CAJ | 학술논문

目的通过建立声诱发短潜伏期负电位(acoustically evoked short latency negative response,ASNR)豚鼠模型,直流电损毁前庭神经核或蜗神经核,验证ASNR的神经来源.方法 24只健康豚鼠采用随机数字表法分为两组,正常对照组8只(16耳),致聋组16只(32耳).致聋组应用卡那霉素和利尿酸联合致聋,根据ASNR引出与否又分为ASNR组和非ASNR组.对照组、ASNR组及非ASNR组按损毁核团不同又分为前庭神经核损毁组及蜗神经核损毁组.所有动物均分别进行前庭神经核及蜗神经核定位,直流电损毁神经核团,24 h后观察声诱发脑干电位的改变.处死动物后脑干标本经冰冻切片及染色,显微镜下验证损毁部位.结果致聋组16只豚鼠(32耳)中10耳出现ASNR(31.3％).正常对照组豚鼠前庭神经核损毁后听性脑干反应(ABR)波形无明显改变,蜗神经核损毁后ABR仅余Ⅰ波.ASNR组中前庭神经核损毁组(7耳)ASNR均消失,而蜗神经核损毁组(3耳)ASNR均未消失;非ASNR组前庭神经核或蜗神经核损毁后ABR波形均无明显改变.脑干切片证实直流电破坏部位准确.结论 ASNR的责任神经核是前庭神经核,与蜗神经核无关.
목적 통과건립성유발단잠복기부전위(acoustically evoked short latency negative response,ASNR)돈서모형,직류전손훼전정신경핵혹와신경핵,험증ASNR적신경래원.방법 24지건강돈서채용수궤수자표법분위량조,정상대조조8지(16이),치롱조16지(32이).치롱조응용잡나매소화이뇨산연합치롱,근거ASNR인출여부우분위ASNR조화비ASNR조.대조조、ASNR조급비ASNR조안손훼핵단불동우분위전정신경핵손훼조급와신경핵손훼조.소유동물균분별진행전정신경핵급와신경핵정위,직류전손훼신경핵단,24 h후관찰성유발뇌간전위적개변.처사동물후뇌간표본경빙동절편급염색,현미경하험증손훼부위.결과 치롱조16지돈서(32이)중10이출현ASNR(31.3％).정상대조조돈서전정신경핵손훼후은성뇌간반응(ABR)파형무명현개변,와신경핵손훼후ABR부여Ⅰ파.ASNR조중전정신경핵손훼조(7이)ASNR균소실,이와신경핵손훼조(3이)ASNR균미소실;비ASNR조전정신경핵혹와신경핵손훼후ABR파형균무명현개변.뇌간절편증실직류전파배부위준학.결론 ASNR적책임신경핵시전정신경핵,여와신경핵무관.
Objective This study established a model of acoustically evoked short latency negative response (ASNR) in guinea pigs.Stereotaxic coordinate guided electrolytic lesion was applied to animal brainstem nuclei,the vestibular nucleus and the cochlear nucleus,to define the neural origin of ASNR.Methods Twenty four guinea pigs with normal hearing were randomly divided into the control group (8 subjects,16 ears) and the deafened group (16 subjects,32 ears).Each animal experienced the auditory brainstem response (ABR) test.According to the presence of ASNR,the deafened group was further divided into ASNR group and non-ASNR group.Electrolytic lesion was conducted to the vestibular nucleus and cochlear nucleus respectively,followed by ABR test.The lesion structures were verified by brainstem slice and microscope.Results In deafened group,the ASNR was elicited in 10 ears (31.3％).The ASNR was eliminated due to the electrolytic destruction to the vestibular nucleus,but it remained unchanged after the same procedure to the cochlear nucleus.Conclusion It is clear that the ASNR is originated from the vestibular nucleus,but not the cochlear nucleus.