像,所有样品均通过质控检测.结果采用Illumina Genomstudio1.9.0软件数据处理(P＜0.05或差异分值＞ 13,差异分值＜-13),多重置换t检验校正(假阳性率＜0.05),喉癌组织及癌旁组织的比较分析发现,在喉癌组织中差异表达的基因共有426个(上调222个,下调204个),主要参与调节细胞周期和增殖、染色体分离、细胞的有丝分裂和减数分裂等,同时也参与了多条与肿瘤发生相关的信号通路,例如:代谢通路、细胞周期、谷胱甘肽代谢、黏蛋白O多糖生物合成、药物代谢细胞色素P450等.结论通过癌组织和癌旁组织的差异表达研究,筛选出相关基因,并对其功能及定位信息进一步分析,从多基因相互作用的角度探究喉鳞状细胞癌的发生发展机制.
목적 이용기인표체보심편,심조유의의적차이표체기인,종이탐토후암발생적분자생물학궤제.방법 채용Illumina인류전기인조표체보심편검측10대후암급암방조직적기인표체정황.제취총RNA,반전록성cDNA,장표기적cDNA여기인표체보심편잡교,최후독취수거급획취도상,소유양품균통과질공검측.결과 채용Illumina Genomstudio1.9.0연건수거처리(P＜0.05혹차이분치＞ 13,차이분치＜-13),다중치환t검험교정(가양성솔＜0.05),후암조직급암방조직적비교분석발현,재후암조직중차이표체적기인공유426개(상조222개,하조204개),주요삼여조절세포주기화증식、염색체분리、세포적유사분렬화감수분렬등,동시야삼여료다조여종류발생상관적신호통로,례여:대사통로、세포주기、곡광감태대사、점단백O다당생물합성、약물대사세포색소P450등.결론 통과암조직화암방조직적차이표체연구,사선출상관기인,병대기공능급정위신식진일보분석,종다기인상호작용적각도탐구후린상세포암적발생발전궤제.
Objective To identify the genes differentially expressed and underlying molecular mechanism in laryngeal squamous cell carcinoma by using cDNA microarrays.Methods Using Illumina Human HT-12 BeadChip,gene expressions were detected in ten pairs of laryngeal cancer tissues and adjacent normal tissues.Total RNA was extracted and reverse-transcribed into cDNA.Labeled cDNA were hybridized with cDNA microarray,data were read and images were scanned.All the samples had passed quality control testing.Results Through Illumina Gcnomstudio 1.9.0 Data processing software (P ＜ 0.05 or diffscore ＞ 13,diffscore ＜-13) and multiple displacement t test (FDR ＜ 0.05),426 genes showed statistically significant differences in expressions between laryngeal tumor tissues and corresponding adjacent normal tissues,with 222 up-regulated genes and 204 down-regulated genes in laryngeal cancer tissues.These up-or down-regulated genes were indicated to involve in cellular processes relevant to the cancer phenotype,such as proliferation,cell cycle,chromosome segregation,mitosis and meiosis.These differentialy expressed genes also took part in cancer related signaling pathways as well,for instance,metabolic pathways,cell cycle,DNA replication,glutathione metabolism,mucin type O-Glycan biosynthesis,drug metabolism-cytochrome P450 and so on.Conclusion The set of genes identified here and their functional annotations contribute to a better understanding of the pathogenesis of laryngeal squamous cell carcinoma from the view of multiple gene interactions and provide candidate markers for improving diagnosis,prognosis and treatment of this cancer.