CAJ | 학술논문

目的探讨核仁纺锤体相关蛋白1(NuSAP1)在肝细胞癌组织及非癌组织中的表达及其与早期复发和预后的关系.方法应用RT-PCR、荧光定量PCR和免疫组织化学方法检测61例肝细胞癌组织及相应非癌组织中NuSAP1的表达,分析NuSAP1在肝细胞癌组织中的表达及其与相关临床病理参数的关系.结果无论是核酸水平还是蛋白水平,NuSAP1在肝细胞癌组织中的表达均明显高于非癌组织(P＜0.05).单因素分析表明:肝细胞癌组织中NuSAP1 mRNA与蛋白的表达均与TNM分期、淋巴结转移、早期复发、有无癌栓和病理分化相关(P＜0.05).多因素逐步Logistic 回归分析表明:NuSAP1蛋白的表达是早期复发的独立影响因素(P＜0.05).生存分析表明NuSAP1在肝细胞癌组织中的高表达与肝细胞癌患者的预后差相关(x2=15.846,P=0.000).结论 Nu-SAP1在肝细胞癌组织中存在过表达,NuSAP1在肝细胞癌中的过表达与肝细胞癌患者的早期复发有关,NuSAP1高表达者预后较差.
목적 탐토핵인방추체상관단백1(NuSAP1)재간세포암조직급비암조직중적표체급기여조기복발화예후적관계.방법 응용RT-PCR、형광정량PCR화면역조직화학방법검측61례간세포암조직급상응비암조직중NuSAP1적표체,분석NuSAP1재간세포암조직중적표체급기여상관림상병리삼수적관계.결과 무론시핵산수평환시단백수평,NuSAP1재간세포암조직중적표체균명현고우비암조직(P＜0.05).단인소분석표명:간세포암조직중NuSAP1 mRNA여단백적표체균여TNM분기、림파결전이、조기복발、유무암전화병리분화상관(P＜0.05).다인소축보Logistic 회귀분석표명:NuSAP1단백적표체시조기복발적독립영향인소(P＜0.05).생존분석표명NuSAP1재간세포암조직중적고표체여간세포암환자적예후차상관(x2=15.846,P=0.000).결론 Nu-SAP1재간세포암조직중존재과표체,NuSAP1재간세포암중적과표체여간세포암환자적조기복발유관,NuSAP1고표체자예후교차.
Objective To investigate the expression of nucleolar spindle-associated protein 1(NuSAP1) in hepatocellular carcinoma (HCC) and in non-cancerous tissues,and to study the correlation between NuSAP1 and early recurrence and prognosis of HCC.Methods The expression of NuSAP1 in 61 cases of HCC and non-cancerous tissues were assessed by RT-PCR,quantitative PCR and immunohistochemistry.The relationship between the expression and the clinicopathological features was studied.Results The levels of mRNA and protein in HCC were higher than the non-cancerous tissues (P＜0.05).On univariate analysis,the expression levels of NuSAP1,mRNA and protein in HCC were significantly associated with TNM classification,lymphatic metastasis,early recurrence,tumor thrombosis and histological differentiation (P＜0.05).Multivariate analysis showed early recurrence was associated with the expression of NuSAP1 protein in HCC (P＜0.05).Overexpression of NuSAP1 protein was correlated with poor outcome of the patients with HCC (x2=15.846,P＜0.001).Conclusions NuSAP1 was overexpressed in hepatocellular carcinoma.Overexpression of NuSAP1 was associated with early postoperative HCC recurrence and bad prognosis.