中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2013年
7期
481-484
,共4页
脊柱炎,强直性%T淋巴细胞,辅助诱导%T淋巴细胞
脊柱炎,彊直性%T淋巴細胞,輔助誘導%T淋巴細胞
척주염,강직성%T림파세포,보조유도%T림파세포
Spondylitis,ankylosing%T-lymphocytes,helper-inducer%T-lymphocytes
目的 分析强直性脊柱炎(AS)患者外周血T辅助细胞17(T help cell 17,Th17)和调节性T细胞的比例变化与疾病状态的关系,初步阐明Th17/调节性T细胞在AS发病机制中的作用.方法 用流式细胞术检测40例AS患者和20名健康对照外周血Th17和Foxp3+CD4+CD25+调节性T细胞的比例.酶联免疫吸附法检测AS患者及健康对照组血清中白细胞介素(IL)-2、IL-10、转化生长因子(TGF)-β、IL-6、IL-17、IL-23的浓度并分析这些细胞因子含量与传统指标的相关性.所用的统计学方法有t检验、单因素方差分析、SNK-q检验和Spearman相关性分析.结果 AS患者外周血Th17细胞百分率明显高于健康对照组(P＜0.05),且活动组AS患者外周血Th17细胞[(2.89±1.77)％]也明显高于稳定组[(2.10±0.82)％] AS(P＜0.05),而活动组AS患者外周血调节性T细胞的百分率[(3.9±1.2)％]明显低于健康对照组[(4.9±1.2)％](P＜0.05);AS活动组Th17/调节性T细胞比值明显高于健康对照组及AS稳定组(P＜0.05).AS患者外周血血清中IL-17和IL-23[(13±4)和(2998±1009) ng/L]的浓度明显高于健康对照组[(9±3)和2356±666)ng/L] (t=2.203,P=0.032和t=2.088,P=0.041);活动组AS患者IL-17和IL-23含量[(16±5)和(3102±999) ng/L]明显高于稳定组[(10±4)和(2390±614) nig/L] AS患者(t=2.808,P=0.008和t=2.424,P=0.020).相关性分析显示除了IL-17与Bath强直性脊柱炎病情活动指数(BASDAI)呈正相关(r=0.32,P=0.044)外,其余各指标间均无相关性.结论 Th17细胞百分率的增加而调节性T细胞百分率的降低可能与AS的发生、发展有关.通过调节Th17/节性T细胞的平衡,有可能为AS的治疗提供一条新思路.
目的 分析彊直性脊柱炎(AS)患者外週血T輔助細胞17(T help cell 17,Th17)和調節性T細胞的比例變化與疾病狀態的關繫,初步闡明Th17/調節性T細胞在AS髮病機製中的作用.方法 用流式細胞術檢測40例AS患者和20名健康對照外週血Th17和Foxp3+CD4+CD25+調節性T細胞的比例.酶聯免疫吸附法檢測AS患者及健康對照組血清中白細胞介素(IL)-2、IL-10、轉化生長因子(TGF)-β、IL-6、IL-17、IL-23的濃度併分析這些細胞因子含量與傳統指標的相關性.所用的統計學方法有t檢驗、單因素方差分析、SNK-q檢驗和Spearman相關性分析.結果 AS患者外週血Th17細胞百分率明顯高于健康對照組(P＜0.05),且活動組AS患者外週血Th17細胞[(2.89±1.77)％]也明顯高于穩定組[(2.10±0.82)％] AS(P＜0.05),而活動組AS患者外週血調節性T細胞的百分率[(3.9±1.2)％]明顯低于健康對照組[(4.9±1.2)％](P＜0.05);AS活動組Th17/調節性T細胞比值明顯高于健康對照組及AS穩定組(P＜0.05).AS患者外週血血清中IL-17和IL-23[(13±4)和(2998±1009) ng/L]的濃度明顯高于健康對照組[(9±3)和2356±666)ng/L] (t=2.203,P=0.032和t=2.088,P=0.041);活動組AS患者IL-17和IL-23含量[(16±5)和(3102±999) ng/L]明顯高于穩定組[(10±4)和(2390±614) nig/L] AS患者(t=2.808,P=0.008和t=2.424,P=0.020).相關性分析顯示除瞭IL-17與Bath彊直性脊柱炎病情活動指數(BASDAI)呈正相關(r=0.32,P=0.044)外,其餘各指標間均無相關性.結論 Th17細胞百分率的增加而調節性T細胞百分率的降低可能與AS的髮生、髮展有關.通過調節Th17/節性T細胞的平衡,有可能為AS的治療提供一條新思路.
