CAJ | 학술논문

目的探讨甘氨双唑钠修饰纳米金制备及放射增敏效果.方法把甘氨双唑钠修饰到已连接聚乙二醇的纳米金上,纳米金粒径18 nm.利用扫描电镜观察肺腺癌(A549)细胞吞噬甘氨双唑钠-纳米金的现象.将培养的肺腺癌细胞分为甘氨双唑钠组、纳米金组、甘氨双唑钠·纳米金组、对照组(不加药组).用四甲基偶氮唑盐比色法和克隆形成实验对肺腺癌(A549)细胞进行放射增敏的研究.结果甘氨双唑钠-纳米金能进入细胞质和细胞核；浓度为0.003 mg/ml纳米金和甘氨双唑钠-纳米金没有明显的细胞毒性；甘氨双唑钠-纳米金组相对于甘氨双唑钠组、纳米金组、对照组,D0、Dq出现了下调；接受2、4、6和8 Gy剂量照射后,甘氨双唑钠-纳米金组肺腺癌细胞的存活率与其他3组相比,均明显下降(F=4.8、14.5、5.7、7.6,P＜0.05).结论甘氨双唑钠修饰的纳米金能增加肺腺癌细胞的辐射敏感性.
목적 탐토감안쌍서납수식납미금제비급방사증민효과.방법 파감안쌍서납수식도이련접취을이순적납미금상,납미금립경18 nm.이용소묘전경관찰폐선암(A549)세포탄서감안쌍서납-납미금적현상.장배양적폐선암세포분위감안쌍서납조、납미금조、감안쌍서납·납미금조、대조조(불가약조).용사갑기우담서염비색법화극륭형성실험대폐선암(A549)세포진행방사증민적연구.결과 감안쌍서납-납미금능진입세포질화세포핵；농도위0.003 mg/ml납미금화감안쌍서납-납미금몰유명현적세포독성；감안쌍서납-납미금조상대우감안쌍서납조、납미금조、대조조,D0、Dq출현료하조；접수2、4、6화8 Gy제량조사후,감안쌍서납-납미금조폐선암세포적존활솔여기타3조상비,균명현하강(F=4.8、14.5、5.7、7.6,P＜0.05).결론 감안쌍서납수식적납미금능증가폐선암세포적복사민감성.
Objective To study the radioseusitization effect of gold nanoparticles modified by sodium glycididazole.Methods The sodium glycididazole was connected to gold nanoparticle,in dimension of about 18 nm,that had been modified with polyethylene glycol.The nanoparticle-swallowing efficiency of lung adenocarcinoma A549 cells was observed by a scanning electron microscope.Cells were divided into four groups:sodium glycididazole group,gold nanoparticles group,sodium glycididazole-gold nanoparticles group,and no drug control group.The radiosensitivity was detected by MTT and colony formation assays.Results Sodium glycididazole-gold nanoparticles could enter the cell cytoplasm and nucleus.The concentration of 0.003 mg/ml gold nanoparticles and sodium glycididazole-gold nanoparticles had no obvious cytotoxic effect.After irradiation of 2,4,6,8 Gy,the cell survival of the sodium glycididazole-gold nanoparticle group was significantly lower than that of the other three groups (F =4.8,14.5,5.7,7.6,P ＜0.05) and the D0 and Dq values of the sodium glycididazole-gold nanoparticle group were significantly lower than those of other three groups.Conclusion Sodium glycididazole-gold nanoparticles can enhance the radiosensitivity of lung adenocarcinoma cells.