CAJ | 학술논문

目的建立大鼠Walker-256移植瘤模型,探索荷瘤大鼠吸入Carbogen气体(95％ O2+5％ CO2)后肿瘤R2*的信号变化情况及血氧水平依赖(BOLD)-MR技术应用于肿瘤乏氧检测的可能性.方法雌性SD大鼠95只,右下腹皮下接种Walker-256肿瘤细胞.成瘤SD大鼠肿瘤长至1.0～3.0 cm时行血氧水平依赖(BOLD)-MR检查.采用美国GE 3.0 T MR,动物表面线圈及多回波扰相稳态梯度回波(Multi-echo SPGR)序列,于大鼠自由吸入空气时和吸入Carbogen气体10 min后分别行BOLD-MR检查.扫描结束后,图像传至GE ADW 4.3工作站,用R2 Star分析软件进行图像后处理,计算肿瘤吸入空气时及吸入Carbogen气体10 min后R2*值(R2*a、R2*b),并求得其变化值△R2*=R2*b-R2*a,同时计算肿瘤的体积.吸入Carbogen气体前后R2*信号差异采用配对t检验,△R2*与R2*a之间及R2*a、△R2*与肿瘤体积大小之间行Pearson相关分析.结果接种95只SD大鼠,68只成瘤,成瘤率71.6％.肿瘤体积352～ 13 173 mm3,吸入空气及Carbogen气体后R2*值分别为(41.18±22.29)、(38.91±21.35)s-1,△R2 *值为(-2.26±3.90)s-1,差异有统计学意义(t=4.01,P＜0.01).△R2*值与R2*值呈负相关(r=-0.32,P＜0.05).肿瘤的△R2 *值与肿瘤的体积存在正相关性(r =0.35,P＜0.05),但是肿瘤的R2*a值与肿瘤体积之间没有明显相关性(r=-0.03,P＞0.05).结论 BOLD-MRI可以检测SD大鼠吸入Carbogen气体前后Walker-256肿瘤R2*信号值的变化.大鼠吸入Carbogen气体后Walker-256肿瘤的R2*值会发生明显下降,但不同荷瘤大鼠间差异较大.
목적 건립대서Walker-256이식류모형,탐색하류대서흡입Carbogen기체(95％ O2+5％ CO2)후종류R2*적신호변화정황급혈양수평의뢰(BOLD)-MR기술응용우종류핍양검측적가능성.방법 자성SD대서95지,우하복피하접충Walker-256종류세포.성류SD대서종류장지1.0～3.0 cm시행혈양수평의뢰(BOLD)-MR검사.채용미국GE 3.0 T MR,동물표면선권급다회파우상은태제도회파(Multi-echo SPGR)서렬,우대서자유흡입공기시화흡입Carbogen기체10 min후분별행BOLD-MR검사.소묘결속후,도상전지GE ADW 4.3공작참,용R2 Star분석연건진행도상후처리,계산종류흡입공기시급흡입Carbogen기체10 min후R2*치(R2*a、R2*b),병구득기변화치△R2*=R2*b-R2*a,동시계산종류적체적.흡입Carbogen기체전후R2*신호차이채용배대t검험,△R2*여R2*a지간급R2*a、△R2*여종류체적대소지간행Pearson상관분석.결과 접충95지SD대서,68지성류,성류솔71.6％.종류체적352～ 13 173 mm3,흡입공기급Carbogen기체후R2*치분별위(41.18±22.29)、(38.91±21.35)s-1,△R2 *치위(-2.26±3.90)s-1,차이유통계학의의(t=4.01,P＜0.01).△R2*치여R2*치정부상관(r=-0.32,P＜0.05).종류적△R2 *치여종류적체적존재정상관성(r =0.35,P＜0.05),단시종류적R2*a치여종류체적지간몰유명현상관성(r=-0.03,P＞0.05).결론 BOLD-MRI가이검측SD대서흡입Carbogen기체전후Walker-256종류R2*신호치적변화.대서흡입Carbogen기체후Walker-256종류적R2*치회발생명현하강,단불동하류대서간차이교대.
Objective To establish Walker-256 transplantation tumor model in SD Rats.To study of R2 * signal changes on murine Walker-256 tumor after inhaling Carbogen by blood oxygen level dependent (BOLD)-MR,and to explore the feasibility of BOLD-MRI on detecting tumor hypoxia.Methods Walker-256 tumor cell implanted subcutaneously in right lower abdomen of 95 female SD rats.MR was performed on the tumor-forming rats when the maximum diameter of tumor reached 1-3 cm,using a 3.0 T MR scanner equipped with a 3 inch animal surface coil BOLD-MRI was done by using a multiecho SPGR sequence during inhaling air and at 10 minute after inhaling Carbogen,respectively.All images were transferred to GE ADW 4.3 workstation,then a baseline R2* (R2 * a) and R2 * (R2 * b) after inhaling Carbogen of tumor was calculated using R2 Star analysis software and △R2 * was calculated through "△R2 * =R2 * b-R2 * a",meanwhile the volume of tumor were calculated as well.The difference of R2 * signal preand post-inhaling of Carbogen was compared with a paired t test,Pearson correlation was calculated between R2 * a,△R2 * and the volume of tumor,respectively.The correlation between △R2 * and R2 * a was also assessed by Pearson correlation.Results Sixty-eight of ninety-five female SD rats formed the tumor (71.6％).The volume of tumor was from 352 to 13 173 mm3.Mean △R2* decreased significantly (-2.26 ±3.90) s-1 from (41.18 ±22.29) s-1 during breathing air to(38.91 ±21.35) s-1 10 min after inhaling Carbogen (t =4.01,P ＜ 0.01).△R2 * was inversely correlated with R2 * (r =-0.32,P ＜ 0.05).The △R2 * was well correlated with volume of tumor (r =0.35,P ＜ 0.05),but R2 * a was not correlated with volume of tumor(r =-0.03,P ＞ 0.05).Conclusions BOLD-MRI can detect the R2 * signal change of murine Walker-256 tumor pre-and post-inhaling of Carbogen.The R2 * signal showed significant decrease after inhaling Carbogen,however,the individual variation was remarkable.