中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2014年
8期
552-554
,共3页
姚林虎%孟景红%李雪%魏平
姚林虎%孟景紅%李雪%魏平
요림호%맹경홍%리설%위평
脊柱炎,强直性%滤泡辅助性T细胞%白细胞介素21%CXCL13
脊柱炎,彊直性%濾泡輔助性T細胞%白細胞介素21%CXCL13
척주염,강직성%려포보조성T세포%백세포개소21%CXCL13
Spondylitis,ankylosing%Follicular helper T cells%Interleukin-21%CXCL13
目的 探讨AS患者外周血滤泡辅助性T细胞(Tfh)和细胞因子IL-21、趋化因子CXC配体13(CXCL13)的表达水平与AS临床指标的相关性及在AS发病机制中的作用.方法 流式细胞仪测定AS患者和健康对照外周血中Tfh的水平;ELISA法检测患者和健康对照血清中IL-21、CXCL13的表达水平;数据处理采用t检验、秩和检验、Spearman秩和相关分析.结果 AS患者外周血中Tfh的百分率(平均秩33.71)、IL-21[(299±27) ng/L]和CXCL13[(5.8±1.0) μg/L]表达率均明显高于健康对照组[平均秩23.54、(176±26) ng/L、(4.2±0.8)μg/L],差异均具有统计学意义(Z=-2.258,t=17.221,t=6.464,均P＜0.05);AS患者外周组与中轴组及HLA-B27阳性组与阴性组相比较,Tfh及细胞因子IL-21、CXCL13的表达水平差异均无统计学意义(P＞0.05);AS患者外周血中Tfh、IL-21、CXCL13的表达水平分别与ESR、CRP、BASDAI均无相关关系(P＞0.05);Tfh的水平分别与IL-21、CXCL13的表达水平无相关关系(P＞0.05).结论 AS患者外周血中Tfh及IL-21、CXCL13水平显著升高,提示Tfh及相关细胞因子可能在AS的发病过程中发挥着重要作用.
目的 探討AS患者外週血濾泡輔助性T細胞(Tfh)和細胞因子IL-21、趨化因子CXC配體13(CXCL13)的錶達水平與AS臨床指標的相關性及在AS髮病機製中的作用.方法 流式細胞儀測定AS患者和健康對照外週血中Tfh的水平;ELISA法檢測患者和健康對照血清中IL-21、CXCL13的錶達水平;數據處理採用t檢驗、秩和檢驗、Spearman秩和相關分析.結果 AS患者外週血中Tfh的百分率(平均秩33.71)、IL-21[(299±27) ng/L]和CXCL13[(5.8±1.0) μg/L]錶達率均明顯高于健康對照組[平均秩23.54、(176±26) ng/L、(4.2±0.8)μg/L],差異均具有統計學意義(Z=-2.258,t=17.221,t=6.464,均P＜0.05);AS患者外週組與中軸組及HLA-B27暘性組與陰性組相比較,Tfh及細胞因子IL-21、CXCL13的錶達水平差異均無統計學意義(P＞0.05);AS患者外週血中Tfh、IL-21、CXCL13的錶達水平分彆與ESR、CRP、BASDAI均無相關關繫(P＞0.05);Tfh的水平分彆與IL-21、CXCL13的錶達水平無相關關繫(P＞0.05).結論 AS患者外週血中Tfh及IL-21、CXCL13水平顯著升高,提示Tfh及相關細胞因子可能在AS的髮病過程中髮揮著重要作用.
목적 탐토AS환자외주혈려포보조성T세포(Tfh)화세포인자IL-21、추화인자CXC배체13(CXCL13)적표체수평여AS림상지표적상관성급재AS발병궤제중적작용.방법 류식세포의측정AS환자화건강대조외주혈중Tfh적수평;ELISA법검측환자화건강대조혈청중IL-21、CXCL13적표체수평;수거처리채용t검험、질화검험、Spearman질화상관분석.결과 AS환자외주혈중Tfh적백분솔(평균질33.71)、IL-21[(299±27) ng/L]화CXCL13[(5.8±1.0) μg/L]표체솔균명현고우건강대조조[평균질23.54、(176±26) ng/L、(4.2±0.8)μg/L],차이균구유통계학의의(Z=-2.258,t=17.221,t=6.464,균P＜0.05);AS환자외주조여중축조급HLA-B27양성조여음성조상비교,Tfh급세포인자IL-21、CXCL13적표체수평차이균무통계학의의(P＞0.05);AS환자외주혈중Tfh、IL-21、CXCL13적표체수평분별여ESR、CRP、BASDAI균무상관관계(P＞0.05);Tfh적수평분별여IL-21、CXCL13적표체수평무상관관계(P＞0.05).결론 AS환자외주혈중Tfh급IL-21、CXCL13수평현저승고,제시Tfh급상관세포인자가능재AS적발병과정중발휘착중요작용.
Objective To test the level of follicular helper T cells (Tfh) and interleukin (IL)-21,CXCL13 in the peripheral blood of patients with ankylosing spondylitis (AS),and to analyze the relationship between Tfh and clinic features and explore the possible immunological pathogenesis of AS.Methods The Tfh cells were obtained from patients and normal controls and detected by flow cytometry.While the levels of IL-21,CXCL13 in patients and normal controls were measured by enzyme-linked immunosorbent assay (ELISA) tests.Data analysis were performed by Student's t-test,Rank-sum test,Spearman's correlation test.Results The expression of CD4+CXCR5 qCOS + cells (Tfh) (mean rank 33.71) and IL-21 [(299±27) ng/L],CXCL13 [(5.8±1.0) μg/L] in the peripheral blood of AS was significantly higher than normal controls [mean rank 23.54,(176±26) ng/L,(4.2±0.8) μg/L] (Z=-2.258,t=17.221,t=6.464,all P＜0.05).It was similar in AS with peripheral joint involvement compared with AS of non-peripheral joint involvement,and there was no difference between AS patients with positive HLA-B27 and those without HLA-B27.Mean -while,no correlation was found between the expression of Tfh,IL-21,CXCL13 and level of ESR,CRP,BASDAI.And there was no significant correlation between the expression of Tfh and IL-21,CXCL13 (P＞0.05).Conclusion The expression of Tfh and the levels of IL-21,CXCL13 are increased significantly,but are not closely relatedto disease activity.These results indicate that the abnormality of Tfh may play an important role in the pathogenesis of AS.
