中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2013年
5期
484-488
,共5页
杨百霞%杨曦%朱琪伟%吴志军%翟小刚%夏小春%蔡晶
楊百霞%楊晞%硃琪偉%吳誌軍%翟小剛%夏小春%蔡晶
양백하%양희%주기위%오지군%적소강%하소춘%채정
盐酸小檗碱%食管癌%乏氧%HIF-1%放射敏感性
鹽痠小檗堿%食管癌%乏氧%HIF-1%放射敏感性
염산소벽감%식관암%핍양%HIF-1%방사민감성
Berberine%Esophageal cancer%Hypoxia%HIF-1%Radiosensitivity
目的 研究盐酸小檗碱对乏氧食管癌细胞的放射增敏作用.方法 通过MTT法检测盐酸小檗碱对食管癌ECA-109细胞生长的抑制;克隆集落形成实验观察盐酸小檗碱的放射增敏作用;通过免疫荧光实验观察HIF-1的表达情况;流式细胞仪检测细胞的凋亡情况;Western blot检测细胞内HIF-1表达;γ-H2AX焦点形成检测DNA分子损伤情况.结果 盐酸小檗碱抑制食管癌ECA-109细胞的生长,并且有明显的时间和剂量依赖性;通过单击多靶模型拟合曲线,可见低浓度盐酸小檗碱预处理24 h,相比于照射组,可以增加乏氧ECA-109细胞的辐射敏感性(t=3.69,P＜0.05),辐射增敏指数为1.42;与照射组相比,盐酸小檗碱+照射组中的细胞凋亡率明显增加(t=4.74,P＜0.05);盐酸小檗碱作用于乏氧食管癌细胞,可以使乏氧相关蛋白HIF-1的表达降低,并且呈现明显的量效关系;相对于照射组,盐酸小檗碱+照射组能够增加DNA双链断裂数目(DSB).结论 盐酸小檗碱能够提高食管癌细胞的放射敏感性,与其增加食管癌细胞的凋亡率和降低乏氧食管癌ECA-109细胞内HIF-1的表达有关.
目的 研究鹽痠小檗堿對乏氧食管癌細胞的放射增敏作用.方法 通過MTT法檢測鹽痠小檗堿對食管癌ECA-109細胞生長的抑製;剋隆集落形成實驗觀察鹽痠小檗堿的放射增敏作用;通過免疫熒光實驗觀察HIF-1的錶達情況;流式細胞儀檢測細胞的凋亡情況;Western blot檢測細胞內HIF-1錶達;γ-H2AX焦點形成檢測DNA分子損傷情況.結果 鹽痠小檗堿抑製食管癌ECA-109細胞的生長,併且有明顯的時間和劑量依賴性;通過單擊多靶模型擬閤麯線,可見低濃度鹽痠小檗堿預處理24 h,相比于照射組,可以增加乏氧ECA-109細胞的輻射敏感性(t=3.69,P＜0.05),輻射增敏指數為1.42;與照射組相比,鹽痠小檗堿+照射組中的細胞凋亡率明顯增加(t=4.74,P＜0.05);鹽痠小檗堿作用于乏氧食管癌細胞,可以使乏氧相關蛋白HIF-1的錶達降低,併且呈現明顯的量效關繫;相對于照射組,鹽痠小檗堿+照射組能夠增加DNA雙鏈斷裂數目(DSB).結論 鹽痠小檗堿能夠提高食管癌細胞的放射敏感性,與其增加食管癌細胞的凋亡率和降低乏氧食管癌ECA-109細胞內HIF-1的錶達有關.
목적 연구염산소벽감대핍양식관암세포적방사증민작용.방법 통과MTT법검측염산소벽감대식관암ECA-109세포생장적억제;극륭집락형성실험관찰염산소벽감적방사증민작용;통과면역형광실험관찰HIF-1적표체정황;류식세포의검측세포적조망정황;Western blot검측세포내HIF-1표체;γ-H2AX초점형성검측DNA분자손상정황.결과 염산소벽감억제식관암ECA-109세포적생장,병차유명현적시간화제량의뢰성;통과단격다파모형의합곡선,가견저농도염산소벽감예처리24 h,상비우조사조,가이증가핍양ECA-109세포적복사민감성(t=3.69,P＜0.05),복사증민지수위1.42;여조사조상비,염산소벽감+조사조중적세포조망솔명현증가(t=4.74,P＜0.05);염산소벽감작용우핍양식관암세포,가이사핍양상관단백HIF-1적표체강저,병차정현명현적량효관계;상대우조사조,염산소벽감+조사조능구증가DNA쌍련단렬수목(DSB).결론 염산소벽감능구제고식관암세포적방사민감성,여기증가식관암세포적조망솔화강저핍양식관암ECA-109세포내HIF-1적표체유관.
Objective To explore the radiosensitivity of berberine on esophageal cancer cells under hypoxia condition.Methods MTT assay and clonogenic survival assay were used to evaluate the effect of berberine on proliferation inhibition and radiosensitivity of esophageal cancer cells,respectively.Immunofluorescence was employed to examine the expression of HIF-1.The change of cell cycle distribution and cell apoptosis was assayed by flow cytometry.The expression of HIF-1 was measured by Western blot.DNA damage was detected by γ-H2AX Foci counting.Results With a clear dose and time effect,berberine inhibited cell proliferation and enhanced cell radiosensitivity(t =3.69,P＜0.05)with a sensitizing enhancement ratio(SER)of 1.42.Berberine caused a dose-dependent decrease in HIF-1 protein expression and also significantly increased the cell apoptosis in ECA-109 population(t=4.74,P＜0.05).Compared with the radiation alone group,berberine enhanced X-ray induced DNA double chain breaks(DSB).Conclusions Berberine can increase the radiosensitivity of esophageal cell line ECA-109,which may be associated with decrease of HIF-1 expression and induction of apoptosis in ECA-109 cells.
