中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2012年
11期
859-862
,共4页
徐怀勇%巩本刚%高崇崇%李梦宇%吴俊本%相亭海%成丕光
徐懷勇%鞏本剛%高崇崇%李夢宇%吳俊本%相亭海%成丕光
서부용%공본강%고숭숭%리몽우%오준본%상정해%성비광
胰腺肿瘤%蛋白酶抑制药%基质金属蛋白酶%细胞凋亡
胰腺腫瘤%蛋白酶抑製藥%基質金屬蛋白酶%細胞凋亡
이선종류%단백매억제약%기질금속단백매%세포조망
Pancreatic neoplasms%Protease inhibitors%Matrix metalloproteinases%Apoptosis
目的 观察新型选择性基质金属蛋白酶(MMP)抑制剂MMI-166对裸鼠人胰腺癌细胞SW1990移植瘤生长及瘤内MMP-2、MMP-9蛋白表达和细胞凋亡的影响.方法 应用人胰腺癌细胞株SW1990建立裸鼠胰腺癌皮下移植瘤模型.裸鼠随机分为对照组和实验组.对照组口服生理盐水,实验组口服MMI-166(200 mg·kg-1·d-1).游标卡尺测量肿瘤的长、短径,比较两组间肿瘤体积、抑瘤率.采用免疫组织化学测定肿瘤组织内MMP-2、MMP-9蛋白的表达.利用脱氧核苷酸末端转移酶介导缺口末端标记法(TUNEL法)检测细胞凋亡指数(AI).结果 研究结束后,实验组移植瘤肿瘤体积为(1252.30±464.84)mm3,明显小于对照组移植瘤肿瘤的(2241.82±208.06)mm3;实验组瘤体重(1.42±0.15)g,低于对照组的瘤体重[(2.17±0.20)g]；实验组抑瘤率为34.47％.实验组内蛋白MMP-2和MMP-9的表达分别为(2.80±1.10)、(2.60±1.52),与对照组相比显著降低；细胞凋亡指数在实验组为(75.60±9.71)％,显著高于对照组的(17.40±10.14)％.结论 MMI-166可抑制胰腺癌肿瘤的生长并诱导细胞凋亡,其机制可能与抑制MMP-2、MMP-9蛋白表达有关.
目的 觀察新型選擇性基質金屬蛋白酶(MMP)抑製劑MMI-166對裸鼠人胰腺癌細胞SW1990移植瘤生長及瘤內MMP-2、MMP-9蛋白錶達和細胞凋亡的影響.方法 應用人胰腺癌細胞株SW1990建立裸鼠胰腺癌皮下移植瘤模型.裸鼠隨機分為對照組和實驗組.對照組口服生理鹽水,實驗組口服MMI-166(200 mg·kg-1·d-1).遊標卡呎測量腫瘤的長、短徑,比較兩組間腫瘤體積、抑瘤率.採用免疫組織化學測定腫瘤組織內MMP-2、MMP-9蛋白的錶達.利用脫氧覈苷痠末耑轉移酶介導缺口末耑標記法(TUNEL法)檢測細胞凋亡指數(AI).結果 研究結束後,實驗組移植瘤腫瘤體積為(1252.30±464.84)mm3,明顯小于對照組移植瘤腫瘤的(2241.82±208.06)mm3;實驗組瘤體重(1.42±0.15)g,低于對照組的瘤體重[(2.17±0.20)g]；實驗組抑瘤率為34.47％.實驗組內蛋白MMP-2和MMP-9的錶達分彆為(2.80±1.10)、(2.60±1.52),與對照組相比顯著降低；細胞凋亡指數在實驗組為(75.60±9.71)％,顯著高于對照組的(17.40±10.14)％.結論 MMI-166可抑製胰腺癌腫瘤的生長併誘導細胞凋亡,其機製可能與抑製MMP-2、MMP-9蛋白錶達有關.
목적 관찰신형선택성기질금속단백매(MMP)억제제MMI-166대라서인이선암세포SW1990이식류생장급류내MMP-2、MMP-9단백표체화세포조망적영향.방법 응용인이선암세포주SW1990건립라서이선암피하이식류모형.라서수궤분위대조조화실험조.대조조구복생리염수,실험조구복MMI-166(200 mg·kg-1·d-1).유표잡척측량종류적장、단경,비교량조간종류체적、억류솔.채용면역조직화학측정종류조직내MMP-2、MMP-9단백적표체.이용탈양핵감산말단전이매개도결구말단표기법(TUNEL법)검측세포조망지수(AI).결과 연구결속후,실험조이식류종류체적위(1252.30±464.84)mm3,명현소우대조조이식류종류적(2241.82±208.06)mm3;실험조류체중(1.42±0.15)g,저우대조조적류체중[(2.17±0.20)g]；실험조억류솔위34.47％.실험조내단백MMP-2화MMP-9적표체분별위(2.80±1.10)、(2.60±1.52),여대조조상비현저강저；세포조망지수재실험조위(75.60±9.71)％,현저고우대조조적(17.40±10.14)％.결론 MMI-166가억제이선암종류적생장병유도세포조망,기궤제가능여억제MMP-2、MMP-9단백표체유관.
Objective To investigate of the MMI-166 on the expression of MMP-2,MMP-9 and the cell apoptosis of nude mouse xenografts of SW1990 human pancreatic cancer cells.Methods Establishment of control and experimental groups,randomly,the human pancreatic cancer xenograft model of SW1990 was constructed.The control group was treated with normal saline,and experimental group was treated with MML-166 (200 mg · kg-1 · d-1).The tumor volume and tumor inhibition rate was measured by vernier caliper through length and short diameter.The expression of MMP-2 and MMP-9 protein was observed using immunohistochemistry in the tumor tissues.Apoptosis index was detected by deoxynucleotidyl transferase-mediated nick end labeling (TUNEL method).Results The tumor volume of MMI-166 group (1252.30± 464.84) mm3 was less than the control group (2241.82±208.06) mm3,significantly.The inhibition rate was 34.47％ between the experimental groups (treat with MMI-166) (1.42±0.15) g and control group (2.17±0.20) g.The expression of MMP-2 (2.80 ± 1.10) ％ and MMP-9 (2.60 ± 1.52) ％ protein was significantly downregulated in MMI-166 group,compared with the control group.Apoptotic index in the experimental group (75.60±9.71) ％ was higher than the control group (17.40 ± 10.14) ％,significantly.Conclusion The mechanism of MMI-166 inhibiting pancreatic tumor growth and inducing apoptosis may be related to the suppression of MMP-2 and MMP-9 protein expression.
