中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2013年
2期
135-137
,共3页
孙宝胜%左雅芳%田琦%欧健%张国胜%刘江山%刘博宇%刘士新
孫寶勝%左雅芳%田琦%歐健%張國勝%劉江山%劉博宇%劉士新
손보성%좌아방%전기%구건%장국성%류강산%류박우%류사신
电离辐射%胸腺淋巴瘤%BCL11b%miR-92a
電離輻射%胸腺淋巴瘤%BCL11b%miR-92a
전리복사%흉선림파류%BCL11b%miR-92a
Ionizing radiation%Thymic lymphoma%BCL11b%miR-92a
目的 采用电离辐射诱导的小鼠胸腺淋巴瘤动物模型,检测抑癌基因BCL11b和miR-92a的表达变化,探讨miR-92a对BCL11b基因的调控作用.方法 BALB/c小鼠采用γ射线全身照射,单次照射剂量和剂量率分别为1.75 Gy和0.382 Gy/min,每周1次,连续照射4周,计算电离辐射诱导小鼠胸腺淋巴瘤发生率.采用实时定量PCR法检测miR-92a表达变化情况,Westernblot方法检测BCL11b蛋白表达变化情况；将miR-92a序列插入载体pcDNA-DEST-47中构建真核表达质粒；构建BCL11b基因3'UTR-荧光素酶报告质粒,与pcDNA-DEST-miR-92a质粒共转染至EL-4细胞,分析miR-92a对BCL11b表达的调控作用.结果 辐射诱导的BALB/c小鼠胸腺淋巴瘤的发生率为58.51％.胸腺淋巴瘤细胞中BCL11b表达下调,而miR-92a表达上调,两者之间存在显著的负相关(r=-0.827,P＜0.05).psiCHECK 2-BCL11b和pcDNA-DEST-miR-92a两种质粒共转染组的荧光素酶表达明显低于单独转染的空载体和靶基因BCL11b组(t=3.42,P＜0.05).结论 电离辐射诱导的小鼠胸腺淋巴瘤中miR-92a与BCL11b表达的负相关,提示miR-92a可能参与了辐射诱发肿瘤中BCL11b蛋白表达的调控作用.
目的 採用電離輻射誘導的小鼠胸腺淋巴瘤動物模型,檢測抑癌基因BCL11b和miR-92a的錶達變化,探討miR-92a對BCL11b基因的調控作用.方法 BALB/c小鼠採用γ射線全身照射,單次照射劑量和劑量率分彆為1.75 Gy和0.382 Gy/min,每週1次,連續照射4週,計算電離輻射誘導小鼠胸腺淋巴瘤髮生率.採用實時定量PCR法檢測miR-92a錶達變化情況,Westernblot方法檢測BCL11b蛋白錶達變化情況；將miR-92a序列插入載體pcDNA-DEST-47中構建真覈錶達質粒；構建BCL11b基因3'UTR-熒光素酶報告質粒,與pcDNA-DEST-miR-92a質粒共轉染至EL-4細胞,分析miR-92a對BCL11b錶達的調控作用.結果 輻射誘導的BALB/c小鼠胸腺淋巴瘤的髮生率為58.51％.胸腺淋巴瘤細胞中BCL11b錶達下調,而miR-92a錶達上調,兩者之間存在顯著的負相關(r=-0.827,P＜0.05).psiCHECK 2-BCL11b和pcDNA-DEST-miR-92a兩種質粒共轉染組的熒光素酶錶達明顯低于單獨轉染的空載體和靶基因BCL11b組(t=3.42,P＜0.05).結論 電離輻射誘導的小鼠胸腺淋巴瘤中miR-92a與BCL11b錶達的負相關,提示miR-92a可能參與瞭輻射誘髮腫瘤中BCL11b蛋白錶達的調控作用.
목적 채용전리복사유도적소서흉선림파류동물모형,검측억암기인BCL11b화miR-92a적표체변화,탐토miR-92a대BCL11b기인적조공작용.방법 BALB/c소서채용γ사선전신조사,단차조사제량화제량솔분별위1.75 Gy화0.382 Gy/min,매주1차,련속조사4주,계산전리복사유도소서흉선림파류발생솔.채용실시정량PCR법검측miR-92a표체변화정황,Westernblot방법검측BCL11b단백표체변화정황；장miR-92a서렬삽입재체pcDNA-DEST-47중구건진핵표체질립；구건BCL11b기인3'UTR-형광소매보고질립,여pcDNA-DEST-miR-92a질립공전염지EL-4세포,분석miR-92a대BCL11b표체적조공작용.결과 복사유도적BALB/c소서흉선림파류적발생솔위58.51％.흉선림파류세포중BCL11b표체하조,이miR-92a표체상조,량자지간존재현저적부상관(r=-0.827,P＜0.05).psiCHECK 2-BCL11b화pcDNA-DEST-miR-92a량충질립공전염조적형광소매표체명현저우단독전염적공재체화파기인BCL11b조(t=3.42,P＜0.05).결론 전리복사유도적소서흉선림파류중miR-92a여BCL11b표체적부상관,제시miR-92a가능삼여료복사유발종류중BCL11b단백표체적조공작용.
Objective To compare the differences of the position, volume and matching index (MI) between internal gross tumor volumes ( IGTVs) determined by four methods based on four dimensional CT (4DCT) and three dimensional CT (3DCT) scans during free reathing and IGTV determined hy one method based on 3DCT ssisted with active breathing control. Methods Eighteen patients with peripheral lung cancer underwent 3DCT and 4DCT simulation scans during free hreathing and then underwent 3DCT simulation scans in end inspiration hold ( CTFIH )and end expiration hold ( CTEFH ).IGTVs were acquired using five methods: the gross tumor volme ( GTV) contours from ten respiratory phases were combined into IGTV10; The GTV contours from 0％ and 50％ phases were combined into ICTV2; IGTVMIP was the GTV contour delineated from the maximum intensity projection ( MIP) ; IGTV3D was acquired from the enlargement of 3DCT-based GTV by each spatial direction on the motion amplitude measured in 4DCT; IGTVABC was acquired by combining GTVs from CTEIR and CTEEH. Results In upper
lobe group, there were significant differences in centroid position between ICTVABC and IGTV10 in lateral ( LR) , and IGTVMIP in anterio-posterior ( AP) directions respectively (t =- 2. 377,-2. 199, P ＜0. 05) ,and there were significant differences in centroid position between IGTVABC and IGTV2 , IGTV3D in both LR and AP directions (t = - 2. 185 - - 2. 767, P ＜ 0. 05). In lower lobe group, there were significant
differences in centroid position between IGTVABC and ICTV10, IGTVMIP, IGTV3D in LR direction (t =3. 950 -4. 543 , P＜0. 05). ,Either in the upper lobe group or in the lower lobe group, IGTVABC was smaller than ICTV10 or IGTV3D significantly (Z = - 3. 180 - - 2. 023 , P＜ 0. 05) , and the Mls between ICTVABC
and otber IGTVs were larger in the upper lobe group thanin the lower lobe group significantly (Z = -2. 419 -2. 119, P＜0. 05 ) . Conclrrsions IGTVABC is not completely coincide with IGTVs acquired based on 4DCT or 3DCT during free breathing, and the spatial mismatch correlated with the tumor position,the spatial mismatch is more significant for middle-lower lobe tumor than upper lobe tumor. IGTVABC are larger than IGTV2 but smaller than other IGTVs acquired based on 4DCT and IGTV acquired based on 3DCT during free breathing.
