CAJ | 학술논문

目的比较18F-氟代脱氧胸腺嘧啶(18F-FLT)和18F-氟代脱氧葡萄糖(18F-FDG)在反映食管癌细胞受照后超早期生物学反应的差异.方法将人食管癌Eca-109细胞分别接受5、10、15 Gy剂量X射线照射,照射后2、4、8h检测细胞对18F-FDG和18F-FLT摄取率的变化,以及细胞相对存活率和ATP表达情况的变化.结果 5 Gy照射后2、4h,细胞对18F-FDG摄取率与对照组相比分别减少了9.45％和16.4％,差异无统计学意义；但15 Gy照后2h,对18F-FDG摄取率却增加了26.5％(=3.04,P＜0.05),其余照射组对18F-FDG摄取率均有明显减少(F=25.75,P＜0.05).5 Gy照后2h,18F-FLT摄取率(3.65±0.41)％与对照组(4.00±0.42)％相比,差异无统计学意义,其余照射组对18F-FLT摄取率与对照组比较均有明显减少(F=33.93,P＜0.05).在5、10、15 Gy照后2、4、8h,各组细胞相对存活率差异无统计学意义.经不同剂量照射后,细胞对18F-FLT摄取率与ATP浓度之间的相关性(r=0.887,P＜0.05)优于18F-FDG与ATP浓度之间的相关性(r=0.622,P＞0.05).结论 18F-FDG和18 F-FLT两者均可反映食管癌细胞照射后超早期生物学反应,18F-FLT比18F-FDG能较好地反映食管癌细胞照射后超早期生物学反应.
목적 비교18F-불대탈양흉선밀정(18F-FLT)화18F-불대탈양포도당(18F-FDG)재반영식관암세포수조후초조기생물학반응적차이.방법 장인식관암Eca-109세포분별접수5、10、15 Gy제량X사선조사,조사후2、4、8h검측세포대18F-FDG화18F-FLT섭취솔적변화,이급세포상대존활솔화ATP표체정황적변화.결과 5 Gy조사후2、4h,세포대18F-FDG섭취솔여대조조상비분별감소료9.45％화16.4％,차이무통계학의의；단15 Gy조후2h,대18F-FDG섭취솔각증가료26.5％(=3.04,P＜0.05),기여조사조대18F-FDG섭취솔균유명현감소(F=25.75,P＜0.05).5 Gy조후2h,18F-FLT섭취솔(3.65±0.41)％여대조조(4.00±0.42)％상비,차이무통계학의의,기여조사조대18F-FLT섭취솔여대조조비교균유명현감소(F=33.93,P＜0.05).재5、10、15 Gy조후2、4、8h,각조세포상대존활솔차이무통계학의의.경불동제량조사후,세포대18F-FLT섭취솔여ATP농도지간적상관성(r=0.887,P＜0.05)우우18F-FDG여ATP농도지간적상관성(r=0.622,P＞0.05).결론 18F-FDG화18 F-FLT량자균가반영식관암세포조사후초조기생물학반응,18F-FLT비18F-FDG능교호지반영식관암세포조사후초조기생물학반응.
Objective To compare the difference of 3'-deoxy-3'-18F-fluorothymidine (18F-FLT)and 2'-deoxy-2'-18F-fluoro-D-glucose (18 F-FDG) in the ultra-early biological response of esophageal carcinoma cells after irradiation.Methods After 2,4 and 8 h of irradiation with different doses (5,10,15 Gy),the uptake ratio of 18F-FDG and 18F-FLT,survival,and ATP level of esophageal carcinoma cells were detected.Results After 2 and 4 h of 5 Gy irradiation,the cellular 18F-FDG uptake ratio was decreased by 9.45％ and 16.4％.After 2 h of 15 Gy irradiation,this uptake ratio was increased by 26.5％ (t =3.04,P ＜ 0.05).But the 18F-FDG uptake ratios of the other experimental groups were decreased remarkably (F =25.75,P ＜0.05).On the other side,after 2 h of 5 Gy irradiation,the 18F-FLT uptake ratio had no statistically difference [(3.65 ±0.17)％ vs (4.00 ±0.17)％,P ＞0.05],and 18F-FLT uptake ratios of the other groups were decreased notably (F =33.93,P ＜ 0.05).The relative survival of esophageal carcinoma cells after 2,4 and 8 h of irradiation with different doses (5,10,15 Gy) has no significant difference.Line regression analysis indicated a significant correlation between 18F-FLT and ATP levels (r =0.887,P ＜ 0.05),whereas a less correlation was found between 18 F-FDG and ATP levels (r =0.622,P ＞ 0.05).Conclusions Both 18F-FDG and 18F-FLT can evaluate the ultra-early irradiation response of esophageal carcinoma cells,but 18F-FLT may be more specific than 18F-FDG in monitoring the ultra-early irradiation response.