CAJ | 학술논문

目的通过对成纤维细胞增殖机制的研究,探索RA中滑膜增生的机制.方法使用CCK-8法观察脂多糖对成纤维细胞增殖的刺激作用;通过共聚焦扫描显微镜观察成纤维细胞膜上Toll样受体(TLR)-4的表达情况;使用实时定量荧光PCR法检测巨噬细胞移动抑制因子(MIF)刺激成纤维细胞TLR-4表达量的增加情况,并经流式细胞分析技术进一步分析细胞膜上TLR-4表达量的变化.采用单因素方差分析和SNK-q检验进行统计学处理.结果脂多糖可以刺激成纤维细胞增殖;成纤维细胞固有(100％)表达TLR-4;经MIF刺激后成纤维细胞TLR-4的表达增加33％.结论 MIF可以通过上调TLR-4潜在性加强脂多糖诱导的成纤维细胞增殖.
목적 통과대성섬유세포증식궤제적연구,탐색RA중활막증생적궤제.방법 사용CCK-8법관찰지다당대성섬유세포증식적자격작용;통과공취초소묘현미경관찰성섬유세포막상Toll양수체(TLR)-4적표체정황;사용실시정량형광PCR법검측거서세포이동억제인자(MIF)자격성섬유세포TLR-4표체량적증가정황,병경류식세포분석기술진일보분석세포막상TLR-4표체량적변화.채용단인소방차분석화SNK-q검험진행통계학처리.결과 지다당가이자격성섬유세포증식;성섬유세포고유(100％)표체TLR-4;경MIF자격후성섬유세포TLR-4적표체증가33％.결론 MIF가이통과상조TLR-4잠재성가강지다당유도적성섬유세포증식.
Objective Based on the research of the mechanism of fibroblast proliferation,the principle of synovial hyperplasia is explored in rheumatoid arthritis (RA).Methods CCK-8 method was used to observe the stimulation effect of LPS on fibroblast proliferation.By confocal scanning microscopy,the expression of TLR-4 on fibroblast membrane was observed; Real-time fluorescent quantitative PCR method was used to detect the stimulatory effect of macrophage migration inhibitory factor on TLR-4 expression of fibrob-lasts and further analyzed the changes of TLR-4 expression on the cell membrane by flow cytometry.Results The LPS could stimulate fibroblast proliferation,and the fibroblasts expression of TLR4 per se.After stimulated by macrophage migration inhibitory factor,the expression of fibroblast TLR-4 was increased by about 20％-30％.Conclusion MIF increases the TLR-4 expression of fibroblasts that potentially enhance cell proliferation induced by LPS.