中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2014年
8期
668-674
,共7页
赵延明%张媛%崔晓辉%阮宇%张罗
趙延明%張媛%崔曉輝%阮宇%張囉
조연명%장원%최효휘%원우%장라
鼻窦炎%多态性,单核苷酸%酰基羧酸水解酶类
鼻竇炎%多態性,單覈苷痠%酰基羧痠水解酶類
비두염%다태성,단핵감산%선기최산수해매류
Sinusitis%Polymorphism,single nucleotide%Carboxylic ester hydrolases
目的 进行慢性鼻-鼻窦炎(chronic rhinosinusitis,CRS)风险基因在中国汉族CRS人群中的验证性研究.方法 选取2008年2月至2009年7月首都医科大学附属北京同仁医院CRS伴鼻息肉患者306例,CRS不伴鼻息肉患者332例及健康对照组315例作为研究对象.选择加拿大学者研究发现的与CRS显著相关的10个基因位点进行单核苷酸多态性(single nucleotide polymorphism,SNP)分型验证.等位基因频率和基因型频率采用频率计数法计算,应用x2检验或确切概率法进行分析,最后得出的统计学结果进行Bonferroni多重矫正.以SPSS 13.0软件进行统计学分析.结果 酰基羧酸水解酶(acyloxyacyl hydroxylase,AOAH)基因的SNP位点rs4504543与CRS具有相关性(OR=0.559 0,P=1.95 ×10-5).在CRS亚型的分析中发现,rs4504543位点与CRS不伴鼻息肉具有相关性(OR=0.284 8,P=3.47 ×10-12).结论 AOAH基因多态性在中国汉族人群中与CRS发病具有相关性,AOAH多态性改变可降低中国汉族人群CRS的发病风险.
目的 進行慢性鼻-鼻竇炎(chronic rhinosinusitis,CRS)風險基因在中國漢族CRS人群中的驗證性研究.方法 選取2008年2月至2009年7月首都醫科大學附屬北京同仁醫院CRS伴鼻息肉患者306例,CRS不伴鼻息肉患者332例及健康對照組315例作為研究對象.選擇加拿大學者研究髮現的與CRS顯著相關的10箇基因位點進行單覈苷痠多態性(single nucleotide polymorphism,SNP)分型驗證.等位基因頻率和基因型頻率採用頻率計數法計算,應用x2檢驗或確切概率法進行分析,最後得齣的統計學結果進行Bonferroni多重矯正.以SPSS 13.0軟件進行統計學分析.結果 酰基羧痠水解酶(acyloxyacyl hydroxylase,AOAH)基因的SNP位點rs4504543與CRS具有相關性(OR=0.559 0,P=1.95 ×10-5).在CRS亞型的分析中髮現,rs4504543位點與CRS不伴鼻息肉具有相關性(OR=0.284 8,P=3.47 ×10-12).結論 AOAH基因多態性在中國漢族人群中與CRS髮病具有相關性,AOAH多態性改變可降低中國漢族人群CRS的髮病風險.
목적 진행만성비-비두염(chronic rhinosinusitis,CRS)풍험기인재중국한족CRS인군중적험증성연구.방법 선취2008년2월지2009년7월수도의과대학부속북경동인의원CRS반비식육환자306례,CRS불반비식육환자332례급건강대조조315례작위연구대상.선택가나대학자연구발현적여CRS현저상관적10개기인위점진행단핵감산다태성(single nucleotide polymorphism,SNP)분형험증.등위기인빈솔화기인형빈솔채용빈솔계수법계산,응용x2검험혹학절개솔법진행분석,최후득출적통계학결과진행Bonferroni다중교정.이SPSS 13.0연건진행통계학분석.결과 선기최산수해매(acyloxyacyl hydroxylase,AOAH)기인적SNP위점rs4504543여CRS구유상관성(OR=0.559 0,P=1.95 ×10-5).재CRS아형적분석중발현,rs4504543위점여CRS불반비식육구유상관성(OR=0.284 8,P=3.47 ×10-12).결론 AOAH기인다태성재중국한족인군중여CRS발병구유상관성,AOAH다태성개변가강저중국한족인군CRS적발병풍험.
Objective To replicate the polymorphisms in risk genes of chronic rhinosinusitis in a Chinese Han population.Methods Enrolled in this study were CRS patients with nasal polyps (n =306,CRSwNP),CRS patients without nasal polyps (n =332,CRSsNP),and controls (n =315) in a Chinese population.All the patients were recruited from clinic of the department of Otorhinolaryngology of Beijing Tongren Hospital between 2008 February and 2009 July.A total of 10 single nucleotide polymorphisms (SNPs) selected from previous identified SNPs associated with CRS in Canadian population were individually genotyped.Allele and genotype frequencies were calculated by frequency counting,the chi-square test or exact method were applied to analyze the results.Final results were corrected by Bonferroni multiple correction.SPSS 13.0 software was used for statistical analysis.Results One SNP in AOAH gene (rs4504543,P =1.95 × 10-5,OR =0.559 0) was identified to be significantly associated with whole CRS cohort.After subgroup analysis for the presence of nasal polyps (CRSwNP and CRSsNP),the same SNP in AOAH (rs4504543,P =3.47 × 10-12,OR =0.284 8) was also found to be significantly associated with CRSsNP cohorts.Conclusions AOAH was significantly associated with CRS and its polymorphisms might play a role in the susceptibility to develop CRS in Chinese population.
