中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2013年
1期
14-18
,共5页
刘澜涛%刘建香%高刚%阮健磊%潘艳%朴春南%陈雪%苏旭
劉瀾濤%劉建香%高剛%阮健磊%潘豔%樸春南%陳雪%囌旭
류란도%류건향%고강%원건뢰%반염%박춘남%진설%소욱
电离辐射%调节性T细胞%表型
電離輻射%調節性T細胞%錶型
전리복사%조절성T세포%표형
Ionizing radiation%Regulatory T cell%Phenotype
目的 分析不同剂量照射对小鼠淋巴细胞及其调节性T细胞亚群数量和表型的影响.方法 用不同剂量60Coγ射线全身照射小鼠,分离胸腺和脾脏中的淋巴细胞,计数各样本细胞数后利用流式细胞仪分析CD4+T细胞和CD4+FOXP3+CD25+Treg细胞亚群的比例.结果 小鼠受照后胸腺细胞和脾脏细胞呈剂量依赖性减少,在照后4d降至最低(F=118.08、144.01,P＜0.05).较高剂量照射后,胸腺中Treg细胞数随时间逐渐减少,而脾脏中该细胞亚群逐渐恢复；较低剂量照射后,胸腺中Treg细胞数有所增加.与0 Gy组相比较,Treg细胞比例随剂量增加而增加(胸腺中F=5.16、89.44、3.01,P＜0.05;脾脏中F=52.02、32.13、27.45,P＜0.05).结论 不同剂量引起淋巴细胞及其Treg细胞亚群的辐射响应不同,其中Treg细胞亚群对较高剂量照射具有显著的辐射抗性,而低剂量照射可能诱导Treg细胞成熟分化或迁移.此外,淋巴细胞及其亚群的不同时间效应,也表明淋巴细胞成熟分化规律的差异.
目的 分析不同劑量照射對小鼠淋巴細胞及其調節性T細胞亞群數量和錶型的影響.方法 用不同劑量60Coγ射線全身照射小鼠,分離胸腺和脾髒中的淋巴細胞,計數各樣本細胞數後利用流式細胞儀分析CD4+T細胞和CD4+FOXP3+CD25+Treg細胞亞群的比例.結果 小鼠受照後胸腺細胞和脾髒細胞呈劑量依賴性減少,在照後4d降至最低(F=118.08、144.01,P＜0.05).較高劑量照射後,胸腺中Treg細胞數隨時間逐漸減少,而脾髒中該細胞亞群逐漸恢複；較低劑量照射後,胸腺中Treg細胞數有所增加.與0 Gy組相比較,Treg細胞比例隨劑量增加而增加(胸腺中F=5.16、89.44、3.01,P＜0.05;脾髒中F=52.02、32.13、27.45,P＜0.05).結論 不同劑量引起淋巴細胞及其Treg細胞亞群的輻射響應不同,其中Treg細胞亞群對較高劑量照射具有顯著的輻射抗性,而低劑量照射可能誘導Treg細胞成熟分化或遷移.此外,淋巴細胞及其亞群的不同時間效應,也錶明淋巴細胞成熟分化規律的差異.
목적 분석불동제량조사대소서림파세포급기조절성T세포아군수량화표형적영향.방법 용불동제량60Coγ사선전신조사소서,분리흉선화비장중적림파세포,계수각양본세포수후이용류식세포의분석CD4+T세포화CD4+FOXP3+CD25+Treg세포아군적비례.결과 소서수조후흉선세포화비장세포정제량의뢰성감소,재조후4d강지최저(F=118.08、144.01,P＜0.05).교고제량조사후,흉선중Treg세포수수시간축점감소,이비장중해세포아군축점회복；교저제량조사후,흉선중Treg세포수유소증가.여0 Gy조상비교,Treg세포비례수제량증가이증가(흉선중F=5.16、89.44、3.01,P＜0.05;비장중F=52.02、32.13、27.45,P＜0.05).결론 불동제량인기림파세포급기Treg세포아군적복사향응불동,기중Treg세포아군대교고제량조사구유현저적복사항성,이저제량조사가능유도Treg세포성숙분화혹천이.차외,림파세포급기아군적불동시간효응,야표명림파세포성숙분화규률적차이.
Objective To analyze the influence of ionizing radiation on the lymphocytes and its regulatory T cells in mice.Methods Mice were administered with whole body irradiation of γ-rays at different doses,and lymphocytes were separated from thymus and spleen,then the number of total cells were counted and the percentages of CD4 + T and CD4 + FOXP3 + CD25 + Treg lymphocytes were analyzed by using FACS.Results The lymphocyte numbers in thymus and spleen decreased in dosedependent manner and reached to the minimum at 4 d after irradiation (F =118.08,144.01,P ＜ 0.05).Exposure to higher dose(more than 1 Gy) decreased Treg number time-dependently in thymus,however increased it in spleen.On the contrary,exposure to lower dose (less than 0.75 Gy) increased Treg number in thymus.Besides,the percentage of Treg cells increased dose-dependently(in thymus,F =5.16,89.44,3.01,P ＜ 0.05 ; in spleen,F =52.02,32.13,27.45,P ＜ 0.05).Conclusions The radiation responses of lymphocytes and their Treg subpopulation vary with the different doses.Treg cells are resistant to high dose irradiation,however,their differentiation could be induced by low dose irradiation.In addition,the different time-dependent responses of lymphocytes and their subpopulation to ionizing radiation indicate the difference of lymphocyte maturation,differentiation and emigration.
