中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2013年
1期
10-13
,共4页
孙婷%丁大冬%李斌%陈桂林%韦永新%谢学顺%杨天权%吴庭枫%周幽心
孫婷%丁大鼕%李斌%陳桂林%韋永新%謝學順%楊天權%吳庭楓%週幽心
손정%정대동%리빈%진계림%위영신%사학순%양천권%오정풍%주유심
硼中子俘获疗法%二羟基苯丙氨酸硼%医院中子照射器%凋亡
硼中子俘穫療法%二羥基苯丙氨痠硼%醫院中子照射器%凋亡
붕중자부획요법%이간기분병안산붕%의원중자조사기%조망
Boron neutron capture therapy%Boronophenylalanine%In-hospital neutron irradiator%Apoptosis
目的 研究硼中子俘获疗法(BNCT)体外杀伤人黑色素瘤细胞的效应及机制.方法 首先检测黑色素瘤细胞A375吸收含硼化合物二羟基苯丙氨酸硼(BPA)的情况,然后采用医院中子照射器(IHNI-1)对含硼(10B)细胞进行照射.克隆存活实验检测细胞的放射敏感性,MTT法检测细胞增殖率,流式细胞术检测凋亡,Western blot检测胞质内细胞色素C表达和caspase-9的激活.结果 BPA孵育24 h,A375细胞10B浓度为(2.884±0.148)μg/107个细胞,达到了BNCT杀伤细胞的要求.富含10B的细胞经中子照射2.1 min后存活分数降低为对照组的58％(t=2.964,P＜0.05),细胞经中子照射后24 h增殖率下降为对照组的83％(t=3.286,P＜0.05),BNCT组细胞凋亡率达(55.2±7.9)％,明显高于对照组(t =9.754,P＜0.05),胞质内细胞色素C水平上升且caspase-9激活程度增加(t=7.625、8.307,P＜0.05).结论 BNCT能够杀伤黑色素瘤细胞,其机制可能通过线粒体途径诱导细胞凋亡.
目的 研究硼中子俘穫療法(BNCT)體外殺傷人黑色素瘤細胞的效應及機製.方法 首先檢測黑色素瘤細胞A375吸收含硼化閤物二羥基苯丙氨痠硼(BPA)的情況,然後採用醫院中子照射器(IHNI-1)對含硼(10B)細胞進行照射.剋隆存活實驗檢測細胞的放射敏感性,MTT法檢測細胞增殖率,流式細胞術檢測凋亡,Western blot檢測胞質內細胞色素C錶達和caspase-9的激活.結果 BPA孵育24 h,A375細胞10B濃度為(2.884±0.148)μg/107箇細胞,達到瞭BNCT殺傷細胞的要求.富含10B的細胞經中子照射2.1 min後存活分數降低為對照組的58％(t=2.964,P＜0.05),細胞經中子照射後24 h增殖率下降為對照組的83％(t=3.286,P＜0.05),BNCT組細胞凋亡率達(55.2±7.9)％,明顯高于對照組(t =9.754,P＜0.05),胞質內細胞色素C水平上升且caspase-9激活程度增加(t=7.625、8.307,P＜0.05).結論 BNCT能夠殺傷黑色素瘤細胞,其機製可能通過線粒體途徑誘導細胞凋亡.
목적 연구붕중자부획요법(BNCT)체외살상인흑색소류세포적효응급궤제.방법 수선검측흑색소류세포A375흡수함붕화합물이간기분병안산붕(BPA)적정황,연후채용의원중자조사기(IHNI-1)대함붕(10B)세포진행조사.극륭존활실험검측세포적방사민감성,MTT법검측세포증식솔,류식세포술검측조망,Western blot검측포질내세포색소C표체화caspase-9적격활.결과 BPA부육24 h,A375세포10B농도위(2.884±0.148)μg/107개세포,체도료BNCT살상세포적요구.부함10B적세포경중자조사2.1 min후존활분수강저위대조조적58％(t=2.964,P＜0.05),세포경중자조사후24 h증식솔하강위대조조적83％(t=3.286,P＜0.05),BNCT조세포조망솔체(55.2±7.9)％,명현고우대조조(t =9.754,P＜0.05),포질내세포색소C수평상승차caspase-9격활정도증가(t=7.625、8.307,P＜0.05).결론 BNCT능구살상흑색소류세포,기궤제가능통과선립체도경유도세포조망.
Objective To study the effect and underlying mechanism of boron neutron capture therapy (BNCT) on human melanoma cells.Methods The situation of boronophenylalanine (BPA) uptake of human melanoma cells A375 was detected and then the boron-10 (10B) enriched cells were irradiated by an in-hospital neutron irradiator (IHNI-1).The radiation sensitivity was measured using clonogenic survival assay,the proliferation was examined by MTT assay,apoptosis was determined using flow cytometry,and the protein expression of cytochrome C in cytosol and activation of caspase-9 was detected by Western blot.Results 10B concentration in A375 cells approached to (2.884 ± 0.148)μg/107 cells after 24 h culture with BPA,which met the requirement of BNCT.At 2.1 min after neutron radiation,the survival fraction of BNCT group was decreased to 58％ of control (t =2.964,P ＜ 0.05).At 24 h after BNCT,the cell viability was decreased to 83％ of control (t =3.286,P ＜ 0.05),the apoptosis ratio was (55.2 ± 7.9) ％ (t =9.754,P ＜ 0.05),and the protein level of cytochrome C in cytosol and the activativity of caspase-9 were also enhanced (t =7.625,8.307,P ＜ 0.05).Conclusions Human melanoma cells can be killed by BNCT due to apoptosis through a mitochondrial pathway.
