中华儿科杂志
中華兒科雜誌
중화인과잡지
Chinese Journal of Pediatrics
2013年
3期
172-177
,共6页
吴斌%赖乾坤%刘俊红%林春
吳斌%賴乾坤%劉俊紅%林春
오빈%뢰건곤%류준홍%림춘
痛觉过敏%食物过敏%肥大细胞%P物质%腹痛相关性功能性胃肠病
痛覺過敏%食物過敏%肥大細胞%P物質%腹痛相關性功能性胃腸病
통각과민%식물과민%비대세포%P물질%복통상관성공능성위장병
Hyperalgesia%Food hypersensitivity%Mast cells%Substance P%Functional gastrointestinal disorders
目的 探讨食物过敏(FA)对幼鼠内脏痛觉敏感性影响及结肠黏膜P物质(SP)异常表达在FA幼鼠内脏痛觉高敏形成中作用.方法 3周龄雌性SD大鼠20只,随机分FA组和对照组(NS组),各10只.FA组通过卵白蛋白(OVA)低剂量腹腔注射基础致敏及高剂量灌胃激发建立幼鼠OVA-FA模型；检测血清OVA-IgE含量、观察肠黏膜病理改变及嗜酸性粒细胞、肥大细胞浸润情况进行幼鼠FA模型评价.通过观察两组幼鼠在不同压力结直肠扩张(CRD)刺激后的腹外斜肌(EOMA)放电测量进行内脏痛觉敏感性评价.应用免疫组织化学染色及计算机图像分析系统对结肠SP进行积分吸光度值半定量分析.采用SPSS13.0软件包进行统计分析,α=0.05为显著性检验标准.结果 与对照组比较,FA组幼鼠血清OVA-IgE含量、空肠黏膜嗜酸性粒细胞和肥大细胞数及肥大细胞脱颗粒百分率均明显升高,差异有统计学意义(P均＜0.01).在CRD为0、15、30、45、60、75 mm Hg(1 mm Hg=0.133 kPa)时,FA组腹外斜肌放电幅值分别为(17.74 ±0.72)、(18.63±1.72)、(22.55±1.70)、(28.63 ±7.00)、(33.97 ±7.34)、(37.26±8.40) μV,NS组分别为(17.43±1.18)、(17.27±1.16)、(17.73±1.42)、(19.55±3.54)、(23.29 ±5.46)、(25.20±4.75) μV.随CRD增加,腹外斜肌放电幅值较前明显升高,CRD 30、45、60、75 mm Hg时,两组差异有统计学意义(P均＜0.01).FA组、NS组幼鼠结肠SP积分吸光度值分别为(247.12 ±90.83)、(103.90 ±58.94),差异有统计学意义(t=4.183,P＜0.01).结论 OVA低剂量腹腔注射基础致敏及高剂量灌胃激发可建立幼鼠FA模型.生命早期FA可导致幼鼠腹外斜肌放电幅值明显升高并伴有结肠SP表达增加,出现慢性内脏痛觉高敏感.生命早期FA导致的内脏痛觉高敏感性发生可能与肠黏膜肥大细胞表达增多、脱颗粒及肠道SP的异常表达有关.
目的 探討食物過敏(FA)對幼鼠內髒痛覺敏感性影響及結腸黏膜P物質(SP)異常錶達在FA幼鼠內髒痛覺高敏形成中作用.方法 3週齡雌性SD大鼠20隻,隨機分FA組和對照組(NS組),各10隻.FA組通過卵白蛋白(OVA)低劑量腹腔註射基礎緻敏及高劑量灌胃激髮建立幼鼠OVA-FA模型；檢測血清OVA-IgE含量、觀察腸黏膜病理改變及嗜痠性粒細胞、肥大細胞浸潤情況進行幼鼠FA模型評價.通過觀察兩組幼鼠在不同壓力結直腸擴張(CRD)刺激後的腹外斜肌(EOMA)放電測量進行內髒痛覺敏感性評價.應用免疫組織化學染色及計算機圖像分析繫統對結腸SP進行積分吸光度值半定量分析.採用SPSS13.0軟件包進行統計分析,α=0.05為顯著性檢驗標準.結果 與對照組比較,FA組幼鼠血清OVA-IgE含量、空腸黏膜嗜痠性粒細胞和肥大細胞數及肥大細胞脫顆粒百分率均明顯升高,差異有統計學意義(P均＜0.01).在CRD為0、15、30、45、60、75 mm Hg(1 mm Hg=0.133 kPa)時,FA組腹外斜肌放電幅值分彆為(17.74 ±0.72)、(18.63±1.72)、(22.55±1.70)、(28.63 ±7.00)、(33.97 ±7.34)、(37.26±8.40) μV,NS組分彆為(17.43±1.18)、(17.27±1.16)、(17.73±1.42)、(19.55±3.54)、(23.29 ±5.46)、(25.20±4.75) μV.隨CRD增加,腹外斜肌放電幅值較前明顯升高,CRD 30、45、60、75 mm Hg時,兩組差異有統計學意義(P均＜0.01).FA組、NS組幼鼠結腸SP積分吸光度值分彆為(247.12 ±90.83)、(103.90 ±58.94),差異有統計學意義(t=4.183,P＜0.01).結論 OVA低劑量腹腔註射基礎緻敏及高劑量灌胃激髮可建立幼鼠FA模型.生命早期FA可導緻幼鼠腹外斜肌放電幅值明顯升高併伴有結腸SP錶達增加,齣現慢性內髒痛覺高敏感.生命早期FA導緻的內髒痛覺高敏感性髮生可能與腸黏膜肥大細胞錶達增多、脫顆粒及腸道SP的異常錶達有關.
