CAJ | 학술논문

目的探讨滤泡辅助性T细胞(Tfh)在系统性红斑狼疮(SLE)患者外周血中百分率的变化及意义.方法采用流式细胞术检测60例SLE患者(其中活动期患者30例,稳定期患者30例)和30名健康体检者(对照组)外周血中CD4+CXCR5+ICOS+ Tfh细胞的百分率及Tfh细胞的活化标志物CD69的表达百分率.比较SLE患者外周血Tfh细胞百分率与SLE疾病活动性指数(SLEDAI)、抗核抗体滴度及补体C3、C4水平之间的相关性.统计学处理采用单因素方差分析和Pearson相关分析.结果 SLE活动组外周血Tfh细胞百分率[(0.80±0.17)％]显著高于SLE稳定组[(0.63±0.13)％]及健康对照组[(0.57±0.08)％](P均＜0.01),SLE稳定组亦高于健康对照组(P＜0.05).SLE活动组外周血Tfh细胞CD69的表达百分率[(7.3±1.6)％]显著高于SLE稳定组[(5.9±1.3)％]及健康对照组[(5.2±0.9)％](P均＜0.01),SLE稳定组与健康对照组差异无统计学意义(P＞0.05).SLE患者外周血Tfh细胞百分率与SLEDAI呈正相关(r=0.680,P＜0.01),与补体C3水平呈负相关(r=-0.416,P＜0.05),与ANA滴度及补体C4水平无明显相关性(r=-0.042,r=-0.204,P均＞0.05).结论 SLE患者外周血中Tfh细胞百分率增多及活性增强可能与SLE的发生及发展有关.
목적 탐토려포보조성T세포(Tfh)재계통성홍반랑창(SLE)환자외주혈중백분솔적변화급의의.방법 채용류식세포술검측60례SLE환자(기중활동기환자30례,은정기환자30례)화30명건강체검자(대조조)외주혈중CD4+CXCR5+ICOS+ Tfh세포적백분솔급Tfh세포적활화표지물CD69적표체백분솔.비교SLE환자외주혈Tfh세포백분솔여SLE질병활동성지수(SLEDAI)、항핵항체적도급보체C3、C4수평지간적상관성.통계학처리채용단인소방차분석화Pearson상관분석.결과 SLE활동조외주혈Tfh세포백분솔[(0.80±0.17)％]현저고우SLE은정조[(0.63±0.13)％]급건강대조조[(0.57±0.08)％](P균＜0.01),SLE은정조역고우건강대조조(P＜0.05).SLE활동조외주혈Tfh세포CD69적표체백분솔[(7.3±1.6)％]현저고우SLE은정조[(5.9±1.3)％]급건강대조조[(5.2±0.9)％](P균＜0.01),SLE은정조여건강대조조차이무통계학의의(P＞0.05).SLE환자외주혈Tfh세포백분솔여SLEDAI정정상관(r=0.680,P＜0.01),여보체C3수평정부상관(r=-0.416,P＜0.05),여ANA적도급보체C4수평무명현상관성(r=-0.042,r=-0.204,P균＞0.05).결론 SLE환자외주혈중Tfh세포백분솔증다급활성증강가능여SLE적발생급발전유관.
Objective To investigate the change and significance of T follicular helper cells(Tfh) in the peripheral blood of patients with systemic lupus erythematosus (SLE).Methods The percentage of CD4+ CXCR5+ICOS+ Tfh cells and the expression of activation marker CD69 on the Tfh cells of peripheral blood from 60 SLE patients (30 in active and 30 in inactive) and 30 healthy subjects (control group) were determined by flow cytometry.The correlations between the percentage of Tfh cells of SLE patients and SLE disease activity index (SLEDAI),anti-nuclear antibody (ANA) titers and the levels of complement 3 (C3),complement 4 (C4) were analyzed.ANOVA and Pearson's correlation analysis were used for statistical analysis.Results The percentage of the Tfh cells in the peripheral blood of active SLE patients was higher than inactive patients and healthy controls [(0.80±0.17)％ vs (0.63±0.13)％ vs (0.57±0.08)％; P＜0.01],there was also statistical significance between inactive patients and healthy controls (P＜0.05).The expression of CD69 on the Tfh cells in the peripheral blood of active SLE patients was higher than that in inactive and healthy controls [(7.3±1.6)％ vs (5.9±1.3)％ vs (5.2±0.9)％; P＜0.01].There was no statistical significant difference between inactive patients and healthy controls (P＞0.05).The percentage of Tfh cells of SLE patients was significantly related with SLEDAI (r=0.680,P＜0.01) and C3 levels (r=-0.416,P＜0.05),butthere was no correlation between that and the ANA titer,C4 levels (r=-0.042,-0.204,P＞0.05).Conclusion Increased percentage and activity of the Tfh cells in the peripheral blood of patients might contribute to the pathogenesis and development of SLE.