中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2013年
3期
252-255
,共4页
杜丽%孙嵘%马琼%李志慧%蔡金玲%杨晓云%满其航%崔玉芳
杜麗%孫嶸%馬瓊%李誌慧%蔡金玲%楊曉雲%滿其航%崔玉芳
두려%손영%마경%리지혜%채금령%양효운%만기항%최옥방
毫米波%应激%细胞因子%下丘脑-垂体-肾上腺皮质轴
毫米波%應激%細胞因子%下丘腦-垂體-腎上腺皮質軸
호미파%응격%세포인자%하구뇌-수체-신상선피질축
Millimeter-wave%Stress%Cytokine%Hypothalamo-pituitary-adrenal axis
目的 观察35 GHz毫米波诱发的急性应激反应中重要细胞因子及下丘脑-垂体-肾上腺皮质(HPA)轴相关激素的表达规律,旨在探索神经·内分泌·免疫系统之间内在的关联.方法 以420 mW/cm2功率密度的35 GHz毫米波源急性辐照BALB/c小鼠60 s.于照后不同时间,用ELISA方法检测血清中IFN-γ、IL-4等细胞因子的表达水平,并用RT-PCR和免疫组织化学方法检测下丘脑促肾上腺皮质素释放激素(CRH)和海马区糖皮质激素受体(GR)的变化.结果 Th1和Th2型细胞因子IFN-γ和IL-4在照后3d均升高至峰值(t=-6.59、-2.28,P＜0.05),至照后7d仍明显高于对照组；其比值在照后1和3d明显增高,即发生了明显的Th1/Th2平衡向Th1免疫反应漂移的现象.毫米波急性辐照后下丘脑CRH mRNA含量出现持续性升高,至7d升高至峰值(t=-7.03,P ＜0.05)；而海马GR mRNA含量在3d达到峰值后迅速降低至对照组水平.下丘脑CRH和海马GR蛋白表达的趋势与其mRNA水平变化基本一致.结论 小鼠经35 GHz毫米波急性辐照后,Th1/Th2平衡向Th1方向偏移；同时应激增加的下丘脑CRH和海马GR对IFN-γ等因子的反馈抑制,使向Th1偏移的免疫失衡现象得以改善,可缓解过度应激造成的免疫损伤.
目的 觀察35 GHz毫米波誘髮的急性應激反應中重要細胞因子及下丘腦-垂體-腎上腺皮質(HPA)軸相關激素的錶達規律,旨在探索神經·內分泌·免疫繫統之間內在的關聯.方法 以420 mW/cm2功率密度的35 GHz毫米波源急性輻照BALB/c小鼠60 s.于照後不同時間,用ELISA方法檢測血清中IFN-γ、IL-4等細胞因子的錶達水平,併用RT-PCR和免疫組織化學方法檢測下丘腦促腎上腺皮質素釋放激素(CRH)和海馬區糖皮質激素受體(GR)的變化.結果 Th1和Th2型細胞因子IFN-γ和IL-4在照後3d均升高至峰值(t=-6.59、-2.28,P＜0.05),至照後7d仍明顯高于對照組；其比值在照後1和3d明顯增高,即髮生瞭明顯的Th1/Th2平衡嚮Th1免疫反應漂移的現象.毫米波急性輻照後下丘腦CRH mRNA含量齣現持續性升高,至7d升高至峰值(t=-7.03,P ＜0.05)；而海馬GR mRNA含量在3d達到峰值後迅速降低至對照組水平.下丘腦CRH和海馬GR蛋白錶達的趨勢與其mRNA水平變化基本一緻.結論 小鼠經35 GHz毫米波急性輻照後,Th1/Th2平衡嚮Th1方嚮偏移；同時應激增加的下丘腦CRH和海馬GR對IFN-γ等因子的反饋抑製,使嚮Th1偏移的免疫失衡現象得以改善,可緩解過度應激造成的免疫損傷.
목적 관찰35 GHz호미파유발적급성응격반응중중요세포인자급하구뇌-수체-신상선피질(HPA)축상관격소적표체규률,지재탐색신경·내분비·면역계통지간내재적관련.방법 이420 mW/cm2공솔밀도적35 GHz호미파원급성복조BALB/c소서60 s.우조후불동시간,용ELISA방법검측혈청중IFN-γ、IL-4등세포인자적표체수평,병용RT-PCR화면역조직화학방법검측하구뇌촉신상선피질소석방격소(CRH)화해마구당피질격소수체(GR)적변화.결과 Th1화Th2형세포인자IFN-γ화IL-4재조후3d균승고지봉치(t=-6.59、-2.28,P＜0.05),지조후7d잉명현고우대조조；기비치재조후1화3d명현증고,즉발생료명현적Th1/Th2평형향Th1면역반응표이적현상.호미파급성복조후하구뇌CRH mRNA함량출현지속성승고,지7d승고지봉치(t=-7.03,P ＜0.05)；이해마GR mRNA함량재3d체도봉치후신속강저지대조조수평.하구뇌CRH화해마GR단백표체적추세여기mRNA수평변화기본일치.결론 소서경35 GHz호미파급성복조후,Th1/Th2평형향Th1방향편이；동시응격증가적하구뇌CRH화해마GR대IFN-γ등인자적반궤억제,사향Th1편이적면역실형현상득이개선,가완해과도응격조성적면역손상.
Objective To investigate the expression pattern of cytokines and HPA axis hormones in acute stress reaction induced by 35 GHz millimeter-wave (MMW) radiation,and to explore the intrinsic association among the nerve-endocrine-immune system.Methods BALB/c mice were exposed to 35 GHz MMW with an average power density of 420 mW/cm2 for 60 s.The contents of IFN-γ and IL-4 in serum were measured and the expression level of hypothalamic corticotropin releasing hormone (CRH) and hippocampus glucocorticoid receptor (GR) in brain were analyzed at different time points after MMW exposure.Results The levels of IFN-γ and IL-4 and the cytokines of Thl and Th2 increased to peak levels (t =-6.59,-2.28,P ＜ 0.05) at 3 d after exposure,and were still high up to 7 d after exposure.The ratio of Th1/Th2 (IFN-γ/IL-4) increased significantly at 1 d and 3 d post-exposure,which suggested that Th1/Th2 balance drifted to Th1 immune response.After acute MMW exposure,the level of hypothalamic CRH mRNA increased gradually and approached to a peak value at 7 d (t =-7.03,P ＜0.05).The expression level of hippocampal GR mRNA reached the peak at 3 d and then declined to control level.Meanwhile,the changes of hypothalamic CRH and hippocampal GR were consistent with the changes of their mRNA expressions.Conclusions Th1/Th2 balance in mice shifts toward Th1 after 35 GHz MMW acute exposure and the imbalance of Th1 shift can be improved obviously due to the feedback inhibition of IFN-γ and other cytokines by increased hypothalamic CRH and hippocampal GR induced by stress,which can alleviate the immune damage caused by excessive stress.
