中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2014年
8期
545-548,后插1
,共5页
关节炎,实验性%内皮抑素类%siRNA,小分子干扰%血管内皮生长因子类
關節炎,實驗性%內皮抑素類%siRNA,小分子榦擾%血管內皮生長因子類
관절염,실험성%내피억소류%siRNA,소분자간우%혈관내피생장인자류
Arthritis,experimental%Endostatins%RNA,small interfering%Vascular endothelial growth factors
目的 研究内皮抑素联合靶向血管内皮生长因子(VEGF)的siRNA对大鼠胶原诱导性关节炎(CIA)的治疗效果.方法 于大鼠足垫内注射2 mg/ml的牛Ⅱ型胶原进行免疫,复制CIA动物模型.免疫18d,用内皮抑素联合靶向VEGF的siRNA对动物模型进行治疗,32 d结束治疗.实验过程中通过一般情况观察、体质量、足踝体积等指标对动物模型进行评价,用ELISA法检测血浆中VEGF含量,用免疫组织化学技术检测关节滑膜组织内VEGF蛋白分布并计数,以及用踝关节病理组织切片来评价CIA病理改变情况.采用t检验进行统计分析.结果 内皮抑素治疗及内皮抑素联合siRNA治疗对CIA具有明显疗效.内皮抑素治疗组及内皮抑素联合siRNA治疗组VEGF蛋白含量分别为(15.7±0.3) ng/L和(14.7±0.5) ng/L,与模型组(17.5±0.3) ng/L相比,血清VEGF蛋白含量下降(P＜0.05).内皮抑素治疗组(153±27)及内皮抑素联合siRNA治疗组(126±71)的VEGF蛋白计数与模型组(903±82)比较,差异有统计学意义.结论 内皮抑素联合靶向VEGF的siRNA对大鼠CIA具有治疗效果.
目的 研究內皮抑素聯閤靶嚮血管內皮生長因子(VEGF)的siRNA對大鼠膠原誘導性關節炎(CIA)的治療效果.方法 于大鼠足墊內註射2 mg/ml的牛Ⅱ型膠原進行免疫,複製CIA動物模型.免疫18d,用內皮抑素聯閤靶嚮VEGF的siRNA對動物模型進行治療,32 d結束治療.實驗過程中通過一般情況觀察、體質量、足踝體積等指標對動物模型進行評價,用ELISA法檢測血漿中VEGF含量,用免疫組織化學技術檢測關節滑膜組織內VEGF蛋白分佈併計數,以及用踝關節病理組織切片來評價CIA病理改變情況.採用t檢驗進行統計分析.結果 內皮抑素治療及內皮抑素聯閤siRNA治療對CIA具有明顯療效.內皮抑素治療組及內皮抑素聯閤siRNA治療組VEGF蛋白含量分彆為(15.7±0.3) ng/L和(14.7±0.5) ng/L,與模型組(17.5±0.3) ng/L相比,血清VEGF蛋白含量下降(P＜0.05).內皮抑素治療組(153±27)及內皮抑素聯閤siRNA治療組(126±71)的VEGF蛋白計數與模型組(903±82)比較,差異有統計學意義.結論 內皮抑素聯閤靶嚮VEGF的siRNA對大鼠CIA具有治療效果.
목적 연구내피억소연합파향혈관내피생장인자(VEGF)적siRNA대대서효원유도성관절염(CIA)적치료효과.방법 우대서족점내주사2 mg/ml적우Ⅱ형효원진행면역,복제CIA동물모형.면역18d,용내피억소연합파향VEGF적siRNA대동물모형진행치료,32 d결속치료.실험과정중통과일반정황관찰、체질량、족과체적등지표대동물모형진행평개,용ELISA법검측혈장중VEGF함량,용면역조직화학기술검측관절활막조직내VEGF단백분포병계수,이급용과관절병리조직절편래평개CIA병리개변정황.채용t검험진행통계분석.결과 내피억소치료급내피억소연합siRNA치료대CIA구유명현료효.내피억소치료조급내피억소연합siRNA치료조VEGF단백함량분별위(15.7±0.3) ng/L화(14.7±0.5) ng/L,여모형조(17.5±0.3) ng/L상비,혈청VEGF단백함량하강(P＜0.05).내피억소치료조(153±27)급내피억소연합siRNA치료조(126±71)적VEGF단백계수여모형조(903±82)비교,차이유통계학의의.결론 내피억소연합파향VEGF적siRNA대대서CIA구유치료효과.
Objective To investigate the effect of treatment with endostatin combined with siRNA targeting VEGF on rats with collagen-induced arthritis (CIA).Methods Two mg/ml bovinetype Ⅱ collagen was injected into the rat footpad to build up the animal model of CIA.The experimental animal models were treated with endostatin combined with siRNA targeting VEGF 18 days later after immunization and the treatment ended 32 day later.The efficacy was evaluated by the weight,foot and ankle volume of rats.The levels of VEGF in plasma were detected by enzyme-linked immunosorbent assay (ELISA).The VEGF distribution within synovial tissue was detected and examined by immunohistochemical technique.The pathological changes of CIA were evaluated by the pathological changes of the biopsied ankle joints.Student's t test was used to evaluate the experimental data.Results The ELISA test showed that comparing with the model group (17.5±0.3),the endostatin group (15.7±0.3) ng/L and the endostatin combined siRNA targeting VEGF group (14.7±0.5) ng/L showed a significant efficacy in the treatment of CIA in rats (P＜0.05).The endostatin group (135±27) and the endostatin combined siRNA targeting VEGF group (126±71) were different in the number of VEGF in plasma and the VEGF distribution within synovial tissue (P＜0.05),the symptoms of arthritis in these rats were reduced than the model group.Conclusion Endostatin combined siRNA targeting VEGF has good therapeutic effect on rats with CIA.
