CAJ | 학술논문

目的观察电离辐射所致肠上皮细胞磷脂的变化,探讨放射性肠损伤的发生机制.方法将大鼠小肠上皮细胞(IEC-6)分为3组:正常对照组、8 GyX射线照射组和12 Gy X射线照射组.分别在照射后6和24 h,提取IEC-6细胞磷脂,利用液相色谱-质谱联用技术(HPLC-MS)测定辐射所致细胞磷脂的变化.结果辐射后6h,8 Gy照射组细胞磷脂未发生显著变化;12 Gy照射组细胞磷脂变化明显,其中磷脂酰甘油(PG)、磷脂酰肌醇(PI)和溶血磷脂酰胆碱(Lyso PC)均显著性上调(F=5.37、9.60、9.88,P＜0.05).而照射后24 h,两个辐射剂量组中多种磷脂酰乙醇胺(PE)和PG分子显著下调(F=5.15 ～99.77,P＜0.05),12 Gy照射组中多种神经鞘磷脂(SM)显著上调(F=4.35～7.92,P＜0.05).结论电离辐射可导致大鼠肠上皮细胞(IEC-6)磷脂代谢紊乱,其紊乱程度与辐射剂量相关.
목적 관찰전리복사소치장상피세포린지적변화,탐토방사성장손상적발생궤제.방법 장대서소장상피세포(IEC-6)분위3조:정상대조조、8 GyX사선조사조화12 Gy X사선조사조.분별재조사후6화24 h,제취IEC-6세포린지,이용액상색보-질보련용기술(HPLC-MS)측정복사소치세포린지적변화.결과 복사후6h,8 Gy조사조세포린지미발생현저변화;12 Gy조사조세포린지변화명현,기중린지선감유(PG)、린지선기순(PI)화용혈린지선담감(Lyso PC)균현저성상조(F=5.37、9.60、9.88,P＜0.05).이조사후24 h,량개복사제량조중다충린지선을순알(PE)화PG분자현저하조(F=5.15 ～99.77,P＜0.05),12 Gy조사조중다충신경초린지(SM)현저상조(F=4.35～7.92,P＜0.05).결론 전리복사가도치대서장상피세포(IEC-6)린지대사문란,기문란정도여복사제량상관.
Objective To investigate radiation-induced alternations of phospholipids in epithelial cells,and to provide experimental evidence for understanding the mechanism of radiation-induced intestinal injury.Methods The intestinal epithelial cells(IEC-6)in rats were divided into three groups:normal control group,8 Gy X-ray irradiation group and 12 Gy X-ray irradiation group.Phospholipids were extracted at 6 h or 24 h after radiation and then measured by high-performance liquid chromatography and mass spectrometry(HPLC-MS).Results At 6 h after radiation,the phospholipids in 8 Gy irradiation group didn't vary significantly,while those in 12 Gy irradiation group changed.The PG,PI and Lyso PC were significantly up-regulated(F=5.37,9.60,9.88,P＜0.05).However,at 24 h after radiation,many PE and PG species in both irradiation groups declined(F=5.15-99.77,P＜0.05)and SM species increased in 12 Gy irradiation group(F=4.35-7.92,P＜0.05).Conclusions The ionizing radiation could disorder phospholipid metabolism in IEC-6 cells with a dose-dependent manner.