목적 분석강직성척주염(AS)환자외주혈T보조세포17(T help cell 17,Th17)화조절성T세포적비례변화여질병상태적관계,초보천명Th17/조절성T세포재AS발병궤제중적작용.방법 용류식세포술검측40례AS환자화20명건강대조외주혈Th17화Foxp3+CD4+CD25+조절성T세포적비례.매련면역흡부법검측AS환자급건강대조조혈청중백세포개소(IL)-2、IL-10、전화생장인자(TGF)-β、IL-6、IL-17、IL-23적농도병분석저사세포인자함량여전통지표적상관성.소용적통계학방법유t검험、단인소방차분석、SNK-q검험화Spearman상관성분석.결과 AS환자외주혈Th17세포백분솔명현고우건강대조조(P＜0.05),차활동조AS환자외주혈Th17세포[(2.89±1.77)％]야명현고우은정조[(2.10±0.82)％] AS(P＜0.05),이활동조AS환자외주혈조절성T세포적백분솔[(3.9±1.2)％]명현저우건강대조조[(4.9±1.2)％](P＜0.05);AS활동조Th17/조절성T세포비치명현고우건강대조조급AS은정조(P＜0.05).AS환자외주혈혈청중IL-17화IL-23[(13±4)화(2998±1009) ng/L]적농도명현고우건강대조조[(9±3)화2356±666)ng/L] (t=2.203,P=0.032화t=2.088,P=0.041);활동조AS환자IL-17화IL-23함량[(16±5)화(3102±999) ng/L]명현고우은정조[(10±4)화(2390±614) nig/L] AS환자(t=2.808,P=0.008화t=2.424,P=0.020).상관성분석현시제료IL-17여Bath강직성척주염병정활동지수(BASDAI)정정상관(r=0.32,P=0.044)외,기여각지표간균무상관성.결론 Th17세포백분솔적증가이조절성T세포백분솔적강저가능여AS적발생、발전유관.통과조절Th17/절성T세포적평형,유가능위AS적치료제공일조신사로.
Objective To analyze the variation of peripheral blood Th17 cells and Foxp3+CD4+CD25+regulatory T (Treg)cells in patients with ankylosing spondylitis (AS),and to interpret the role of the ratio imbalance of peripheral blood Th17/Treg cells in the pathogenesis of AS.Methods The percentage of peripheral blood Th17 cells and Foxp3 +CD4+CD25+ Treg cells in AS patients and healthy subjects were determined by flow cytometry (FCM).The concentrations of IL-2,IL-10,TGF-β,IL-6,IL-17 and IL-23 in the serum were measured by enzyme linked immunosorbent assay.The correlation between these cytokines and traditional parameters was analyzed.T test,ANOVA,SNK-q and Speannan's analysis were used for statistical analysis.Results Compared with healthy controls,the percentage of Th17 cells in AS patients increased significantly (P＜0.05); Compared with the inactive AS patients,the percentage of Th17 cells in active AS patients increased significantly [(2.10±0.82)％,(2.89±1.77)％,P＜0.05)]; the percentage of Foxp3+CD4+CD25+ Treg cells in active AS patients was markedly lower than healthy controls (P＜0.05).Compared with healthy controls and inactive AS patients,the specific value of Th17/Treg in active AS patients increased significantly(P＜0.05).The concentrations of IL-17 and IL-23 was found significantly higher in patients with AS than that in healthy controls (t=2.203,P=0.032 and t=2.088,P=0.041).The concentrations of IL-17 and IL-23 was found significantly higher in patients with active AS than that in inactive AS (t=2.808,P=0.008 and t=2.242,P=0.020).The correlation analysis revealed that these cytokines and traditional parameters was not relevant except IL-17/BASDAI (r=0.32,P=0.044).Conclusion The increased ratio of Th17 cells and decreased ratio of Foxp3+CD4+CD25+ Treg cells may play a critical role in AS progression.Manipulating the balance of Th17/Treg would provide a new approach for the treatment of AS.
