中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2013年
9期
601-605
,共5页
尹薇%丁艳%刘凡%何学莲%康世秀%曾小燕
尹薇%丁豔%劉凡%何學蓮%康世秀%曾小燕
윤미%정염%류범%하학련%강세수%증소연
关节炎,幼年型类风湿%间质胶原酶%基质溶解素1%金属蛋白酶类组织抑制剂%白细胞介素1%肿瘤坏死因子α%转化生长因子β
關節炎,幼年型類風濕%間質膠原酶%基質溶解素1%金屬蛋白酶類組織抑製劑%白細胞介素1%腫瘤壞死因子α%轉化生長因子β
관절염,유년형류풍습%간질효원매%기질용해소1%금속단백매류조직억제제%백세포개소1%종류배사인자α%전화생장인자β
Arthritis,juvenile rheumatoid%Interstitial collagenase%Stromelysin 1%Tissue inhibitor of metalloproteinases%Interleukin-1%Tumor necrosis factor-alpha%Transforming growth factor beta
目的 探讨幼年特发性关节炎(JIA)多关节型患儿血清及关节液基质金属蛋白酶(MMP)-I、MMP-3、金属蛋白酶抑制剂(TIMP)-1、白细胞介素(IL)-1、肿瘤坏死因子(TNF)-α,转化生长因子(TGF)-β水平的变化及临床意义.方法 酶联免疫吸附试验(ELISA)检测32例多关节型JIA患儿及28例对照组(外伤性关节炎患儿)血清及关节液中MMP-1、MMP-3、TIMP-1、IL-1、TNF-α、TGF-β的水平.同时检测血白细胞计数、C反应蛋白(CRP)、红细胞沉降率(ESR)及类风湿因子(RF)水平.统计学方法采用两个独立样本的t检验及Pearson直线相关分析.结果 ①JIA组血清MMP-1、MMP-3、IL-1、TNF-α水平分别为(158±67) ng/ml、(212±89) ng/ml、(39±19) pg/ml、(26±10) pg/ml,明显高于对照组(P均＜0.05),MMP-3/TIMP-1为0.86±0.32,亦较对照组升高(P＜0.05),TIMP-1、TGF-β水平分别为(248±88)、(17±9)ng/ml,明显低于对照组(P均＜0.05)；血清MMP-3、MMP-3/TlMP-1与白细胞计数、CRP、ESR呈正相关(P均＜0.05).②JIA组关节液MMP-1、MMP-3、IL-1、TNF-α水平分别为(216±78) ng/ml、(766±291) ng/ml、(56±21) pg/ml、(36±14) pg/ml,明显高于对照组(P均＜0.05),MMP-3/TIMP-1为2.68±0.89,亦较对照组明显升高(P＜0.05),TIMP-1、TGF-β水平分别为(286±88)、(12±4) ng/ml,明显低于对照组(P均＜0.05).③JIA组关节液MMP-1、MMP-3、TIMP-1、IL-1、TNF-α水平明显高于其血清水平(P均＜0.05),TGF-β水平则明显低于其血清水平(P＜0.05).结论 血清及关节液中MMP-1、MMP-3、IL-1、TNF-α水平升高,TIMP-1永平降低,参与了JIA多关节型的发病过程,TGF-β水平降低,在其发病中可能起保护作用.血清MMP-3/ⅡMP-1比值与JIA炎性指标呈正相关,有助于判断病情活动性.
目的 探討幼年特髮性關節炎(JIA)多關節型患兒血清及關節液基質金屬蛋白酶(MMP)-I、MMP-3、金屬蛋白酶抑製劑(TIMP)-1、白細胞介素(IL)-1、腫瘤壞死因子(TNF)-α,轉化生長因子(TGF)-β水平的變化及臨床意義.方法 酶聯免疫吸附試驗(ELISA)檢測32例多關節型JIA患兒及28例對照組(外傷性關節炎患兒)血清及關節液中MMP-1、MMP-3、TIMP-1、IL-1、TNF-α、TGF-β的水平.同時檢測血白細胞計數、C反應蛋白(CRP)、紅細胞沉降率(ESR)及類風濕因子(RF)水平.統計學方法採用兩箇獨立樣本的t檢驗及Pearson直線相關分析.結果 ①JIA組血清MMP-1、MMP-3、IL-1、TNF-α水平分彆為(158±67) ng/ml、(212±89) ng/ml、(39±19) pg/ml、(26±10) pg/ml,明顯高于對照組(P均＜0.05),MMP-3/TIMP-1為0.86±0.32,亦較對照組升高(P＜0.05),TIMP-1、TGF-β水平分彆為(248±88)、(17±9)ng/ml,明顯低于對照組(P均＜0.05)；血清MMP-3、MMP-3/TlMP-1與白細胞計數、CRP、ESR呈正相關(P均＜0.05).②JIA組關節液MMP-1、MMP-3、IL-1、TNF-α水平分彆為(216±78) ng/ml、(766±291) ng/ml、(56±21) pg/ml、(36±14) pg/ml,明顯高于對照組(P均＜0.05),MMP-3/TIMP-1為2.68±0.89,亦較對照組明顯升高(P＜0.05),TIMP-1、TGF-β水平分彆為(286±88)、(12±4) ng/ml,明顯低于對照組(P均＜0.05).③JIA組關節液MMP-1、MMP-3、TIMP-1、IL-1、TNF-α水平明顯高于其血清水平(P均＜0.05),TGF-β水平則明顯低于其血清水平(P＜0.05).結論 血清及關節液中MMP-1、MMP-3、IL-1、TNF-α水平升高,TIMP-1永平降低,參與瞭JIA多關節型的髮病過程,TGF-β水平降低,在其髮病中可能起保護作用.血清MMP-3/ⅡMP-1比值與JIA炎性指標呈正相關,有助于判斷病情活動性.
