中华儿科杂志
中華兒科雜誌
중화인과잡지
Chinese Journal of Pediatrics
2014年
9期
667-672
,共6页
食物过敏%全反式维甲酸%治疗
食物過敏%全反式維甲痠%治療
식물과민%전반식유갑산%치료
Food allergy%All-trans retinoic acid%Therapy
目的 研究全反式维甲酸(all-trans retinoic acid,atRA)对卵清蛋白(ovalbumin,OVA)过敏小鼠的免疫治疗效果.方法 将40只OVA过敏小鼠按atRA干预剂量不同分为高剂量atRA组(100 mg/kg)、中剂量atRA组(50 mg/kg)、低剂量atRA组(20 mg/kg)以及对照组(无atRA).观察不同剂量atRA干预12 d后小鼠体重、腹泻及肠道形态学变化;并采用酶联免疫吸附试验检测血清OVA-IgE、粪便总IgA及OVA-IgA含量;流式细胞仪检测肠系膜淋巴结中CD4+ CD25+ FoxP3+T细胞占总CD4+T细胞百分比.结果 与对照组相比,低剂量atRA组小鼠血清OVA-IgE(1.221±0.367比0.793±0.616)和粪便OVA-IgA(1.573±0.656比0.905±0.279)显著降低(t=3.140,P=0.006;t＝2.827,P=0.012);小鼠空肠形态学基本正常;肠系膜淋巴结中CD4+ CD25+ FoxP3+T细胞占总CD4+T细胞百分比(10.641±1.218比10.936±0.954)差异无统计学意义(t=0.539,P=0.598).而中、高剂量atRA两组小鼠血清OVA-IgE(1.109 ±0.319;0.938±0.281)、粪便总IgA(1.104±0.358;1.069 ±0.221)及肠系膜淋巴结中CD4+ CD25+ FoxP3+T细胞占总CD4+T细胞百分比(9.968±2.465;12.146 ±2.159)均与对照组间差异无统计学意义(P＞0.05);同时中、高剂量atRA干预会造成小鼠体重下降及肠道黏膜修复异常.结论 低剂量atRA对OVA过敏小鼠可能有一定的免疫抑制作用;而中、高剂量atRA对OVA过敏小鼠无免疫治疗作用.
目的 研究全反式維甲痠(all-trans retinoic acid,atRA)對卵清蛋白(ovalbumin,OVA)過敏小鼠的免疫治療效果.方法 將40隻OVA過敏小鼠按atRA榦預劑量不同分為高劑量atRA組(100 mg/kg)、中劑量atRA組(50 mg/kg)、低劑量atRA組(20 mg/kg)以及對照組(無atRA).觀察不同劑量atRA榦預12 d後小鼠體重、腹瀉及腸道形態學變化;併採用酶聯免疫吸附試驗檢測血清OVA-IgE、糞便總IgA及OVA-IgA含量;流式細胞儀檢測腸繫膜淋巴結中CD4+ CD25+ FoxP3+T細胞佔總CD4+T細胞百分比.結果 與對照組相比,低劑量atRA組小鼠血清OVA-IgE(1.221±0.367比0.793±0.616)和糞便OVA-IgA(1.573±0.656比0.905±0.279)顯著降低(t=3.140,P=0.006;t＝2.827,P=0.012);小鼠空腸形態學基本正常;腸繫膜淋巴結中CD4+ CD25+ FoxP3+T細胞佔總CD4+T細胞百分比(10.641±1.218比10.936±0.954)差異無統計學意義(t=0.539,P=0.598).而中、高劑量atRA兩組小鼠血清OVA-IgE(1.109 ±0.319;0.938±0.281)、糞便總IgA(1.104±0.358;1.069 ±0.221)及腸繫膜淋巴結中CD4+ CD25+ FoxP3+T細胞佔總CD4+T細胞百分比(9.968±2.465;12.146 ±2.159)均與對照組間差異無統計學意義(P＞0.05);同時中、高劑量atRA榦預會造成小鼠體重下降及腸道黏膜脩複異常.結論 低劑量atRA對OVA過敏小鼠可能有一定的免疫抑製作用;而中、高劑量atRA對OVA過敏小鼠無免疫治療作用.
