CAJ | 학술논문

目的筛选乳腺癌亚临床肿瘤相对优化剂量分割方案.方法采用人乳腺癌裸小鼠移植瘤亚临床肿瘤为实验模型,细胞接种后72 h进行常规分割(200 cGy/次、1次/d、5次/周),超分割(160 cGy/次、2次/d、间隔6h、10次/周),大分割(300 cGy/次、1次/d、5次/周,400 cGy/次、隔天1次、3次/周)照射,总剂量为40、60 Gy.观察指标成瘤率、近期肿瘤控制率、远期肿瘤控制率等.对各实验组测量数据差异行x2检验.结果总剂量40 Gy时(接种细胞数1.5 ×105,空白对照组成瘤率为2/8),超分割方案是相对优化方案.总剂量60 Gy时(接种细胞数3.1 ×105,空白对照组成瘤率为11/11),大分割300 cGy 5次/周最优(P =0.001),其中常规分割组近、远期肿瘤控制率均为0(成瘤率均为8/8),超分割组近、远期肿瘤控制率分别为50％、25％(成瘤比率分别为4/8、6/8),大分割400cGy 3次/周组近、远期肿瘤控制率均为25％(成瘤率均为6/8),大分割300 cGy 5次/周组近、远期肿瘤控制率均为67％(成瘤率均为4/12).结论接种细胞数不同,控制亚临床肿瘤所需分割方案及总剂量不同.当成瘤率为100％时,就远期控制而言,300 cGy 5次/周是相对优化方案.
목적 사선유선암아림상종류상대우화제량분할방안.방법 채용인유선암라소서이식류아림상종류위실험모형,세포접충후72 h진행상규분할(200 cGy/차、1차/d、5차/주),초분할(160 cGy/차、2차/d、간격6h、10차/주),대분할(300 cGy/차、1차/d、5차/주,400 cGy/차、격천1차、3차/주)조사,총제량위40、60 Gy.관찰지표성류솔、근기종류공제솔、원기종류공제솔등.대각실험조측량수거차이행x2검험.결과 총제량40 Gy시(접충세포수1.5 ×105,공백대조조성류솔위2/8),초분할방안시상대우화방안.총제량60 Gy시(접충세포수3.1 ×105,공백대조조성류솔위11/11),대분할300 cGy 5차/주최우(P =0.001),기중상규분할조근、원기종류공제솔균위0(성류솔균위8/8),초분할조근、원기종류공제솔분별위50％、25％(성류비솔분별위4/8、6/8),대분할400cGy 3차/주조근、원기종류공제솔균위25％(성류솔균위6/8),대분할300 cGy 5차/주조근、원기종류공제솔균위67％(성류솔균위4/12).결론 접충세포수불동,공제아림상종류소수분할방안급총제량불동.당성류솔위100％시,취원기공제이언,300 cGy 5차/주시상대우화방안.
Objective To determine the optimized fractionated radiation schedule by comparing the dose-response relationship between different fractionated radiation schedules with a total dose of 40 Gy or 60 Gy in subclinical breast tumor.Methods Balb/c nude mice bearing subclinical human breast cancer (injected subcutaneously into the hind legs with 1.5 × 105 or 3.1 × 105 exponentially growing MCF-7 cells) were assigned randomly to blank control group (without radiation),conventionally fractionated radiation group (200 cGy,once daily,10 times/week),hyperfractionated radiation group (160 cGy,twice daily with an interval of 6 h,5 times/week),first hypofractionated radiation group (300 cGy,once daily,5 times/ week),and second hypofractionated radiation group (400 cGy,once every other day,3 times/week) ;the total dose was 40 Gy or 60 Gy.The measurement indices were tumor formation rate,short-term tumor control rate,long-term tumor control rate,the time of tumor recurrence,and the maximum diameter of the bottom of tumor.The observation lasted 24 weeks.Data were compared between these groups by chi-square test.Results With a total dose of 40 Gy (the number of injected cells was 1.5 × 105,the tumor formation rate of the blank control group was 2/8),hyperfractionated radiation was the optimized schedule.With a total dose of 60 Gy (the number of injected cells was 3.1 × 105,the tumor formation rate of the blank control group was 11/11),the first hypofractionated radiation (300 cGy,once daily,5 times/week) was the optimized schedule (P =0.001);the short-term and long-term tumor control rates of the conventionally fractionated radiation group,hyperfractionated radiation group,second hypofractionated radiation group,and first hypofractionated radiation group were 0/0 (tumor formation rates:8/8 and 8/8),50％/25％ (tumor formation rates:4/8 and 6/8),25 ％/25 ％ (tumor formation rates:6/8 and 6/8)),and 67 ％/67 ％ (tumor formation rates:4/12 and 4/12),respectively.Conclusions The optimized fractionated radiation schedule for subclinical breast cancer and its total dose vary with the number of injected tumor cells.When the tumor formation rate is 100％,hypofractionated radiation (300 cGy,once daily,5 times/week) is the optimized schedule in terms of long-term tumor control.