中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2013年
11期
769-771
,共3页
李秀娟%黄胜起%王阿妮%郑辉%李时英%陈卫光%韦峰
李秀娟%黃勝起%王阿妮%鄭輝%李時英%陳衛光%韋峰
리수연%황성기%왕아니%정휘%리시영%진위광%위봉
脊柱炎,强直性%白细胞介素-17%核因子-κB受体活化因子配体%骨保护素
脊柱炎,彊直性%白細胞介素-17%覈因子-κB受體活化因子配體%骨保護素
척주염,강직성%백세포개소-17%핵인자-κB수체활화인자배체%골보호소
Spondylitis,ankylosing%Interleukin-17%Receptor activator of nuclear factor κB-ligand%Osteoprotegerin
目的 探讨白细胞介素(IL)-17、细胞核因子-κB受体活化因子配体(RANKL)及骨保护素在强直性脊柱炎(AS)发病机制中的作用及意义.方法 采用酶联免疫吸附试验(ELISA)检测44例AS患者及15名同期健康体检者血清IL-17、RANKL及骨保护素,流式细胞术检测人类白细胞抗原(HLA)-B27；统计学方法采用t检验、Spearman相关分析.结果 ①AS患者血清IL-17 [(45±12) pg/ml]、RANKL[(354±96) pg/ml]表达水平及RANKL/骨保护素[(6.5±3.4) pg/ml]比值均高于对照组(t=11.18,P＜0.05;t=18.66,P＜0.05；t=15.48,P＜0.05),并且中晚期血清RANKL表达水平高于早期血清RANKL水平(t=47.02,P＜0.05)；中晚期血清骨保护素表达水平显著高于早期,差异有统计学意义(t=15.48,P＜0.05).②AS组血清IL-17水平与RANKL呈正相关(r=0.532,P=0.021),血清IL-17、RANKL与Bath AS病情活动指数(BASDAI)均呈正相关(r=0.625,P=0.023；r=0.431,P=0.016).结论 IL-17与RANKL可能在AS发病中发挥作用.
目的 探討白細胞介素(IL)-17、細胞覈因子-κB受體活化因子配體(RANKL)及骨保護素在彊直性脊柱炎(AS)髮病機製中的作用及意義.方法 採用酶聯免疫吸附試驗(ELISA)檢測44例AS患者及15名同期健康體檢者血清IL-17、RANKL及骨保護素,流式細胞術檢測人類白細胞抗原(HLA)-B27；統計學方法採用t檢驗、Spearman相關分析.結果 ①AS患者血清IL-17 [(45±12) pg/ml]、RANKL[(354±96) pg/ml]錶達水平及RANKL/骨保護素[(6.5±3.4) pg/ml]比值均高于對照組(t=11.18,P＜0.05;t=18.66,P＜0.05；t=15.48,P＜0.05),併且中晚期血清RANKL錶達水平高于早期血清RANKL水平(t=47.02,P＜0.05)；中晚期血清骨保護素錶達水平顯著高于早期,差異有統計學意義(t=15.48,P＜0.05).②AS組血清IL-17水平與RANKL呈正相關(r=0.532,P=0.021),血清IL-17、RANKL與Bath AS病情活動指數(BASDAI)均呈正相關(r=0.625,P=0.023；r=0.431,P=0.016).結論 IL-17與RANKL可能在AS髮病中髮揮作用.
목적 탐토백세포개소(IL)-17、세포핵인자-κB수체활화인자배체(RANKL)급골보호소재강직성척주염(AS)발병궤제중적작용급의의.방법 채용매련면역흡부시험(ELISA)검측44례AS환자급15명동기건강체검자혈청IL-17、RANKL급골보호소,류식세포술검측인류백세포항원(HLA)-B27；통계학방법채용t검험、Spearman상관분석.결과 ①AS환자혈청IL-17 [(45±12) pg/ml]、RANKL[(354±96) pg/ml]표체수평급RANKL/골보호소[(6.5±3.4) pg/ml]비치균고우대조조(t=11.18,P＜0.05;t=18.66,P＜0.05；t=15.48,P＜0.05),병차중만기혈청RANKL표체수평고우조기혈청RANKL수평(t=47.02,P＜0.05)；중만기혈청골보호소표체수평현저고우조기,차이유통계학의의(t=15.48,P＜0.05).②AS조혈청IL-17수평여RANKL정정상관(r=0.532,P=0.021),혈청IL-17、RANKL여Bath AS병정활동지수(BASDAI)균정정상관(r=0.625,P=0.023；r=0.431,P=0.016).결론 IL-17여RANKL가능재AS발병중발휘작용.
Objective To investigate the role and value of interleukin (IL)-17,receptor activator of nuclear factors κB-ligand (RANKL),osteoprotegerin (OPG) in the pathogenesis of AS by detecting the expression of IL-17,RANKL,OPG.Methods IL-17,RANKL,OPG in 44 AS and 15 healthy controls were measured by ELISA.HLA-B27 was measured by flow cytometry.T-test,Spearman's correlation analysis were used for statisical analysis.Results ① Serum IL-17 [(45±12) pg/ml],RANKL [(354±96) pg/ml] levels and RANKL/OPG [(6.5±3.4) pg/ml] ratio were significantly higher in AS patients than those in normal controls (t=11.18,P＜0.05; t=18.66,P＜0.05; t=15.48,P＜0.05),and the serum RANKL levels was higher in AS patients in middle and late period than that in AS patients in early stage (t=47.02,P＜0.05).The serum OPG levels was higher in AS patients in middle and late period than that in AS patients in early stage (t=15.48,P＜0.05).② There were positive linea correletion,respectively,between the serum levels of IL-17 and RANKL (r=0.532,P=O.021),and between serum levels of IL-17 and disease activities of BASDAI (r=0.625,P=0.023),between serum levels of RAN KL and disease activities of BASDAI (r=0.431,P=0.016).Conclusion The surem levels of IL-17 and RANKL are increased in AS patient.
