CAJ | 학술논문

目的探讨顺铂联合X射线照射对鼻咽高分化鳞癌(CNE-1)裸鼠移植瘤抑瘤率和CyclinB1、CyclinD1转录水平的影响.方法建立CNE-1裸鼠移植肿瘤模型后随机分成对照、顺铂、急速照射、模拟调强放疗(IMRT)和模拟IMRT加顺铂组,照射剂量为单次20 Gy,顺铂剂量为15μg/g体重.绘制肿瘤生长曲线,计算肿瘤抑制率,并用RT-PCR方法测定各组肿瘤组织中CyclinB1、CyclinD1的转录水平.组间比较行单因素方差分析.结果对照、顺铂、急速照射、模拟IMRT和模拟IMRT加顺铂组肿瘤抑制率分别为-129.1％、-71.2％、42.5％、35.3％和47.1％[急速照射与模拟IMRT组不同(P =0.034),但急速照射与模拟IMRT加顺铂组相似(P=0.222)]；CyclinB1转录水平分别为0.429、0.386、0.322、0.354和0.268[急速照射与模拟IMRT组、模拟IMRT加顺铂组均不同(P=0.007、0.000)]；CyclinD1转录水平分别为0.716、0.583、0.348、0.495和0.296[急速照射与模拟IMRT组不同(P =0.000),但急速照射与模拟IMRT加顺铂组相似(P=0.072)].结论 20 Gy单次X射线照射和加顺铂均能缩小肿瘤体积,但单用顺铂仅能延缓生长；20 Gy单次X射线照射和加顺铂以及单纯顺铂均可致CyclinBl、CyclinD1转录水平下调；而模拟IMRT因时间延长可使肿瘤抑制率下降和CyclinB1、CyclinD1下调减弱,但联合顺铂后或许能补偿这种降低效应.
목적 탐토순박연합X사선조사대비인고분화린암(CNE-1)라서이식류억류솔화CyclinB1、CyclinD1전록수평적영향.방법 건립CNE-1라서이식종류모형후수궤분성대조、순박、급속조사、모의조강방료(IMRT)화모의IMRT가순박조,조사제량위단차20 Gy,순박제량위15μg/g체중.회제종류생장곡선,계산종류억제솔,병용RT-PCR방법측정각조종류조직중CyclinB1、CyclinD1적전록수평.조간비교행단인소방차분석.결과 대조、순박、급속조사、모의IMRT화모의IMRT가순박조종류억제솔분별위-129.1％、-71.2％、42.5％、35.3％화47.1％[급속조사여모의IMRT조불동(P =0.034),단급속조사여모의IMRT가순박조상사(P=0.222)]；CyclinB1전록수평분별위0.429、0.386、0.322、0.354화0.268[급속조사여모의IMRT조、모의IMRT가순박조균불동(P=0.007、0.000)]；CyclinD1전록수평분별위0.716、0.583、0.348、0.495화0.296[급속조사여모의IMRT조불동(P =0.000),단급속조사여모의IMRT가순박조상사(P=0.072)].결론 20 Gy단차X사선조사화가순박균능축소종류체적,단단용순박부능연완생장；20 Gy단차X사선조사화가순박이급단순순박균가치CyclinBl、CyclinD1전록수평하조；이모의IMRT인시간연장가사종류억제솔하강화CyclinB1、CyclinD1하조감약,단연합순박후혹허능보상저충강저효응.
Objective To investigate influence of cisplatin (DDP) on the tumor inhibition rate,transcriptional levels of CyclinB1 and CyclinD1 of CNE-1 xenograft in nude mice.Methods Tumor mode of nude mice CNE-1 xenograft was established.Then mice were divided into control arm,DDP arm,high speed irradiation arm,simulated intensity modulated radiation therapy (IMRT) arm and simulated IMRT + DDP arm,with 12 mice in each arm.Irradiation dose was 20 Gy with a single fraction.DDP was 15 μg/g weight.The maximum diameter of tumor base was measured every other day.The growth curve was drawn and tumor inhibition rate werevcalculated after 40 days.The transcriptional level of CyclinB1 and CyclinD1 of xenograft was measured by RT-PCR.The results of different groups were compared with one-factor analysis of variance.Results Tumor inhibition rates of the control arm,DDP arm,high speed irradiation arm,simulated IMRT arm and simulated IMRT + DDP arm were-129.1％,-71.2％,42.5％,35.3％ and 47.1％,respectively.There was significant difference between the high speed irradiation arm and simulated IMRT arm (P =0.034),but not between the high speed irradiation arm and simulated IMRT + DDP arm (P =0.222).The transcriptional levels of CyclinB1 in the arms were 0.429,0.386,0.322,0.354 and 0.268.There were significant differences between the high speed irradiation arm and the simulated IMRT arm or the simulated IMRT + DDP arm (P =0.007 and 0.000).The transcriptional levels of CyclinD1 in the arms were 0.716,0.583,0.348,0.495 and 0.296,respectively.There was significant difference between the acute irradiation arm and the simulated IMRT arm (P =0.000),but there was no significant difference between the high speed irradiation arm and the simulated IMRT + DDP arm (P =0.072).Conclusions Irradiation of 20 Gy single fraction,or combined with DDP are effective on the CNE-1 xenograft in nude mice,but DDP alone can only lower the tumor growth speed.Irradiation of 20 Gy single fraction,or combined with DDP,or DDP alone can reduce the transcriptional levels of CyclinB1 and CyclinD1.As the single therapeutic time is prolonged in IMRT mode,the tumor inhibition rate is reduce,and the reduce of the transcriptional levels of CyclinB1 and CyclinD1 is depressed,while combined DDP can compensate the decline of the biological effect.