CAJ | 학술논문

目的观察动脉持续灌注化疗状态下,异甘草酸镁与不同化疗药按不同次序给药对肝癌细胞系HepG-2和肝细胞系7702的影响.方法采用MTT法,在体外培养的HepG-2和7702细胞培养液中,分别检测10种化疗药物与异甘草酸镁联用/序贯使用时,对不同细胞系的抑制率,同时观察Mg2+与化疗药共同孵育对HepG-2细胞系的作用.结果异甘草酸镁对泰索帝、健择、培美曲塞二钠、表柔比星、5-Fu等有协同作用.这种协同作用在异甘草酸镁提前给药或者与化疗药共同孵育时更为明显.4种化疗药与硫酸镁联合有轻度的协同作用.结论异甘草酸镁与部分化疗药联用表现出较明显的协同作用,且不同的给药顺序对药物间相互作用有很大影响.其机理需进一步明确.
목적 관찰동맥지속관주화료상태하,이감초산미여불동화료약안불동차서급약대간암세포계HepG-2화간세포계7702적영향.방법 채용MTT법,재체외배양적HepG-2화7702세포배양액중,분별검측10충화료약물여이감초산미련용/서관사용시,대불동세포계적억제솔,동시관찰Mg2+여화료약공동부육대HepG-2세포계적작용.결과 이감초산미대태색제、건택、배미곡새이납、표유비성、5-Fu등유협동작용.저충협동작용재이감초산미제전급약혹자여화료약공동부육시경위명현.4충화료약여류산미연합유경도적협동작용.결론 이감초산미여부분화료약련용표현출교명현적협동작용,차불동적급약순서대약물간상호작용유흔대영향.기궤리수진일보명학.
Objective Under the circumstance of continuous arterial infusion,role of magnesium isoglycyrrhizinate combined with different kinds of chemotherapy agents respectively were detected on the HepG-2 and 7702cell lines.Method MTT assay in cultured HepG-2 and 7702 in vitro were used to detect different chemotherapy agents combined with transemetil with different sequence; interaction of MgSO4 with chemotherapy agents were observed in HepG-2 cell lines.Results When combined with docetaxel,gemcitabine,epirubicin,5 fluorouracil,pemetrexed disodium,magnesium isoglycyrrhizinate had synergistic effect; when combined with other agents,there were no antagonism effect; 4 kinds of chemotherapy agents had slight synergistic effect with magnesium sulfate.Conclusion Magnesium isoglycyrrhizinate showed obvious synergistic effect when combined with chemotherapy agents; different sequence between magnesium isoglycyrrhizinate and chemotherapy agents showed different effect; the mechanism need more study.