CAJ | 학술논문

目的研究PBC患者的骨髓间充质干细胞(BM-MSC)在生长与外分泌功能方面是否存在异常.方法取PBC患者及健康对照的新鲜骨髓,体外提取并扩增BM-MSC,以统计学t检验方法比较其在生长情况和分泌细胞因子情况方面的差异.结果 ①PBC患者BM-MSC的生长情况与对照组比较差异无统计学意义;②除TGF-β1在PBC患者BM-MSC上清平均水平低于对照组外[(2.6±1.9)与(8.2±6.7) ng/ml,t=-3.641,P=0.001],PBC患者与对照组的BM-MSC在细胞因子分泌功能方面基本一致;③对照组第5代以内的BM-MSC不同代次之间在细胞因子分泌方面功能基本相同,仅在IL-10[原代与第3代(18.5±5.0)与(12.4±3.1) pg/ml,t=2.368,P=0.045]、肝细胞生长因子[原代与第2代(0.21±0.07)与(0.35±0.08) ng/ml,t=-2.874,P=0.021]水平有小范围波动.结论 PBC患者的BM-MSC在生长情况及细胞因子分泌功能方面,除TGF-β1水平略低外,与对照比较差异无统计学意义.对照组MSC第2代细胞可能更适合治疗PBC.
목적 연구PBC환자적골수간충질간세포(BM-MSC)재생장여외분비공능방면시부존재이상.방법 취PBC환자급건강대조적신선골수,체외제취병확증BM-MSC,이통계학t검험방법비교기재생장정황화분비세포인자정황방면적차이.결과 ①PBC환자BM-MSC적생장정황여대조조비교차이무통계학의의;②제TGF-β1재PBC환자BM-MSC상청평균수평저우대조조외[(2.6±1.9)여(8.2±6.7) ng/ml,t=-3.641,P=0.001],PBC환자여대조조적BM-MSC재세포인자분비공능방면기본일치;③대조조제5대이내적BM-MSC불동대차지간재세포인자분비방면공능기본상동,부재IL-10[원대여제3대(18.5±5.0)여(12.4±3.1) pg/ml,t=2.368,P=0.045]、간세포생장인자[원대여제2대(0.21±0.07)여(0.35±0.08) ng/ml,t=-2.874,P=0.021]수평유소범위파동.결론 PBC환자적BM-MSC재생장정황급세포인자분비공능방면,제TGF-β1수평략저외,여대조비교차이무통계학의의.대조조MSC제2대세포가능경괄합치료PBC.
Objective To investigate the growth and exocrine function of BM-MSC derived from PBC patients.Methods To compare the growth patterns and cytokines secretions between PBC patients and healthy controls by student's t test.Results ① There was no difference in growth profile and speed between PBC patients and healthy controls.② The level of TGF-β1 was much lower in the supernatant of BM-MS from OBC patients than health controls [(2.6±1.9)vs (8.2±6.7)ng/ml,t=-3.641,P=0.001].There were no other differences between two groups' BM-MSC.③ The super natant concentration of interlukin-10 of the third BM-MSC subculture from healthy controls was lower than that of the primary subculture [(18.5±5.0) vs (12.4±3.1) pg/ml,t=2.368,P=0.045],and that of hepatic growth factor from the second subcuhure was higher than the primary subculture [(0.21±0.07) vs (0.35±0.08) ng/ml,t=-2.874,P=0.021].There were seldom discrepancies in other cytokines between different generations of BM-MSC.Conclusion BM-MSC from PBC patients may have almost the similar characters in growth pattern and cytokines secretion as,except the TGF-β1,which was much lower than those from healthy controls.The second subculture of BM-MSC might be more suitable for the treatment to patients with PBC.