CAJ | 학술논문

目的比较不同波形和参数的脉冲电刺激诱发周围性面神经麻痹新西兰白兔眨眼运动的效果,同时建立一个功能性电刺激系统,用以恢复单侧周围性面神经麻痹兔的双侧同步眨眼功能.方法 24只新西兰白兔,完全随机法分为Ⅰ、Ⅱ、Ⅲ组,每组8只.分别切断右侧面神经总干,制作单侧周围性面神经麻痹模型;采用电脉冲串分别刺激其双侧眼轮匝肌,诱发眨眼运动;Ⅰ、Ⅱ、Ⅲ组兔的刺激波形分别为方波、正弦波和三角波,每只动物分别接受三种载波频率(25、50和100 Hz)、九种波宽(1～9 ms)的脉冲刺激;利用SPSS 16.0统计软件比较不同波形和刺激参数之间诱发眨眼反应所需电压阈值和总电荷量的差别.然后任意选取6只单侧周围性面神经麻痹兔,用生理记录仪和电刺激器组成的功能性电刺激系统来记录其健侧眨眼时眼轮匝肌的肌电信号,并以此为触发信号,触发麻痹侧眼轮匝肌的电刺激,观察其眨眼动作.结果 25 Hz的电脉冲串在诱发眨眼的过程中会发生眼睑的持续颤动,不能产生平滑的眨眼运动;而50 Hz和100 Hz载波频率的脉冲串刺激可以诱发接近自然状态的眨眼反应.方波诱发完全眨眼所需的阈电压远小于其他两种波形(P＜0.05).三种波形刺激诱发眨眼反应所需的总电荷量差异较小.在刺激频率相同时,三种波形诱发完全眨眼所需的总电荷量随着脉冲波宽的增加几乎呈线性增加.100 Hz的脉冲串刺激诱发眨眼所需的电压小于50 Hz(P ＜0.05),但是其消耗的电荷量远高于50 Hz的脉冲串(P＜0.05).6只兔在健侧产生诱发性眨眼时,功能性电刺激系统均能够成功恢复麻痹侧的同步眨眼运动.结论 50 Hz的方波电脉冲最适合用于刺激兔眼轮匝肌诱发眨眼,用健侧眼轮匝肌的肌电信号作为患侧眼轮匝肌电刺激的触发信号,可以恢复单侧周围性面神经麻痹兔的双侧同步眨眼. 目的比較不同波形和參數的脈遲電刺激誘髮週圍性麵神經痳痺新西蘭白兔眨眼運動的效果,同時建立一箇功能性電刺激繫統,用以恢複單側週圍性麵神經痳痺兔的雙側同步眨眼功能.方法 24隻新西蘭白兔,完全隨機法分為Ⅰ、Ⅱ、Ⅲ組,每組8隻.分彆切斷右側麵神經總榦,製作單側週圍性麵神經痳痺模型;採用電脈遲串分彆刺激其雙側眼輪匝肌,誘髮眨眼運動;Ⅰ、Ⅱ、Ⅲ組兔的刺激波形分彆為方波、正絃波和三角波,每隻動物分彆接受三種載波頻率(25、50和100 Hz)、九種波寬(1～9 ms)的脈遲刺激;利用SPSS 16.0統計軟件比較不同波形和刺激參數之間誘髮眨眼反應所需電壓閾值和總電荷量的差彆.然後任意選取6隻單側週圍性麵神經痳痺兔,用生理記錄儀和電刺激器組成的功能性電刺激繫統來記錄其健側眨眼時眼輪匝肌的肌電信號,併以此為觸髮信號,觸髮痳痺側眼輪匝肌的電刺激,觀察其眨眼動作.結果 25 Hz的電脈遲串在誘髮眨眼的過程中會髮生眼瞼的持續顫動,不能產生平滑的眨眼運動;而50 Hz和100 Hz載波頻率的脈遲串刺激可以誘髮接近自然狀態的眨眼反應.方波誘髮完全眨眼所需的閾電壓遠小于其他兩種波形(P＜0.05).三種波形刺激誘髮眨眼反應所需的總電荷量差異較小.在刺激頻率相同時,三種波形誘髮完全眨眼所需的總電荷量隨著脈遲波寬的增加幾乎呈線性增加.100 Hz的脈遲串刺激誘髮眨眼所需的電壓小于50 Hz(P ＜0.05),但是其消耗的電荷量遠高于50 Hz的脈遲串(P＜0.05).6隻兔在健側產生誘髮性眨眼時,功能性電刺激繫統均能夠成功恢複痳痺側的同步眨眼運動.結論 50 Hz的方波電脈遲最適閤用于刺激兔眼輪匝肌誘髮眨眼,用健側眼輪匝肌的肌電信號作為患側眼輪匝肌電刺激的觸髮信號,可以恢複單側週圍性麵神經痳痺兔的雙側同步眨眼.