목적 탐토식물과민(FA)대유서내장통각민감성영향급결장점막P물질(SP)이상표체재FA유서내장통각고민형성중작용.방법 3주령자성SD대서20지,수궤분FA조화대조조(NS조),각10지.FA조통과란백단백(OVA)저제량복강주사기출치민급고제량관위격발건립유서OVA-FA모형；검측혈청OVA-IgE함량、관찰장점막병리개변급기산성립세포、비대세포침윤정황진행유서FA모형평개.통과관찰량조유서재불동압력결직장확장(CRD)자격후적복외사기(EOMA)방전측량진행내장통각민감성평개.응용면역조직화학염색급계산궤도상분석계통대결장SP진행적분흡광도치반정량분석.채용SPSS13.0연건포진행통계분석,α=0.05위현저성검험표준.결과 여대조조비교,FA조유서혈청OVA-IgE함량、공장점막기산성립세포화비대세포수급비대세포탈과립백분솔균명현승고,차이유통계학의의(P균＜0.01).재CRD위0、15、30、45、60、75 mm Hg(1 mm Hg=0.133 kPa)시,FA조복외사기방전폭치분별위(17.74 ±0.72)、(18.63±1.72)、(22.55±1.70)、(28.63 ±7.00)、(33.97 ±7.34)、(37.26±8.40) μV,NS조분별위(17.43±1.18)、(17.27±1.16)、(17.73±1.42)、(19.55±3.54)、(23.29 ±5.46)、(25.20±4.75) μV.수CRD증가,복외사기방전폭치교전명현승고,CRD 30、45、60、75 mm Hg시,량조차이유통계학의의(P균＜0.01).FA조、NS조유서결장SP적분흡광도치분별위(247.12 ±90.83)、(103.90 ±58.94),차이유통계학의의(t=4.183,P＜0.01).결론 OVA저제량복강주사기출치민급고제량관위격발가건립유서FA모형.생명조기FA가도치유서복외사기방전폭치명현승고병반유결장SP표체증가,출현만성내장통각고민감.생명조기FA도치적내장통각고민감성발생가능여장점막비대세포표체증다、탈과립급장도SP적이상표체유관.
Objective To explore the effect of food allergy (FA) on the development of visceralgia sensibility and the substance P (SP) expression in colon of developing rats with FA.Method Three-week old female Sprague-Dawley (SD) rats were randomly divided into two groups (n =10 in each).The rats in FA group were sensitized with ovalbumin (OVA) 40 μg and Al(OH)3 1 mg suspension solution (0.2 ml)intraperitoneal (i.p.) injection on day 0,only OVA 40 μg solution i.p.on day 2,4,7,9,11,respectively,and the rats were challenged by gavage with OVA solution 30 mg (2 ml) on day 20,24,28,30.The rats in non-sensitized (NS) group were not challenged except handled in the same ways.The serum OVA-IgE were determined by enzyme-linked immuno sorbent assay (ELISA) on day 0,30.Jejunum segments were used to observe morphological structure,the expression of eosinophils,and the density and the percentage of degranulation of mast cells (MC).The rats were appraised for the pain sensibility of intestinal tract under colorectal distension irritation by the electrophysiological method on external oblique in the 18-24 hr after the last challenge.Colons were used to analyze the expression of SP through immunohistochemical staining and computer image analyzing system.Result The serum OVA-IgE concentration and the eosinophils,mast cell,the percentage of mast cells degranulation in FA group were more than NS group (P ＜ 0.01).The amplitudes of spike external oblique muscle of abdomen (EOMA,μV) of the FA group under the colorectal distension (CRD) pressures at 0,15,30,45,60,75 mm Hg were (17.74 ±0.72),(18.63 ± 1.72),(22.55 ± 1.70),(28.63 ±7.00),(33.97 ±7.34),(37.26 ±8.40),and (17.43±1.18),(17.27±1.16),(17.73±1.42),(19.55 ±3.54),(23.29 ±5.46),(25.20 ±4.75) in NS group.With the CRD pressure increased,the amplitudes of spike EOMA increased significantly.There were significant differences between groups under the CRD pressures at 30,45,60,75 mm Hg (F =47.470,13.367,13.317,15.390,P ＜0.01).The expressions of colons SP in FA group and NS group are 247.12 ± 90.83 and 103.90 ± 58.94,respectively (t =4.183,P ＜ 0.01).Conclusion Sensitization through i.p.pathway and challenge by gavage with OVA in early life could result in FA in young SD rats.FA in early life enabled the amplitudes of spike EOMA and the expression of colons SP increase significantly.It may be related to increase in amount and degranulation of MC and SP abnormal expression in colon,which could lead to the development of visceralgia sensibility.
