CAJ | 학술논문

目的通过观察不同剂量60Co γ射线照射后小鼠外周血网织红细胞微核率的变化,为使外周血网织红细胞微核成为探索快速高通量的生物剂量计提供科学依据.方法 60 Co γ射线照射ICR小鼠,按不同照射剂量分为0、0.5、1、2、4和8 Gy组,眼球取血,流式细胞术(FCM)检测和显微镜观察小鼠外周血网织红细胞微核率的变化.结果流式细胞术检测网织红细胞微核率随剂量增加逐渐增加,2 Gy达峰值,之后随剂量的增加而减少；显微镜观察的网织红细胞微核率变化趋势与流式细胞术检测结果基本一致.照射剂量在0～2 Gy剂量范围内,小鼠的外周血网织红细胞微核率的剂量-效应关系满足直线模型(R2=0.9063),并且2 Gy照射组与0 Gy组相比差异有统计学意义(t=-2.856,P＜0.05).结论流式细胞术检测外周血网织红细胞微核率,在一定剂量范围内可成为早期快速、高通量的辐射损伤生物剂量计.
목적 통과관찰불동제량60Co γ사선조사후소서외주혈망직홍세포미핵솔적변화,위사외주혈망직홍세포미핵성위탐색쾌속고통량적생물제량계제공과학의거.방법 60 Co γ사선조사ICR소서,안불동조사제량분위0、0.5、1、2、4화8 Gy조,안구취혈,류식세포술(FCM)검측화현미경관찰소서외주혈망직홍세포미핵솔적변화.결과 류식세포술검측망직홍세포미핵솔수제량증가축점증가,2 Gy체봉치,지후수제량적증가이감소；현미경관찰적망직홍세포미핵솔변화추세여류식세포술검측결과기본일치.조사제량재0～2 Gy제량범위내,소서적외주혈망직홍세포미핵솔적제량-효응관계만족직선모형(R2=0.9063),병차2 Gy조사조여0 Gy조상비차이유통계학의의(t=-2.856,P＜0.05).결론 류식세포술검측외주혈망직홍세포미핵솔,재일정제량범위내가성위조기쾌속、고통량적복사손상생물제량계.
Objective To detect the changes of the percentage of micronucleated reticulocytes (MN-RET) in the peripheral blood of mice exposed to 60Co γ-rays,in order to provide evidence for a new biomarker of radiation biodosimetry.Methods ICR mice were irradiated in whole body with 0,0.5,1,2,4 and 8 Gy at a dose rate of 0.24 Gy/min.Peripheral blood was collected for MN-RET assay using a flow cytometry.Results The percentage of peripheral MN-RET increased steadily with irradiation doses up to 2 Gy and then had a downtrend beyond 2 Gy.The changes of MN-RET observed with a microscope were consistent with the results from flow cytometry.The dose response of the MN-RET fitted to a lineal model (R2 =0.9063),and the MN-RET at 2 Gy was significantly higher than that of nonirradiated control (t =-2.856,P ＜ 0.05).Conclusion Percentage of peripheral M N-RET could be an early,rapid and high-throughput radiation bio-dosimeter in certain range of doses.