목적 탐토유년특발성관절염(JIA)다관절형환인혈청급관절액기질금속단백매(MMP)-I、MMP-3、금속단백매억제제(TIMP)-1、백세포개소(IL)-1、종류배사인자(TNF)-α,전화생장인자(TGF)-β수평적변화급림상의의.방법 매련면역흡부시험(ELISA)검측32례다관절형JIA환인급28례대조조(외상성관절염환인)혈청급관절액중MMP-1、MMP-3、TIMP-1、IL-1、TNF-α、TGF-β적수평.동시검측혈백세포계수、C반응단백(CRP)、홍세포침강솔(ESR)급류풍습인자(RF)수평.통계학방법채용량개독립양본적t검험급Pearson직선상관분석.결과 ①JIA조혈청MMP-1、MMP-3、IL-1、TNF-α수평분별위(158±67) ng/ml、(212±89) ng/ml、(39±19) pg/ml、(26±10) pg/ml,명현고우대조조(P균＜0.05),MMP-3/TIMP-1위0.86±0.32,역교대조조승고(P＜0.05),TIMP-1、TGF-β수평분별위(248±88)、(17±9)ng/ml,명현저우대조조(P균＜0.05)；혈청MMP-3、MMP-3/TlMP-1여백세포계수、CRP、ESR정정상관(P균＜0.05).②JIA조관절액MMP-1、MMP-3、IL-1、TNF-α수평분별위(216±78) ng/ml、(766±291) ng/ml、(56±21) pg/ml、(36±14) pg/ml,명현고우대조조(P균＜0.05),MMP-3/TIMP-1위2.68±0.89,역교대조조명현승고(P＜0.05),TIMP-1、TGF-β수평분별위(286±88)、(12±4) ng/ml,명현저우대조조(P균＜0.05).③JIA조관절액MMP-1、MMP-3、TIMP-1、IL-1、TNF-α수평명현고우기혈청수평(P균＜0.05),TGF-β수평칙명현저우기혈청수평(P＜0.05).결론 혈청급관절액중MMP-1、MMP-3、IL-1、TNF-α수평승고,TIMP-1영평강저,삼여료JIA다관절형적발병과정,TGF-β수평강저,재기발병중가능기보호작용.혈청MMP-3/ⅡMP-1비치여JIA염성지표정정상관,유조우판단병정활동성.
Objective The purpose of the study was to investigate the changes and clinical significance of matrix metalloproteinase (MMP)-1,MMP-3,tissue inhibitor of metalloproteinase (TIMP),interleukin (IL)-1,tumor necrosis factor (TNF)-α and transforming growth factor(TGF)-β in juvenile idiopathic arthritis (JIA).Methods Thirty-two JIA subjects and 28 controls (traumatic arthritis patients) were included into this study.The MMP-1,MMP-3,TIMP,IL-1,TNF-α and TGF-β level in the serum and synovia were assessed by ELISA.The WBC count,the level of CRP,ESR,RF were also detected.Independent t-test and Pearson's analysis were adopted for data analysis.Results ① The level of MMP-1,MMP-3,IL-1 and TNF-α in the serum was (158±67) ng/ml,(212±89) ng/ml,(39±19) pg/ml,(26±10) pg/ml respectively,which was significantly higher than that of the control group (all P＜0.05) ; the ratio of MMP-3/TIMP-1 (0.86±0.32) was higher in the study group than that of the control group (P＜0.05),while the value of TIMP-1,TGF-β was (248±88),(17±9) ng/ml respectively,which was lower than that of the control group (P＜0.05).The value of seral MMP-3,MMP-3/TIMP-1 was positively correlated with that of WBC,CRP,ESR in the JIA group (all P＜0.05).② The value of MMP-1,MMP-3,IL-1 and TNF-α in the synovia was (216±78) ng/nl,(766±291)ng/ml,(56±21) pg/ml,(36±14) pg/ml respectively,which was higher than that of the control group (all P＜0.05); the ratio of MMP-3/TlMP-1 (2.68±0.89) was higher than that of the control group (P＜0.05),while the value of TIMP-1,TGF-β was (286±88) ng/ml,(12±4) ng/ml respectively,which was lower than that of the control group (all P＜0.05).③ The value of MMP-1,MMP-3,TIMP-1,IL-1 and TNF-α in the synovia was higher than that in the serum (all P＜0.05),while the value of TGF-β was lower than that in the serum (P＜0.05).Conclusion The value of MMP-1,MMP-3,IL-1 and TNF-α increases both in the serum and synovia,while the value of TIMP-1 decreases.The value of TGF-β decreases,which may have protective effect on JIA.The ratio of MMP-3/TIMP-1 in the serum is positively correlated to inflammation parameters,which may be used to judge the activity of illness in JIA.
