中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2013年
5期
468-471
,共4页
周慧%杨冰%唐泉%孙元明%韩英%樊飞跃%贾立立
週慧%楊冰%唐泉%孫元明%韓英%樊飛躍%賈立立
주혜%양빙%당천%손원명%한영%번비약%가립립
电离辐射%成骨细胞%破骨细胞%核因子κB受体活化因子配体%骨保护素
電離輻射%成骨細胞%破骨細胞%覈因子κB受體活化因子配體%骨保護素
전리복사%성골세포%파골세포%핵인자κB수체활화인자배체%골보호소
Ionizing radiation%Osteoblasts%Osteoclasts%Receptor activator for NF-κB ligand%Osteoprotegerin
目的 研究电离辐射对成骨细胞RANKL和OPG表达的影响,探讨辐射导致骨损伤的分子机制.方法 采用MC3T3-E1细胞诱导分化为成骨细胞,经O、2和4 Gy137 Cs γ射线照射后,采用实时定量PCR及Western blot方法检测电离辐射对于成骨细胞RANKL和OPG mRNA及其蛋白水平表达的影响.结果 4 Gy照射可以导致成骨细胞RANKL mRNA(t=5.41,P＜0.05)及其蛋白(t=68.37,P＜0.01)表达水平上调;2和4 Gy照射可以导致成骨细胞OPG mRNA(t=5.20、7.02,P＜0.05)及其蛋白(=7.78、9.45,P＜0.05)表达水平下调.结论 2和4 Gy电离辐射可以导致成骨细胞中RANKL/RANK/OPG通路发生改变,促进破骨细胞的分化和成熟,进而促进破骨细胞的骨吸收作用.
目的 研究電離輻射對成骨細胞RANKL和OPG錶達的影響,探討輻射導緻骨損傷的分子機製.方法 採用MC3T3-E1細胞誘導分化為成骨細胞,經O、2和4 Gy137 Cs γ射線照射後,採用實時定量PCR及Western blot方法檢測電離輻射對于成骨細胞RANKL和OPG mRNA及其蛋白水平錶達的影響.結果 4 Gy照射可以導緻成骨細胞RANKL mRNA(t=5.41,P＜0.05)及其蛋白(t=68.37,P＜0.01)錶達水平上調;2和4 Gy照射可以導緻成骨細胞OPG mRNA(t=5.20、7.02,P＜0.05)及其蛋白(=7.78、9.45,P＜0.05)錶達水平下調.結論 2和4 Gy電離輻射可以導緻成骨細胞中RANKL/RANK/OPG通路髮生改變,促進破骨細胞的分化和成熟,進而促進破骨細胞的骨吸收作用.
목적 연구전리복사대성골세포RANKL화OPG표체적영향,탐토복사도치골손상적분자궤제.방법 채용MC3T3-E1세포유도분화위성골세포,경O、2화4 Gy137 Cs γ사선조사후,채용실시정량PCR급Western blot방법검측전리복사대우성골세포RANKL화OPG mRNA급기단백수평표체적영향.결과 4 Gy조사가이도치성골세포RANKL mRNA(t=5.41,P＜0.05)급기단백(t=68.37,P＜0.01)표체수평상조;2화4 Gy조사가이도치성골세포OPG mRNA(t=5.20、7.02,P＜0.05)급기단백(=7.78、9.45,P＜0.05)표체수평하조.결론 2화4 Gy전리복사가이도치성골세포중RANKL/RANK/OPG통로발생개변,촉진파골세포적분화화성숙,진이촉진파골세포적골흡수작용.
Objective To investigate the effect of radiation on the expressions of RANKL and OPG in osteoblasts in order to disclose the molecular mechanism of bone injury induced by ionizing radiation.Methods The osteoblasts were differentiated from MC3T3-E1 cells.After 2 or 4 Gy137 Cs γ-irradiation,the mRNA and protein expression levels of RANKL and OPG of osteoblast precursor and osteoblast were detected by real-time PCR and Western blot.Results The expressions of RANKL mRNA (t=5.41,P＜0.05)and protein(t=68.37,P＜0.01)were up-regulated after 4 Gy irradiation,while the expressions of OPG mRNA(t=5.20,7.02,P＜0.05)and protein(t=7.78,9.45,P＜0.05)were down-regulated after 2 and 4 Gy irradiation.Conclusions 2 and 4 Gy ionizing radiation alters RANKL/RANK/OPG pathway in osteoblasts,which may promote the osteoclast differentiation and maturation and hence promote bone resorption of osteoclasts.
