中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2013年
10期
692-695
,共4页
庄子云%刘斌%揣瑞梅%徐龙强%李长贵%刘世国
莊子雲%劉斌%揣瑞梅%徐龍彊%李長貴%劉世國
장자운%류빈%췌서매%서룡강%리장귀%류세국
巨噬细胞游走抑制因子%多态性,单核苷酸%痛风
巨噬細胞遊走抑製因子%多態性,單覈苷痠%痛風
거서세포유주억제인자%다태성,단핵감산%통풍
Macrophage migration inhibitory factors%Polymorphism,single nucleotide%Gout
目的 探讨中国汉族男性人群巨噬细胞移动抑制因子(MIF)启动子区-173G/C(rs755622)基因多态性的分布与原发性痛风遗传易感性之间的关系.方法 选取380例痛风患者和378名健康对照者,采用聚合酶链反应-序列特异性引物(PCR-SSP)技术,检测中国汉族男性人群中MIF启动子区-173G/C基因等位基因及基因型频率的遗传分布,Hardy-Weinberg检验确认标本的群体代表性后,采用x2检验进行关联分析,采用t检验对MIF基因-173G/C位点基因多态性与痛风危险因素进行比较.结果 痛风组MIF启动子区-173位点GG、GC、CC基因型的频率为62.1％(236个)、34.2％(130个)、3.7％(14个)；健康对照组MIF启动子区-173位点GG、GC、CC基因型频率为66.5％(252个)、29.8％(113个)、3.7％(14个),2组之间比较差异无统计学意义(x2=1.713,P=0.425).痛风组等位基因G、C的频率分别为79.2％(602个)和20.8％(158个),健康对照组为81.4％(617个)和18.6％(141个),2组之间等位基因G、C频率的比较差异也无统计学意义(x2=1.148,P=0.302).将痛风组基因型分析为GG基因型与GC基因型合并为GG+GC组,结果显示:GG+GC组与CC组之间年龄[(51±13)和(50±15)岁,t=0.369,P=0.712]、血糖[(7.1±8.8)和(6.1±1.2)mmol/L,t=0.352,P=0.725]、甘油三酯[(2.3±1.6)和(2.9±3.4)mmol/L,t=-1.207,P=0.228]、胆固醇[(5.3±1.2)和(5.7±1.4)mmol/L,t=-1.207,P=0.228]、尿素氮[(5.8±2.9)和(6.2±2.2)mmol/L,t=-0.513,P=0.608]、肌酐[(92±52)和(84±17)μmol/L,t=0.537,P=0.592]、尿酸[(472±103)和(557±154)μmol/L,t=-2.949,P=0.03]比较差异均无统计学意义(均P＞0.05).经t检验,MIF基因-173G/C基因多态性位点与痛风病危险因素之间无相关性(P＞0.05).结论 中国汉族男性人群中MIF基因启动子区-173G/C基因多态性与痛风易感性之间没有关联性.
目的 探討中國漢族男性人群巨噬細胞移動抑製因子(MIF)啟動子區-173G/C(rs755622)基因多態性的分佈與原髮性痛風遺傳易感性之間的關繫.方法 選取380例痛風患者和378名健康對照者,採用聚閤酶鏈反應-序列特異性引物(PCR-SSP)技術,檢測中國漢族男性人群中MIF啟動子區-173G/C基因等位基因及基因型頻率的遺傳分佈,Hardy-Weinberg檢驗確認標本的群體代錶性後,採用x2檢驗進行關聯分析,採用t檢驗對MIF基因-173G/C位點基因多態性與痛風危險因素進行比較.結果 痛風組MIF啟動子區-173位點GG、GC、CC基因型的頻率為62.1％(236箇)、34.2％(130箇)、3.7％(14箇)；健康對照組MIF啟動子區-173位點GG、GC、CC基因型頻率為66.5％(252箇)、29.8％(113箇)、3.7％(14箇),2組之間比較差異無統計學意義(x2=1.713,P=0.425).痛風組等位基因G、C的頻率分彆為79.2％(602箇)和20.8％(158箇),健康對照組為81.4％(617箇)和18.6％(141箇),2組之間等位基因G、C頻率的比較差異也無統計學意義(x2=1.148,P=0.302).將痛風組基因型分析為GG基因型與GC基因型閤併為GG+GC組,結果顯示:GG+GC組與CC組之間年齡[(51±13)和(50±15)歲,t=0.369,P=0.712]、血糖[(7.1±8.8)和(6.1±1.2)mmol/L,t=0.352,P=0.725]、甘油三酯[(2.3±1.6)和(2.9±3.4)mmol/L,t=-1.207,P=0.228]、膽固醇[(5.3±1.2)和(5.7±1.4)mmol/L,t=-1.207,P=0.228]、尿素氮[(5.8±2.9)和(6.2±2.2)mmol/L,t=-0.513,P=0.608]、肌酐[(92±52)和(84±17)μmol/L,t=0.537,P=0.592]、尿痠[(472±103)和(557±154)μmol/L,t=-2.949,P=0.03]比較差異均無統計學意義(均P＞0.05).經t檢驗,MIF基因-173G/C基因多態性位點與痛風病危險因素之間無相關性(P＞0.05).結論 中國漢族男性人群中MIF基因啟動子區-173G/C基因多態性與痛風易感性之間沒有關聯性.