목적 연구전반식유갑산(all-trans retinoic acid,atRA)대란청단백(ovalbumin,OVA)과민소서적면역치료효과.방법 장40지OVA과민소서안atRA간예제량불동분위고제량atRA조(100 mg/kg)、중제량atRA조(50 mg/kg)、저제량atRA조(20 mg/kg)이급대조조(무atRA).관찰불동제량atRA간예12 d후소서체중、복사급장도형태학변화;병채용매련면역흡부시험검측혈청OVA-IgE、분편총IgA급OVA-IgA함량;류식세포의검측장계막림파결중CD4+ CD25+ FoxP3+T세포점총CD4+T세포백분비.결과 여대조조상비,저제량atRA조소서혈청OVA-IgE(1.221±0.367비0.793±0.616)화분편OVA-IgA(1.573±0.656비0.905±0.279)현저강저(t=3.140,P=0.006;t＝2.827,P=0.012);소서공장형태학기본정상;장계막림파결중CD4+ CD25+ FoxP3+T세포점총CD4+T세포백분비(10.641±1.218비10.936±0.954)차이무통계학의의(t=0.539,P=0.598).이중、고제량atRA량조소서혈청OVA-IgE(1.109 ±0.319;0.938±0.281)、분편총IgA(1.104±0.358;1.069 ±0.221)급장계막림파결중CD4+ CD25+ FoxP3+T세포점총CD4+T세포백분비(9.968±2.465;12.146 ±2.159)균여대조조간차이무통계학의의(P＞0.05);동시중、고제량atRA간예회조성소서체중하강급장도점막수복이상.결론 저제량atRA대OVA과민소서가능유일정적면역억제작용;이중、고제량atRA대OVA과민소서무면역치료작용.
Objective The incidence of food allergy has increased in recent years and there is no effective way to treat it except strict dietary avoidance and rapid medical treatment in case of accidental exposure.Oral tolerance,as a new method,has shown great promise as an alternative approach to prevention and treatment for allergic disease.It was reported that all-trans retinoic acid (atRA) plays an important role in inducing oral tolerance in vitro.Our study aimed to investigate the immunological effect of different doses of atRA on ovalbumin (OVA) allergic BALB/c mice.Method BALB/c mice were sensitized by intraperitoneal injection with OVA to establish allergic animal model.According to the dose of atRA given,40 OVA allergic BALB/c mice were divided into 4 groups:the mice in high dose group were treated with 100 mg/kg atRA (atRA-H),those in median dose group were treated with 50 mg/kg atRA (atRA-M),those in low dose group were treated with 20 mg/kg atRA (atRA-L) and the mice in control group were given vehicle-soy oil only (CTR).After 12 days of atRA intervention,weight was measured,the mice were checked for diarrhea,and intestinal histology was observed after hematoxylin and eosin staining.The level of OVA-IgE in serum,total IgA and OVA-IgA in feces were measured by ELISA.The percentage of CD4 +CD25 + FoxP3 + T cells in CD4 + T cells in mesenteric lymph node was detected by flow cytometry.Result Compared with that of CTR group,the level of OVA-IgE in serum (1.221 ±0.367 vs.0.793 ±0.616) and OVA-IgA (1.573 ± 0.656 vs.0.905 ± 0.279) in feces decreased significantly (P =0.006 and 0.012,respectively) without weight and intestinal histology changes after low dose of atRA administration.However,there was no significant difference in the percentage of CD4 + CD25 + FoxP3 + T cells in CD4 + T cells in mesenteric lymph node (10.641 ± 1.218 vs.10.936 ± 0.954) between atRA-L and CTR group (P ＞ 0.05).While in animals with high and median dose of atRA administration,no immunologic improvement was found,instead,there was weight loss and intestinal mucosal damage.Conclusion Low dose of atRA intervention seems to induce immune suppression in vivo resulting in positive effccts on OVA allergic mice.However,median and high dose atRA had no therapeutic effect on OVA allergic mice.
