CAJ | 학술논문

目的探讨白细胞介素(IL)-27在干燥综合征(SS)患者血清表达水平及其对T细胞来源IL-10的调节.方法采用酶联免疫吸附试验(ELISA)检测34例SS患者与33名健康对照者血清IL-27和IL-10水平.采集SS患者外周血,淋巴细胞分离液分选外周血单个核细胞,分成2组,分别给予植物血凝素(PHA)或PHA+IL-27刺激,72 h后收集细胞,流式细胞术检测CD3+CD8-IL-10+细胞百分率,实时荧光定量聚合酶链反应术检测IL-10转录因子c-Maf mRNA表达.应用配对t检验比较2组细胞百分率及mRNA表达差异,应用t检验比较2组IL-27水平差异,选用Pearson相关分析及多元线性回归分析统计IL-27与IL-10及临床指标的关系.结果 SS患者血清IL-27水平[(158±34) pg/ml]显著高于健康对照者[(139±18) pg/ml,t=2.823,P＜0.01],与IL-10、IgG水平呈正相关(r=0.384,P＜0.05；r=0.354,P＜0.05),与SS疾病活动度评分(SSDAI)呈负相关(r=-0.503,P＜0.01),多元线性回归分析显示IL-27与IL-10、SSDAI有线性关系(β=0.412,P＜0.01；β=-0.525,P＜0.01).PHA+IL-27刺激组较PHA刺激组CD3+CD8-IL-10+细胞百分率增高(P＜0.01,14例),c-Maf mRNA水平也增高(P＜0.01,22例).结论 IL-27可促进CD3+CD8-T细胞表达抑炎性细胞因子IL-10,参与对SS病情调控.
목적 탐토백세포개소(IL)-27재간조종합정(SS)환자혈청표체수평급기대T세포래원IL-10적조절.방법 채용매련면역흡부시험(ELISA)검측34례SS환자여33명건강대조자혈청IL-27화IL-10수평.채집SS환자외주혈,림파세포분리액분선외주혈단개핵세포,분성2조,분별급여식물혈응소(PHA)혹PHA+IL-27자격,72 h후수집세포,류식세포술검측CD3+CD8-IL-10+세포백분솔,실시형광정량취합매련반응술검측IL-10전록인자c-Maf mRNA표체.응용배대t검험비교2조세포백분솔급mRNA표체차이,응용t검험비교2조IL-27수평차이,선용Pearson상관분석급다원선성회귀분석통계IL-27여IL-10급림상지표적관계.결과 SS환자혈청IL-27수평[(158±34) pg/ml]현저고우건강대조자[(139±18) pg/ml,t=2.823,P＜0.01],여IL-10、IgG수평정정상관(r=0.384,P＜0.05；r=0.354,P＜0.05),여SS질병활동도평분(SSDAI)정부상관(r=-0.503,P＜0.01),다원선성회귀분석현시IL-27여IL-10、SSDAI유선성관계(β=0.412,P＜0.01；β=-0.525,P＜0.01).PHA+IL-27자격조교PHA자격조CD3+CD8-IL-10+세포백분솔증고(P＜0.01,14례),c-Maf mRNA수평야증고(P＜0.01,22례).결론 IL-27가촉진CD3+CD8-T세포표체억염성세포인자IL-10,삼여대SS병정조공.
Objective To measure the level of interleukin (IL)-27 in sera of patients with Sj(o)gren's syndrome (SS) and investigate its role in IL-10 in CD3+CD8-T cells.Methods Serum levels of 1L-27 and IL-10 from 34 SS patients and 33 healthy controls were tested by enzyme linked immunosorbent assay (ELISA).Peripheral blood monocytes (PBMCs) from SS patients were collected and cultured in different conditions (one with PHA,the other with PHA and IL-27).Seventy-two hours later,cells were harvested and the percentages of CD3 +CD8-IL-10+ cells were measured by flow cytometry.Expressions of c-Maf,IL-10 transcription factor,were examined using real-time polymerase chain reaction (PCR).The data were analyzed by t test,Pearson's correlation analysis and multiple linear regression analysis.Results Serum level of IL-27 in patients with SS [(158±34) pg/ml] was significantly higher than that in healthy controls [(139±18) pg/ml,t=2.823,P＜0.01].IL-27 level was positively correlated with IL-10 and IgG levels (r=0.384,P＜0.05; r=0.354,P＜0.05),but negatively correlated with SSDAI (r=0.503,P＜0.01).Multiple regression analysis showed independent correlation of IL-27 with IL-10 and SSDAI in SS patients (β=0.412,P＜0.01; β=-0.525,P＜0.01),but not IgG.CD3+CD8-IL-10+ cells was upregulated by IL-27 in vitro (P＜0.01,n=14),so was c-Maf (P＜0.01,n=22).Conclusion IL-27 could ameliorate disease activity of Sjogren's syndrome.