목적 탐토중국한족남성인군거서세포이동억제인자(MIF)계동자구-173G/C(rs755622)기인다태성적분포여원발성통풍유전역감성지간적관계.방법 선취380례통풍환자화378명건강대조자,채용취합매련반응-서렬특이성인물(PCR-SSP)기술,검측중국한족남성인군중MIF계동자구-173G/C기인등위기인급기인형빈솔적유전분포,Hardy-Weinberg검험학인표본적군체대표성후,채용x2검험진행관련분석,채용t검험대MIF기인-173G/C위점기인다태성여통풍위험인소진행비교.결과 통풍조MIF계동자구-173위점GG、GC、CC기인형적빈솔위62.1％(236개)、34.2％(130개)、3.7％(14개)；건강대조조MIF계동자구-173위점GG、GC、CC기인형빈솔위66.5％(252개)、29.8％(113개)、3.7％(14개),2조지간비교차이무통계학의의(x2=1.713,P=0.425).통풍조등위기인G、C적빈솔분별위79.2％(602개)화20.8％(158개),건강대조조위81.4％(617개)화18.6％(141개),2조지간등위기인G、C빈솔적비교차이야무통계학의의(x2=1.148,P=0.302).장통풍조기인형분석위GG기인형여GC기인형합병위GG+GC조,결과현시:GG+GC조여CC조지간년령[(51±13)화(50±15)세,t=0.369,P=0.712]、혈당[(7.1±8.8)화(6.1±1.2)mmol/L,t=0.352,P=0.725]、감유삼지[(2.3±1.6)화(2.9±3.4)mmol/L,t=-1.207,P=0.228]、담고순[(5.3±1.2)화(5.7±1.4)mmol/L,t=-1.207,P=0.228]、뇨소담[(5.8±2.9)화(6.2±2.2)mmol/L,t=-0.513,P=0.608]、기항[(92±52)화(84±17)μmol/L,t=0.537,P=0.592]、뇨산[(472±103)화(557±154)μmol/L,t=-2.949,P=0.03]비교차이균무통계학의의(균P＞0.05).경t검험,MIF기인-173G/C기인다태성위점여통풍병위험인소지간무상관성(P＞0.05).결론 중국한족남성인군중MIF기인계동자구-173G/C기인다태성여통풍역감성지간몰유관련성.
Objective To explore gene polymorphism of the G/C genotype of-173G/C(rs755622)in the promoter of macrophage migration inhibitory factor(MIF)gene in male population,and thus to investigate the relationship between gene polymorphism of MIF and gout.Methods A total of 380 gout patients and 378 healthy controls were enrolled.The possible association between the polymorphism of MIF-173G/C and gout in Chinese were investigated and genotype frequencies and allelic frequencies were analyzed by polymerase chain reaction with sequence-specific primers(PCR-SSP)method.Hardy-Weinberg was used to verify the representativeness of the samples.Comparisons between the groups were performed with x2 test.The gene polymorphism of MIF and gout was performed by t test.Results The frequencies of GG,GC,CC genotypes were 62.1％(236 cases),34.2％(130 cases)and 3.7％(14 cases),respectively among gout patients,while they were 66.5％(252 cases),29.8％(113 cases)and 3.7％(14 cases),respectively among the controls.There was no statistical difference in MIF-173G/C genotype frequencies between gout patients and controls(x2=1.713,P=0.425).The allele frequencies of G and C in gout cases were 79.2％(602 cases)and 20.8％(158cases),while the controls were 81.4％(617 cases)and 18.6％(141 cases),and no significant difference between them could be found(x2=1.148,P=0.302).Combine GG and GC of gout into GG+GC,the association analysis of the two groups showed that,mean age,leves of glucose,TG,TC,BUN,Cr and UA of the GG+GC group and the CC group were(51±13)and(50±15)t=0.369,P=0.712;(7.1±8.8)and(6.1±1.2)mmol/L,t=0.352,P=0.725;(2.3±1.6)and(2.9±3.4)mmol/L,t=-1.207,P=0.228;(5.3±1.2)and(5.7±1.4)mmol/L,t=-1.207,P=0.228;(5.8±2.9)and(6.2±2.2)mmol/L,t=-0.513,P=0.608;(92±52)and(84±17)μmol/L,t=0.537,P=0.592;(472±103)vs(557±154)μmol/L,t=-2.949,P=0.03 respectively;no significant difference was found in the two group.Moreover,no association between MIF-173G/C genotypes and risk factors for gout were detected in gout cases by t-test.Conclusion Results of the present study suggest that the G/C genotype of-173G/C in the promoter of MIF gene is not associated with gout in male population.
