中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2013年
3期
278-281
,共4页
张丽丽%刘希光%赵如森%宋浩%张红军%王玉坤%李敬东%李伟%梁晔
張麗麗%劉希光%趙如森%宋浩%張紅軍%王玉坤%李敬東%李偉%樑曄
장려려%류희광%조여삼%송호%장홍군%왕옥곤%리경동%리위%량엽
凋亡素2配体%食管癌细胞%放射敏感性%凋亡%G2/M期阻滞
凋亡素2配體%食管癌細胞%放射敏感性%凋亡%G2/M期阻滯
조망소2배체%식관암세포%방사민감성%조망%G2/M기조체
TRAIL%Esophageal cancer cell%Radiosensitivity%Apoptosis%G2/M Arrest
目的 研究凋亡素2配体(Apo-2 ligand,Apo2L),又称肿瘤坏死因子相关凋亡诱导配体(tumor necrosis factor-related apoptosis inducing ligand,TRAIL)对体外培养食管癌细胞株Eca-109放射敏感性的影响.方法 MTT法检测TRAIL对Eca-109细胞的毒性；克隆形成实验检测TRAIL对Eca-109细胞的放射增敏作用；流式细胞仪(FCM)技术分析TRAIL对凋亡率及细胞周期的影响.结果 100～800 μg/ml TRAIL随浓度升高对Eca-109细胞的增殖抑制率增大,药物浓度与细胞抑制率正相关(r=0.981,P＜0.01),50％抑制浓度(IC50)为637 μg/ml;200 μg/ml TRAIL能增加Eca-109细胞的放射敏感性,放射增敏比为1.219；不同剂量(0～8 Gy)照射后,给药组的凋亡率均高于单照射组(F=16.97、76.65、92.86、209.66,P＜0.05),给药组G2/M期细胞比例较单照射组增加(F=9.40、84.99、87.61、2025.85,P＜0.05).结论 TRAIL可以抑制Eca-109细胞的生长,诱导细胞凋亡和G2/M期阻滞,后者可能与TRAIL的放射增敏机制有关.
目的 研究凋亡素2配體(Apo-2 ligand,Apo2L),又稱腫瘤壞死因子相關凋亡誘導配體(tumor necrosis factor-related apoptosis inducing ligand,TRAIL)對體外培養食管癌細胞株Eca-109放射敏感性的影響.方法 MTT法檢測TRAIL對Eca-109細胞的毒性；剋隆形成實驗檢測TRAIL對Eca-109細胞的放射增敏作用；流式細胞儀(FCM)技術分析TRAIL對凋亡率及細胞週期的影響.結果 100～800 μg/ml TRAIL隨濃度升高對Eca-109細胞的增殖抑製率增大,藥物濃度與細胞抑製率正相關(r=0.981,P＜0.01),50％抑製濃度(IC50)為637 μg/ml;200 μg/ml TRAIL能增加Eca-109細胞的放射敏感性,放射增敏比為1.219；不同劑量(0～8 Gy)照射後,給藥組的凋亡率均高于單照射組(F=16.97、76.65、92.86、209.66,P＜0.05),給藥組G2/M期細胞比例較單照射組增加(F=9.40、84.99、87.61、2025.85,P＜0.05).結論 TRAIL可以抑製Eca-109細胞的生長,誘導細胞凋亡和G2/M期阻滯,後者可能與TRAIL的放射增敏機製有關.
목적 연구조망소2배체(Apo-2 ligand,Apo2L),우칭종류배사인자상관조망유도배체(tumor necrosis factor-related apoptosis inducing ligand,TRAIL)대체외배양식관암세포주Eca-109방사민감성적영향.방법 MTT법검측TRAIL대Eca-109세포적독성；극륭형성실험검측TRAIL대Eca-109세포적방사증민작용；류식세포의(FCM)기술분석TRAIL대조망솔급세포주기적영향.결과 100～800 μg/ml TRAIL수농도승고대Eca-109세포적증식억제솔증대,약물농도여세포억제솔정상관(r=0.981,P＜0.01),50％억제농도(IC50)위637 μg/ml;200 μg/ml TRAIL능증가Eca-109세포적방사민감성,방사증민비위1.219；불동제량(0～8 Gy)조사후,급약조적조망솔균고우단조사조(F=16.97、76.65、92.86、209.66,P＜0.05),급약조G2/M기세포비례교단조사조증가(F=9.40、84.99、87.61、2025.85,P＜0.05).결론 TRAIL가이억제Eca-109세포적생장,유도세포조망화G2/M기조체,후자가능여TRAIL적방사증민궤제유관.
Objective To study the radiosensitive effect of Apo-2 ligand (Apo2L),known as tumor necrosis factor-related apoptosis inducing ligand (TRAIL),on esophageal cancer cell line-Ecal09 in vitro.Methods MTT assay and clonogenic assay were performed to evaluate the cytotoxicity and radiosensitization of TRAIL,respectively.Cell apoptosis and cell cycle distribution were measured by flow cytometry (FCM).Results TRAIL inhibited cell growth in a dose-dependent manner and its 50％ inhibition concentration(IC50) was 637 μg/ml.The concentration 200 μg/ml TRAIL could enhance cell radiosensitivity with a SER of 1.219.After 2,4,6,8 Gy X-ray irradiation,the incidence of apoptosis in TRAIL-treated cells was higher than that of irradiation alone groups(F =16.97,76.65,92.86,209.66,P ＜0.05).The percentage of cells in G2/M phase of TRAIL-treated groups was also higher than that of irradiation alone groups (F =9.40,84.99,87.61,2025.85,P ＜ 0.05).Conclusions TRAIL could inhibit cell growth,induce apoptosis and G2/M arrest,and had radiosensitization effect on Eca-109 cells.