목적 비교불동파형화삼수적맥충전자격유발주위성면신경마비신서란백토잡안운동적효과,동시건립일개공능성전자격계통,용이회복단측주위성면신경마비토적쌍측동보잡안공능.방법 24지신서란백토,완전수궤법분위Ⅰ、Ⅱ、Ⅲ조,매조8지.분별절단우측면신경총간,제작단측주위성면신경마비모형;채용전맥충천분별자격기쌍측안륜잡기,유발잡안운동;Ⅰ、Ⅱ、Ⅲ조토적자격파형분별위방파、정현파화삼각파,매지동물분별접수삼충재파빈솔(25、50화100 Hz)、구충파관(1～9 ms)적맥충자격;이용SPSS 16.0통계연건비교불동파형화자격삼수지간유발잡안반응소수전압역치화총전하량적차별.연후임의선취6지단측주위성면신경마비토,용생리기록의화전자격기조성적공능성전자격계통래기록기건측잡안시안륜잡기적기전신호,병이차위촉발신호,촉발마비측안륜잡기적전자격,관찰기잡안동작.결과 25 Hz적전맥충천재유발잡안적과정중회발생안검적지속전동,불능산생평활적잡안운동;이50 Hz화100 Hz재파빈솔적맥충천자격가이유발접근자연상태적잡안반응.방파유발완전잡안소수적역전압원소우기타량충파형(P＜0.05).삼충파형자격유발잡안반응소수적총전하량차이교소.재자격빈솔상동시,삼충파형유발완전잡안소수적총전하량수착맥충파관적증가궤호정선성증가.100 Hz적맥충천자격유발잡안소수적전압소우50 Hz(P ＜0.05),단시기소모적전하량원고우50 Hz적맥충천(P＜0.05).6지토재건측산생유발성잡안시,공능성전자격계통균능구성공회복마비측적동보잡안운동.결론 50 Hz적방파전맥충최괄합용우자격토안륜잡기유발잡안,용건측안륜잡기적기전신호작위환측안륜잡기전자격적촉발신호,가이회복단측주위성면신경마비토적쌍측동보잡안.
Objective Tocompare the effects of different waveforms and parameters of electrical stimulation to elicit a blink,and construct a functional electrical stimulation (FES) system to restore synchronous blink in unilateral facial nerve palsy (FNP).Methods Firstly,twenty-four rabbits were surgically induced unilateral FNP and were divided into three groups,who received square,sine and triangle pulse wareforms,respectirely.Both the healthy and the paralysis eyelids of the rabbits received pulse train stimulation to produce a blink in both eyes.For each rabbit,twenty-seven combinations of frequencies (25 Hz,50 Hz and 100 Hz) and nine pulse widths (1-9 ms) were stimulated.The threshold amplitude and electric charge to elicit a blink was compared between different waveforms and different parameters.Secondly,a FES system was constructed to treat six surgically induced unilateral FNP rabbit chosen in the twenty-four rabbits,it consisted by an electromyogram (EMG) amplifier module which record the EMG of the healthy muscle,and a stimulator which received the EMG input and output a pulse train stimulation when triggered by the EMG.Results When the carrier frequency of the pulse train was 25 Hz,it was not able to induce a smooth blink.However,when the carrier frequencies were 50 Hz and 100 Hz,a smooth blink could be induced.The voltage required by 100 Hz was lower than 50 Hz,but it cost more electric charge.The amplitude that square waveforms required was far lower than sine and triangle,but the electric charge between the three waveforms was similar.Synchronous blink could be restored in the six unilateral FNP rabbits with the FES system.Conclusions To elicit a blink,square pulse train delivered in 50 Hz is a preferable option.The motion of the healthy eyelids as a source of information for stimulation of the paralyzed sides can restore the synchronous blink in unilateral FNP rabbits.