CAJ | 학술논문

目的观察不同剂量6MVX射线照射人结直肠癌细胞株HCT116后,体外细胞摄取18F-FLT的变化,分析18F-FLT用于早期预测肿瘤放射反应性的可行性.方法测定1.0×105 ～1.5×106个细胞、培养36、60、84 h条件下,结直肠癌细胞株HCT116的18F-FLT摄取率.测定细胞经0、2、4、6、8 Gy6 MVX射线照射后,不同时间点(24、48、72 h)18F-FLT的细胞摄取率及细胞摄取抑制率；同时测定不同剂量照射后的细胞生长曲线、细胞增殖及细胞周期情况.结果 HCT116细胞对18F-FLT摄取率达(18.97 ±1.16)％.18F-FLT细胞摄取抑制率在照射2、4、6、8 Gy后24 h分别为(32.10±0.02)％、(54.46±0.04)％、(62.74±0.04)％和(65.81±4.81)％；18F-FLT细胞摄取抑制率与照射剂量呈正相关. 18F-FLT细胞摄取率与细胞周期S期比例呈正相关.结论 18F-FLT细胞摄取率可以早期反映人结直肠癌HCT116细胞株对放射的反应性.
목적 관찰불동제량6MVX사선조사인결직장암세포주HCT116후,체외세포섭취18F-FLT적변화,분석18F-FLT용우조기예측종류방사반응성적가행성.방법 측정1.0×105 ～1.5×106개세포、배양36、60、84 h조건하,결직장암세포주HCT116적18F-FLT섭취솔.측정세포경0、2、4、6、8 Gy6 MVX사선조사후,불동시간점(24、48、72 h)18F-FLT적세포섭취솔급세포섭취억제솔；동시측정불동제량조사후적세포생장곡선、세포증식급세포주기정황.결과 HCT116세포대18F-FLT섭취솔체(18.97 ±1.16)％.18F-FLT세포섭취억제솔재조사2、4、6、8 Gy후24 h분별위(32.10±0.02)％、(54.46±0.04)％、(62.74±0.04)％화(65.81±4.81)％；18F-FLT세포섭취억제솔여조사제량정정상관. 18F-FLT세포섭취솔여세포주기S기비례정정상관.결론 18F-FLT세포섭취솔가이조기반영인결직장암HCT116세포주대방사적반응성.
Objective To evaluate the capability of 18 F-FLT uptake and investigate the early radiation response of human colorectal cancer cells HCT116 exposed to 6 MV X-rays.Methods 3.7 kBq 18F-FLT was added to HCT116 cells with different cell numbers (1.0 × 105-1.5 × 106) and cultured with different times (36,60,84 h).The 18F-FLT uptake rate was measured with a γ-counter after exposed to different does of 6 MV X-rays (0,2,4,6,8 Gy) after 24,48,and 72 h of irradiation.Then the cell uptake inhibition rate,cell proliferation,and cell cycle phase were measured.Results The uptake rate of 18F-FLT in HCT116 was (18.97 ± 1.16)％.The 18F-FLT uptake inhibition rates at 24 h after different does of irradiation (2,4,6,8 Gy) were (32.10±0.02)％,(54.46 ±0.04)％,(62.74 ±0.04)％,and (65.81 ±4.81)％,respectively,which was positively correlated with radiation dose.Conclusions The 18F-FLT uptake rate of human colorectal cancer HCT116 cells could be used to evaluate the early radiation response.